An integrative analysis of miRNA and mRNA expression in the brains of Alzheimer's disease transgenic mice after real-world PM2.5 exposure

Fine particulatematter (PM2.5) is associated with increased risks of Alzheimer’s disease (AD),yet the toxicologicalmechanisms of PM2.5 promoting AD remain unclear. In this study,wildtype and APP/PS1 transgenic mice (AD mice) were exposed to either filtered air (FA) or PM2.5 for eight weeks with a real-world exposure system in Taiyuan, China (mean PM2.5 concentration in the cage was 61 μg/m3). We found that PM2.5 exposure could remarkably aggravate AD mice’s ethological and brain ultrastructural damage, along with the elevation of the pro-inflammatory cytokines (IL-6 and TNF-α), Aβ-42 and AChE levels and the decline of ChAT levels in the brains. Based on high-throughput sequencing results, some differentially expressed (DE) mRNAs and DE miRNAs in the brains of AD mice after PM2.5 exposure were screened.Using RT-qPCR, seven DEmiRNAs (mmu-miR-193b-5p, 122b-5p, 466h-3p, 10b-5p, 1895, 384–5p, and 6412) and six genes (Pcdhgb8, Unc13b, Robo3, Prph, Pter, and Tbata) were evidenced the and verified. Two miRNA-target gene pairs (miR-125b-Pcdhgb8 pair and miR-466h-3p-IL-17Rα/TGF-βR2/Aβ-42/AChE pairs) were demonstrated that they were more related to PM2.5-induced brain injury. Results of Gene Ontology (GO) pathways and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways predicted that synaptic and postsynaptic regulation, axon guidance, Wnt, MAPK, and mTOR pathways might be the possible regulatory mechanisms associated with pathological response. These revealed that PM2.5- elevated pro-inflammatory cytokine levels and PM2.5-altered neurotransmitter levels in AD mice could be the important causes of brain damage and proposed the promising miRNA andmRNA biomarkers and potentialmiRNA-mRNA interaction networks of PM2.5-promoted AD.     

河南省汛期极端降水事件分析

利用河南1961～2006年50个气象站台站汛期（6～8月份）逐日降水量资料，定义95%降水分位数为极端降水事件的阈值，建立不同站近46年汛期极端降水事件发生频次的时间序列。在此基础上采用趋势分析、最大熵谱分析等统计技术方法，对河南降水事件发生频次的空间分布及年际变化特点进行了分析。结果表明：空间变化上总体具有北多南少的特点，而且汛期降水量的比重与极端降水事件发生频次的高低存在着很好的一致性；空间分布上主要有全省一致型、西北 东南型、南阳盆地型和中部分布型等4种类型，其中全省一致分布型为最主要的空间模态；年际变化趋势各地有所不同，豫西、豫南区为减少趋势，而豫中、豫北、豫东和豫西南区表现为增加趋势，而且在振荡形态上各有同异，以2~8年和10年左右的年代际变化最为普遍。     

乌江流域梯级水库入出库河流中总汞和甲基汞的时空分布

2006年1~12月，每月采集乌江流域梯级水库入出库河流水样，用两次金汞齐-冷原子荧光光谱法和蒸馏-乙基化结合GC CVAFS法测定了水中总汞和甲基汞的浓度。结果表明：（1）入出库河流中总汞年均加权浓度分别为317和 2.34 ng/L，甲基汞为014和 0.18 ng/L。（2）不同水库入出库河流中总汞和甲基汞的时空分布特征不同，位于上游第一级的普定和洪家渡水库入库河流中总汞有明显的季节变化趋势，且显著低于出库河流；而甲基汞的季节变化在出库河流中较为明显，而且库龄大的普定、东风、乌江渡水库出库河流中甲基汞浓度显著高于入库河流。（3）相关分析发现水库入库河流中总汞、甲基汞浓度主要受悬浮颗粒物的影响，而与水量间的相关性因水库所处位置的不同而有差异，上游的普定和洪家渡水库中呈显著正相关，其它水库中呈负相关。     

