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661.
有2种不同方法制备的H2S标准溶液进行测定,结果表明,所得标准曲线斜率一致,因此,在实际工作中可对其灵活运用。 相似文献
662.
Majagi SH Vijaykumar K Vasanthkaumar B 《Environmental monitoring and assessment》2008,138(1-3):273-279
In this study, some heavy metals concentrations (Zn, Pb, Fe, Mn, Cu, Ni) and other physico-chemical parameters were studied
during October 2001 to September 2003 in Karanja reservoir, Bidar district. Water quality parameters were collected monthly
basis whereas heavy metals were analyzed by Atomic Adsorption Spectrometer (AAS). Heavy metals have shown within the permissible
limits, except Fe and Ni were recorded higher values in southwest monsoon, where as Mn has showed higher concentration in
northeast monsoon in and summer. All other physico-chemical parameters are with in the permissible limit. Water is moderately
hard and reservoir is productive. 相似文献
663.
Fecal source tracking by antibiotic resistance analysis on a watershed exhibiting low resistance 总被引:2,自引:0,他引:2
The ongoing development of microbial source tracking has made it possible to identify contamination sources with varying accuracy,
depending on the method used. The purpose of this study was to test the efficiency of the antibiotic resistance analysis (ARA)
method under low resistance by tracking the fecal sources at Turkey Creek, Oklahoma exhibiting this condition. The resistance
patterns of 772 water-isolates, tested with nine antibiotics, were analyzed by discriminant analysis (DA) utilizing a five-source
library containing 2250 isolates. The library passed various representativeness tests; however, two of the pulled-sample tests
suggested insufficient sampling. The resubstitution test of the library individual sources showed significant isolate misclassification
with an average rate of correct classification (ARCC) of 58%. These misclassifications were explained by low antibiotic resistance
(Wilcoxon test P < 0.0001). Seasonal DA of stream E. coli isolates for the pooled sources human/livestock/deer indicated that in fall, the human source dominated (P < 0.0001) at a rate of 56%, and that human and livestock respective contributions in winter (35 and 39%), spring (43 and
40%), and summer (37 and 35%) were similar. Deer scored lower (17–28%) than human and livestock at every season. The DA was
revised using results from a misclassification analysis to provide a perspective of the effect caused by low antibiotic resistance
and a more realistic determination of the fecal source rates at Turkey Creek. The revision increased livestock rates by 13–14%
(0.04 ≤ P ≤ 0.06), and decreased human and deer by 6–7%. Negative misclassification into livestock was significant (0.04 ≤ P ≤ 0.06). Low antibiotic resistance showed the greatest effect in this category. 相似文献
664.
Two strains of Pseudomonas species (B2 and D5)selected from an array of lead solubilizing and accumulatingbacteria obtained from the effluent contaminated soil samples of abattery manufacturing factory were studied. Increase in pH between 4.0 and 6.0 favoured the growth of isolates: Peaklog10 cfu mL–1 values of 7.1, 7.5 and 8.5 wereobtained at pH 4, 5 and 6, respectively.Cell bound lead concentrations for B2 (0.34 mg mL–1)and D5 (0.30 mg mL–1) obtained by direct contact withPbs were greater than lead concentrations of 0.89 and 0.25 mg mL–1 for B2 and D5, respectively,obtained in dialyzed cultures. These cell bound lead concentration in undialyzed cultures were alsogreater than lead concentrations of 0.03 and 0.07 mg mL–1 for B2 and D5 in culture supernatants. Glucose addition did nor improve lead accumulation in the isolates.Exploitation of such isolates for the biotreatment of lead ladeneffluent was conducted. 相似文献
665.
666.
以铜绿微囊藻(Microcystis aeruginosa)16S rRNA基因片段为靶序列设计一对特异性引物,采用Real-time PCR法,对铜绿微囊藻进行定性、定量检测。试验表明,仅含铜绿微囊藻DNA模板的样品有特异性扩增,扩增产物熔解曲线平稳,峰尖且窄,熔解温度为(87±1)℃。以重组质粒pMD-18T-16S为标准品,检测区间为1.1×102 copies/mL~1.1×108 copies/mL,所得标准曲线符合制备实时定量PCR标准曲线的要求,对标准品进行测定,方法检出限为11 copies/mL。用该标准曲线对实验室培养获得的铜绿微囊藻DNA样品进行定量检测,与显微镜计数结果基本一致。 相似文献
667.
