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841.
Hongyu Liu Hu Zhang Shan Chen Dongbo Liu Hongmei Xia 《Journal of Polymers and the Environment》2006,14(4):419-426
An extracellular poly (β-hydroxybutyrate) (PHB) depolymerase was purified from a Penicillium sp. DS9701-09a by centrifugation, ultrafiltration, precipitation and gel filtration chromatography. The specific activity of the purified enzyme was 37.9-folds higher than that of the culture supernatant and the recovery yield was 11.8%. The PHB deploymerase molecular mass was 44.8 kDa from analysis of both Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Matrix-assisted laser desorption-time-of-flight (MALDI-TOF) mass spectrometer. The isoelectric point of 6.7 for the enzyme was determined by a two-dimensional electrophoresis. The optimum enzyme activity was observed at a temperature of 50 °C and pH 5.0. The apparent K
m of the enzyme was found to be 1.35 mg/mL. The PHB depolymerase consisted of 16 kinds of normal amino acids. The secondary structure of the enzyme was determined by CD spectrum. α-helix and β-turn were found to be 66% and 34% for the enzyme without ammonium sulphite. Chemical inhibition on the PHB depolymerase activity was examined and EDTA was found to have a significantly inhibitory effect. 相似文献
842.
运用主成分与聚类分析相结合的方法,根据《甘肃省城镇体系规划和城镇基础设施报告(2003)》,《中国优秀旅游城市检查标准》。甘肃省旅游局2004年对国内游客的抽样调查数据(2004),以及对甘肃省主要城市基础设施、社会经济发展等方面28项指标进行的主成分——因子聚类分析综合评价结果,得出主要城市的体系与功能评价,初步探讨甘肃省旅游城市体系,根据结果初步得出,甘肃省旅游城市体系分为,国际旅游城市——敦煌,省内一级旅游中心城市——兰州,省内二级旅游中心城市以及省内三级旅游中心城市和地方旅游中心城镇,并为各个城市的城市旅游形象和城市旅游标识体系,旅游城市的发展方向等进行了概念性规划。 相似文献
843.
Dissolved ions and suspended participates from twelve water samples from Sohag (different localities) and Aswan (behind and below the High Dam) areas were collected along the River Nile, upper Egypt, which is at present affected seriously by pressure of population, intense industrial and agricultural activities, and atmospheric fallout. Eighteen trace and nine major ions in solution and fourteen metals in suspension were analyzed to establish whether elements are of anthropogenic or natural origin. Results of this study show that the Nile water was contaminated with high concentrations of dissolved lead, and with copper, nickel and mercury to a lesser extent, suggesting that these contaminants are generated from anthropogenic activities. the other dissolved ions in the Nile are of natural origin because their metal concentrations fall within the range of the normal background and average world dissolved values for inland waters. Results show that the usual constituents of suspended particulates in the Nile seem to be some metals (Cu and Zn) adsorbed on iron and manganese oxide/hydroxide. the results also reveal that most trace and major metal particulates increase from south to north due to activities associated with the dense population living along the Nile banks. No significant differences were observed between the chemical composition of trace and major elements taken on the east and west river Nile banks. 相似文献
844.
以由地洛试剂为氧化剂,用分光光度法测定染料废水中对氨基酚的含量,进行了波长,贝特洛试剂用量及显色时间等分析条件的选择。精密度和准确度实验结果表明,该准确,快速,可用于染料废水中对氨基酚的测定。 相似文献
845.
846.
J. C. Day 《Journal of the American Water Resources Association》1972,8(6):1120-1136
Neither Canada nor the United States attach much importance to the International Joint Commission (IJC) judging by the size of staffs and annual budgets. The Commission has been restricted to a relatively minor number of functions in the Great Lakes-St. Lawrence. It has investigated: the degree and causes of water and air quality deterioration; the effects of hydroelectric and navigation projects on water levels; the impacts of water-level fluctuations; and the feasibility of a deep waterway from the St. Lawrence to the Hudson River. Projects approved by the Commission have produced less than might be expected through no fault of the Commission. The Great Lakes Fishery Commission has promoted little international management. Budgetary limitations restrict its lamprey control program; institutional limitations restrict its ability to deal effectively with fishery problems. Commission responsibilities are limited to coordination and advisory functions. Since Canada and the United States have not chosen to refer most aspects of river basin management to international bodies, an institutional void exists in the Great Lakes Basin to consider these questions on a continuous basis. There is a need for expanded international cooperation. 相似文献
847.
