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11.
DNA链断裂作为醛类污染物接触标志物的研究 总被引:2,自引:0,他引:2
应用单细胞凝胶电泳技术 ,通过对小鼠脾淋巴细胞染毒试验 ,测定了甲醛、乙醛、丙稀醛单独及联合作用下对细胞DNA分子链断裂损伤。结果表明 ,3种醛均能引起DNA分子发生链断裂 ,对于乙醛和丙稀醛存在明确的剂量 -反应关系 ,3种化合物还还存在着一定的协同作用。上述结果提示醛类污染物潜在致癌性的一个可能机制以及DNA断裂作为醛类化合物接触标志物的可能性。 相似文献
12.
采用自然吸入的暴露方法,通过分析肺灌洗液成分及脾脏淋巴细胞的转化功能,观察碳素厂冷压车间空气对大鼠肺损伤及免疫功能的影响,结果表明,在自然吸入染毒期间,引起大鼠肺灌洗液中乳酸氢酶、酸性磷酸酶和唾液含量或高,肺巨噬细胞及其Fc受体数降低,并且还引起脾脏淋巴细胞的转化能力下降。 相似文献
13.
As a preliminary step to preimplantation diagnosis of sickle cell disease in unfertilized eggs or 8-cell embryos of heterozygous parents, we established quality control for detection of the mutant and normal alleles of the beta-haemoglobin gene using single buccal cells. Efficient polymerase chain reaction (PCR) amplification of a 680 base pair sequence of the beta-globin gene spanning the site of the sickle cell mutation was obtained for 79 per cent of single heterozygous cells. In 71 per cent of cases, both alleles were detected. With this current efficiency, we predict that a clinical preimplantation diagnosis at the 8-cell embryo stage could be carried out safely and reliably for a couple at risk of transmitting sickle cell disease to their children. 相似文献
14.
A protocol for easy storage and later expansion of lymphocyte populations is given. Compared with methods using transformed cell lines, the method has a number of advantages for repeated production of cells for the isolation of DNA in amounts sufficient for use in diagnostic DNA technology. 相似文献
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16.
酸沉降破坏材料造成的经济损失的估算研究 总被引:3,自引:1,他引:3
酸沉降对材料的破坏和其经济损失估算是国际上酸沉降研究领域听一个重要课题,国内在经方面的研究较少,文章通过对“两广酸沉降破坏材料造成的经济损失的估算”研究,提出了了一套较实用的经济损失估算方法。 相似文献
17.
I. Shagina H. L. Dadali V. P. Sitnikov V. V. Pugachev N. A. Malygina O. V. Evgrafov 《黑龙江环境通报》1995,15(1):27-34
Ninety-two families with spinal muscular atrophy (SMA) applied for genetic counselling and further prenatal diagnosis. To minimize expenses, only one tightly linked informative marker was determined in the course of preliminary examination, and non-radioactive allele detection was preferably used. Four prenatal diagnoses of SMA type I, four of SMA type II, and one of SMA type III were made. This trial programme shows the considerable requirements, importance, and potential effectiveness of prenatal prediction of SMA in Russia. 相似文献
18.
In vitro characteristics of human fetal cells have been investigated after chorionic villus sampling at the first trimester and amniocentesis at the second trimester of pregnancy. Light microscopy revealed heterogeneous morphology of cell types in both the chorionic villus culture and the amniotic fluid cultures. Based on the experiments performed, chorionic villus cells are more sensitive to pronase, trypsin, and versene during subculture and have a higher DNA content per single cell and release more [125I]-Beta-human chorionic gonadotropin into culture medium than those found in amniotic fluid cells. The practical applications of this study are discussed. 相似文献
19.
宁夏煤田火灾的危害及综合治理研究 总被引:2,自引:0,他引:2
针对宁夏回族自治区煤田火灾的状况,指出了宁夏煤田火灾所造成的环境、地质及社会灾害,并根据宁夏煤田火灾的情况,进行了系统的分析,对灭火方法进行了研究和探讨。 相似文献
20.
Mr. M. T. Rebello G. Hackett J. Smith F. E. Loeffler S. Robson N. Maclachlan R. W. Beard C. H. Rodeck R. Williamson D. V. Coleman C. Williams 《黑龙江环境通报》1991,11(1):41-46
Ten-ml samples of amniotic fluid were taken from pregnancies being terminated at 8–14 weeks' gestation. DNA was extracted from the amniotic cells by sequential centrifugation and analysed using the polymerase chain reaction (PCR). Fifteen samples were analysed for evidence of maternal contamination using Mfd5 oligo-nucleotide primers for repeat polymorphisms. Ten amniotic fluid samples were tested for the Delta-F508 deletion characteristic of cystic fibrosis to demonstrate a diagnostic application for the technique. In each case, DNA extracted from fetal tissue from the same pregnancy was included in the controls. In 14 of the 15 cases tested with the Mfd5 primers, both the amniotic fluid DNA and the fetal DNA showed no evidence of contaminating DNA. In one case, neither the amniotic fluid cells nor the fetal cells yielded results. In nine of the ten cases tested with the Delta-F508 primers, the amniotic fluid cell DNA provided accurate information about the genetic status of the fetus; in the tenth, the fetal DNA failed to amplify. The results indicate that adequate DNA can be extracted from amniotic fluid from 8 weeks' gestation onward and these samples are suitable for prenatal diagnosis using PCR. 相似文献