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41.
随着政府加大对环保专项的资金投入,各类环保专项资金的申报管理任务日益繁重与复杂,用信息化手段辅助项目管理具有重要意义.本文通过对安徽省环保专项资金项目库系统建设的浅析,对提升全国环保专项资金管理水平具有参考与借鉴意义.结论对促进环保专项资金项目管理提供管理流程和技术参考. 相似文献
42.
为强化再生水深度脱氮除磷的能力,利用硫磺粉、海绵铁粉等制备出一种新型复合填料,并在不同HRT和C/N条件下将其与同种物质组成的颗粒混合填料进行对比实验.最后通过高通量测序技术对两填料表面的微生物种群结构进行了研究.结果表明,在不同条件下新型填料的脱氮除磷能力均优于颗粒混合填料;当HRT=4 h、C/N=1时,新型填料的总氮、总磷去除率均分别比颗粒填料高出30%以上.根据高通量测序结果,两反应器内的反硝化体系均由硫自养反硝化种群和异养反硝化种群构成,且新型填料系统内的硫自养反硝化菌群所占比例更大,两反应器内的优势种属分别为Sulfurimonas和Acinetobacter. 相似文献
43.
张军亮 《中国环境管理干部学院学报》2011,21(2):85-88
"公共图书馆虚拟信息咨询服务"是俄罗斯专门信息咨询性网站提供的项目,它具有全国图书馆的合作性,咨询服务对象和咨询内容具有公众性。揭示和探讨虚拟咨询服务模式的形成、服务内容、服务特色与服务方式,以全面展现俄罗斯虚拟咨询服务的整体发展状况和水平,在此基础上,分析和总结俄罗斯虚拟咨询服务的6个特点。 相似文献
44.
秦皇岛近海养殖对潮间带微生物群落多样性的影响 总被引:1,自引:0,他引:1
潮间带微生物群落在驱动海岸带生态系统物质循环和能量流动中具有重要作用,近海养殖造成的环境问题日益凸显,但其对潮间带微生物群落结构的影响还缺乏研究。采用变性梯度凝胶电泳(DGGE)和限制性片段长度多态性(RFLP)的分子生物学技术,研究秦皇岛养殖区与旅游区潮间带沉积物中微生物多样性的差异,分析养殖区微生物的16S rRNA基因文库的组成特征。结果表明:养殖区的微生物群落结构与旅游区形成较大的差异,DGGE图谱中养殖区的特有条带主要集中于γ-变形菌纲(γ-proteobacteria),还分布于α-变形菌纲(α-proteobacteria),拟杆菌门(Bacteroidetes),放线菌门(Actinobacteria)和厚壁菌门(Firmicutes)。影响潮间带微生物的群落结构的主要环境因子包括温度、盐度、pH和NO3-浓度,影响率达55.2%。对差异最大的洋河大桥南养殖区(Q1站)的微生物样品建立克隆文库分析群落结构,变形菌门(Proteobacteria)为优势菌群,占总群落的60%,其中γ-变形菌纲是主要存在的微生物纲,其余菌群包括放线菌门、拟杆菌门、蓝藻菌门(Cyanobacteria)和疣微菌门(Verrucomicrobia)的微生物。养殖区海岸带微生物群落中出现了与环境污染和赤潮密切相关的菌群,如拟杆菌门、肠杆菌属(Enterobacteriaceae)和α-变形细菌红细菌目(Roseovarius)的微生物。 相似文献
45.
46.
Assessing the impact of fungicide enostroburin application on bacterial
community in wheat phyllosphere 总被引:1,自引:0,他引:1
Likun Gu Zhihui Bai Bo Jin Qing Hu Huili Wang Guoqiang Zhuang Hongxun Zhang 《环境科学学报(英文版)》2010,22(1):134-141
Fungicides have been used extensively for controlling fungal pathogens of plants. However, little is known regarding the effects that fungicides upon the indigenous bacterial communities within the plant phyllosphere. The aims of this study were to assess the impact of fungicide enostroburin upon bacterial communities in wheat phyllosphere. Culture-independent methodologies of 16S rDNA clone library and 16S rDNA directed polymerase chain reaction with denaturing gradient gel electrophoresis (PCR-DGGE) were used for monitoring the change of bacterial community. The 16S rDNA clone library and PCR-DGGE analysis both confirmed the microbial community of wheat plant phyllosphere were predominantly of the γ-Proteobacteria phyla. Results from PCR-DGGE analysis indicated a significant change in bacterial community structure within the phyllosphere following fungicide enostroburin application. Bands sequenced within control cultures were predominantly of Pseudomonas genus, but those bands sequenced in the treated samples were predominantly strains of Pantoea genus and Pseudomonas genus. Of interest was the appearance of two DGGE bands following fungicide treatment, one of which had sequence similarities (98%) to Pantoea sp. which might be a competitor of plant pathogens. This study revealed the wheat phyllosphere bacterial community composition and a shift in the bacterial community following fungicide enostroburin application. 相似文献
47.