Ä.P. Lino Grima Susan Horton Shashi Kant 《Environment, Development and Sustainability》2003,5(3-4):297-314
Can development occur without running down natural resources in an unsustainable way? The concept of natural capital offers a way for those with divergent views (ecologists and economists for example) to discuss this difficult question. Four aspects are examined in detail. First, the role of institutions in facilitating sustainable development is discussed, with examples from forestry. Then examples from (eco)tourism illustrate the potential – and limits – of applicability of the concept. Measurement issues for natural capital are then considered in detail. Finally, the concept is applied to agricultural strategy in fragile lands, where the tradeoff between the environment and development is likely to be most severe. Some implications for future research and policy are developed. 相似文献
848.
Jeffrey Barber 《Environment, Development and Sustainability》2003,5(1-2):63-93
At the World Summit on Sustainable Development (WSSD), world leaders agreed that eliminating unsustainable production and consumption is one of the three overriding objectives of sustainable development. Achieving that objective should have been a major priority for the WSSD Plan of Implementation. Increases in consumption and production over the past decade were largely responsible for the worsening environmental and social trends. Unfortunately, the negotiators of the Plan paid insufficient attention to the lessons from 10 years of discussions about the concepts, the available policies and tools and their effectiveness, the impacts of those policies on developing countries, and the political commitment of countries in an era of globalization. Despite a promising proposal for a new ten-year work programme aimed at bridging the gap implementing the Agenda 21 commitments from Rio, Summit negotiators produced barely more than a muted echo of recommendations from the past which have yet to be taken seriously enough by the world's leaders in a comprehensive intergovernmental strategy. In the ten-year review of progress to achieve sustainable production and consumption (SPAC), governments quickly skipped past the critical work of examining why things are getting worse, avoiding the task of identifying the obstacles (which in some cases were themselves) and in turn avoiding the commitment to time-bound measurable targets. If nothing else, the WSSD demonstrated that a global strategy to achieve SPAC will come not from a UN consensus of world leaders but from a strategic alliance of responsible governments, civil society and others with a vision beyond the next election cycle. 相似文献
849.
New institutional economists have argued that there are many categories of institutions, including market and non-market institutions, which may prove economically efficient, specifically for public goods and common pool goods. The Government of India introduced a non-market community-based institution, known as Joint Forest Management (JFM), for forest management and protection in 1990. JFM is a sharing mechanism for forest planning and management based on sharing of rights and duties, control and decision-making authority over forestlands, between forest departments and local user groups. By 2001, 42 000 Village Forest Committees established under JFM were managing over 11.5 million ha forestland. These institutions have proved very useful, and have contributed to forest management as well as four aspects of sustainable human development (SHD) – ecological output, income generation, village infrastructure development, and community empowerment. In the long-term, community-based institutions will prove to be a foundation of SHD and participatory democracy. 相似文献
850.
The distribution of three important dissolved forms of nitrogen, viz. nitrate, nitrite and urea in the surface and bottom water samples collected from 27 selected hydrographic profiles, in the Arabian Sea, along the west coast of India is described. Of the three forms, nitrate concentrations were the highest and comparatively higher concentrations were observed in the bottom water. Decomposition of organic matter resulting in the release of the thermodynamically stable nitrogen species, i.e. nitrate, may be the major factor resulting in higher nitrate concentrations at these depths, where the water is also characterized by low values of dissolved oxygen and temperature. The significant positive correlation between A.O.U. and nitrate of the bottom water samples emphasizes the role of oxidative decomposition of organic matter which plays an active role in reducing the oxygen concentrations below the theoretical values since at this depth ( ≈200 m) the net production is taken to be zero. This is also evidenced by the negative correlation of nitrate with dissolved oxygen and temperature, for the bottom samples. 相似文献