Dickella Gamaralalage Jagath Premakumara Aloysius Mariae L. Canete Masaya Nagaishi Tonni Agustiono Kurniawan 《Waste management (New York, N.Y.)》2014,34(6):971-979
Municipal Solid Waste Management (MSWM) is considered to be one of the most serious environmental issues in the Philippines. The annual waste generation was estimated at 10.6 million tonnes in 2012 and this is expected to double in 2025. The Republic Act (RA) No. 9003, widely known as the Ecological Solid Waste Management Act of 2000, provides the required policy framework, institutional mechanisms and mandate to the Local Government Units (LGUs) to achieve 25% waste reduction target through establishing an integrated solid waste management plan based on the 3Rs (reduce, reuse and recycling). Although the initial impact of the LGUs is still very limited in implementing the national mandate, this article highlights the successful experiences of Cebu, the second largest city in the Philippines, in reducing its MSW generation by more than 30% in the past three years. This study also explores the implementation process, innovative actions taken by the Cebu City Government in implementing the national mandate at local level and identifies the factors that influence the policy implementation. The findings suggest that the impacts of the national mandate can be achieved if the LGUs have the high degree of political commitment, planning and development of effective local strategies in a collaborative manner to meet with local conditions, partnership building with other stakeholders, capacity development, adequate financing and incentives, and in the close monitoring and evaluation of performance. 相似文献
668.
Akansha Bhatia Sangeeta Madan Jitendra Sahoo Muntjeer Ali Ranjana Pathania Absar Ahmed Kazmi 《Waste management (New York, N.Y.)》2013,33(7):1595-1601
Bacterial diversity of full scale rotary drum composter from biodegradable organic waste samples were analyzed through two different approaches, i.e., Culture dependent and independent techniques. Culture-dependent enumerations for indigenous population of bacterial isolates mainly total heterotrophic bacteria (Bacillus species, Pseudomonas species and Enterobacter species), Fecal Coliforms, Fecal Streptococci, Escherichia coli, Salmonella species and Shigella species showed reduction during the composting period. On the other hand, Culture-independent method using PCR amplification of specific 16S rRNA sequences identified the presence of Acinetobacter species, Actinobacteria species, Bacillus species, Clostridium species, Hydrogenophaga species, Butyrivibrio species, Pedobacter species, Empedobactor species and Flavobacterium species by sequences clustering in the phylogenetic tree. Furthermore, correlating physico-chemical analysis of samples with bacterial diversity revealed the bacterial communities have undergone changes, possibly linked to the variations in temperature and availability of new metabolic substrates while decomposing organics at different stages of composting. 相似文献
669.
Zsuzsanna Horváth Judit Sali Andrea Zentai Enikő Dorogházi Zsuzsa Farkas Kata Kerekes 《Journal of environmental science and health. Part. B》2013,48(3):143-152
The pesticide usages are controlled by comparing residue concentrations in treated commodities to legally permitted maximum levels (MRLs) determined based on supervised trials designed to reflect likely maximum residues occurring in practice following authorised use. The number of trials available may significantly affect the accuracy of estimated maximum residues. We conducted a study with synthetic lognormal distributions with mean of 1 and standard deviations of 0.8 and 1.0, which reflect the residue distributions observed in practice. The likely residues in samples were modelled by drawing random samples of size 3, 5, 10 and 25 from the synthetic populations. The results indicate that the estimations of highest residues (HR), used for calculation of short-term intake, and the MRLs, serving as legal limits, are very uncertain based on 3–5 trials indicated by the calculated HR0.975/HR0.025 and MRL0.975/MRL0.025 ratios of 12 and 9, and 13 and 10, respectively, which question the suitability of such trials for the intended purpose. As the 95% range of HR and MRL rapidly decreases with number of trials, ideally ≥15 but minimum 6–8 trials should be used for estimation of HR and MRL according to the current typical practice of Codex Alimentarius. 相似文献
670.
Sudha Rani Batchu Venkata R. Panditi Piero R. Gardinali 《Journal of environmental science and health. Part. B》2013,48(3):200-211
The photochemical fate of seven sulfonamides was investigated in matrices representative of natural water bodies under various light sources. Fundamental photolysis parameters such as molar absorption coefficient, quantum yield (QY) and first-order rate constants were determined. The photolysis decay rate was dependent on the protonation state of the molecule, pH of the water sample and dissolved organic matter. Natural organic matter was the most significant factor in the indirect photolysis of sulfonamides. Half-lives were in the range of minutes at 254 nm to days under natural sunlight. Under natural sunlight, all sulfonamides showed higher removal rates in natural waters implying that indirect photolysis is the predominant mechanism. 相似文献