Analytical solutions of three-dimensional contaminant transport in uniform flow field in porous media: A library 总被引:1,自引:0,他引:1
Hongtao Wang Huayong Wu 《Frontiers of Environmental Science & Engineering in China》2009,3(1):112-128
The purpose of this study is to present a library of analytical solutions for the three-dimensional contaminant transport
in uniform flow field in porous media with the first-order decay, linear sorption, and zero-order production. The library
is constructed using Green’s function method (GFM) in combination with available solutions. The library covers a wide range
of solutions for various conditions. The aquifer can be vertically finite, semi-infinitive or infinitive, and laterally semi-infinitive
or infinitive. The geometry of the sources can be of point, line, plane or volumetric body; and the source release can be
continuous, instantaneous, or by following a given function over time. Dimensionless forms of the solutions are also proposed.
A computer code FlowCAS is developed to calculate the solutions. Calculated results demonstrate the correctness of the presented
solutions. The library is widely applicable to solve contaminant transport problems of one- or multiple- dimensions in uniform
flow fields. 相似文献
48.
曹雯 《防灾科技学院学报》2009,11(2):105-107
图书馆是人们获取知识信息的公共场所,它的安全也应该引起我们的重视.本文从编制图书馆灾害应急预案、建立健全各种规章制度、加强对图书馆工作人员应对灾害能力的培训、健全各种应急设备、数字馆藏容灾制度等几个方面论述了图书馆防灾减灾的方法与手段. 相似文献
49.
Juanli Yun Anzhou M Yaoming Li Guoqiang Zhuang Yanfen Wang Hongxun Zhang 《环境科学学报(英文版)》2010,22(8):1232-1238
Zoige wetland is one of the most important methane emission centers in China. The oxidation of methane in the wetland a ects
global warming, soil ecology and atmospheric chemistry. Despite their global significance, microorganisms that consume methane in
Zoige wetland remain poorly characterized. In this study, we investigated methanotrophs diversity in soil samples from both anaerobic
site and aerobic site in Zoige wetland using pmoA gene as a molecular marker. The cloning library was constructed according to
the pmoA sequences detected. Four clusters of methanotrophs were detected. The phylogenetic tree showed that all four clusters
detected were a liated to type I methanotrophs. Two novel clusters (cluster 1, cluster 2) were found to relate to none of the recognized
genera of methanotrophs. These clusters have no cultured representatives and reveal an ecological adaptation of particular uncultured
methanotrophs in Zoige wetland. Two clusters were belonging to Methylobacter and Methylococcus separately. Denaturing gradient
gel electrophoresis gel bands pattern retrieved from these two samples revealed that the community compositions of anaerobic soil and
aerobic soil were di erent from each other while anaerobic soil showed a higher metanotrophs diversity. Real-time PCR assays of the
two samples demonstrated that aerobic soil sample in Zoige wetland was 1.5 times as much copy numbers as anaerobic soil. These data
illustrated that methanotrophs are a group of microorganisms influence the methane consumption in Zoige wetland. 相似文献
50.
随着沿岸水体氮素富营养化的加剧,生物脱氮作用越来越受重视,位于海陆交界的湿地红树林生态系统作为一个自然脱氮体系备受关注.本研究以典型亚热带湿地红树林(香港Mai Po)作为对象,结合传统的富集筛选和分子生物学方法--建立nosZ基因克隆文库和RFLP分析技术对红树林沉积物中反硝化细菌脱氮能力、种群结构和丰度进行研究.从Mai Po红树林沉积物中共筛选到12株好氧反硝化菌和8株厌氧反硝化菌,其中好氧反硝化菌包括Pseudomonas(4株)、Comamonas(2株)和Acinetobacter(2株)等7个菌属,厌氧反硝化菌属于Pseudomonas、Agrobacterium和Uncultured Betaproteobacteria bacterium(2株)等7个菌属.筛选到的好氧和厌氧反硝化菌均具有较高的脱氮能力,大部分在2d内NO3--N去除率达到98%以上.建立Mai Po红树林湿地反硝化细菌的nosZ基因克隆文库的结果表明,反硝化细菌的50个克隆子中有26个克隆子分属于11个未知类群,其余克隆子属于Pennisetum类群(26%),β-proteobacterium类群(10%),Entandrophragma类群(4%),Pseudomonas类群(4%)和denitrifying bacterium类群(4%).可见Mai Po红树林中反硝化细菌具有很高的生物多样性. 相似文献