鄱阳湖典型湿地土壤-植物系统重金属沿湖向富集及迁移转换特征分析

特殊的水文节律导致鄱阳湖形成面积较大的周期性环湖消落带,大量的营养物质和污染物极易在消落带中发生沉积,选择鄱阳湖典型河口型消落带,沿湖向按照不同植被类型设置采样断面,采集土壤-沉积物和优势植物样品(不同组织),分析不同时空下土壤/沉积物和优势植物体中重金属(Cr、 Ni、 Cu、 Zn、 As、 Cd、 Sb和Pb)的含量特征及富集水平,并进一步分析土壤-植物系统中重金属的迁移转换特征,揭示鄱阳湖典型消落带土壤-植物系统重金属迁移转换的影响因素.结果表明,消落带土壤/沉积物重金属含量沿湖向表现为明显的分布规律,呈现为先升高后降低的分布特征,即季节性淹水带土壤/沉积物是重金属富集的主要地带,Cu、 Pb和Sb在土壤/沉积物中的富集水平较高,其中Cu和Sb为显著富集(EF>5);潜在生态风险结果表明季节性淹水带总体处于轻度生态危害水平(70≤RI<140),显著高于淹水带和未淹水带.消落带沿湖向带状分布的优势植物体中重金属含量并未表现出显著的空间分布特征,但表现为显著的季节差异,生长季(4月)优势植物体内重金属具有较高的含量水平,重金属不同介质和组织的分配大体遵循基质>根...     

Characterizing a Major Urban Stream Restoration Project: Nine Mile Run (Pittsburgh,Pennsylvania, USA)

Urban stream restoration continues to be used as an ecological management tool, despite uncertainty about the long‐term sustainability and resilience of restored systems. Evaluations of restoration success often focus on specific instream indicators, with limited attention to the wider basin or parallel hydrologic and geomorphic process. A comprehensive understanding of urban stream restoration progress is particularly important for comparisons with nonurban sites as urban streams can provide substantial secondary benefits to urban residents. Here, we utilize a wide range of indicators to retrospectively examine the restoration of Nine Mile Run, a multi‐million dollar stream restoration project in eastern Pittsburgh (Pennsylvania, USA). Examination of available continuous hydrological data illustrates the high cost of failures to incorporate the data into planning and adaptive management. For example, persistent extreme flows drive geomorphic degradation threatening to reverse hydrologic connections created by the restoration and impact the improved instream biotic communities. In addition, human activities associated with restoration efforts suggest a positive feedback as the stream restoration has focused effort on the basin beyond the reach. Ultimately, urban stream restoration remains a potentially useful management tool, but continued improvements in post‐project assessment should include examination of a wider range of indicators.     

Does Riparian Forest Restoration Thinning Enhance Biodiversity? The Ecological Importance of Large Wood

Intact riparian ecosystems are rich in biological diversity, but throughout the world, many have been degraded. Biodiversity declines, particularly of vertebrates, have led to experimental efforts to restore riparian forests by thinning young stands to accelerate creation of large diameter live trees. However, many vertebrates depend on large diameter deadwood that is standing as snags or fallen to the forest floor or fallen into streams. Therefore, we reviewed the sizes of deadwood and live trees used by different vertebrate species to understand which species are likely to benefit from different thinning treatments. We then examined how riparian thinning affects the long‐term development of both large diameter live trees and deadwood. To this end, we used a forest growth model to examine how different forest thinning intensities might affect the long‐term production and abundance of live trees and deadwood. Our results suggest that there are long‐term habitat tradeoffs associated with different thinning intensities. Species that utilize large diameter live trees will benefit most from heavy thinning, whereas species that utilize large diameter deadwood will benefit most from light or no thinning. Because far more vertebrate species utilize large deadwood rather than large live trees, allowing riparian forests to naturally develop may result in the most rapid and sustained development of structural features important to most terrestrial and aquatic vertebrates.     

基于GB/T 29309标准的HALT测试技术分析

本文以标准GB/T 29309-2012为基础,以某款开关电源为例,对HALT试验设备、工作极限与破坏极限、试验步骤及设备运行中注意点等方面展开介绍,为电子产品可靠性测试提供相关探讨。     

捕集阱顶空-气相色谱/质谱法检测地表水中25种挥发性有机物

建立了捕集阱顶空-气相色谱/质谱法检测地表水中25种挥发性有机物的分析方法。采用正交实验设计对捕集阱顶空条件进行了优化,该方法相关系数0.995,加标回收率为90%~110%,方法检出限为0.08~0.39μg/L,相对标准偏差(n=7)为0.7%~4.8%,仪器检出限低于0.04μg/L。方法准确度和灵敏度较好,可以满足对地表水中25种挥发性有机物的检测要求。     

Interactions Among the Copper Species and Forms in Sea Water/Sediments and Copper Bioaccumulation in Oysters

Abstract

Earlier papers indicated that the first incident of green discoloration in oysters (Crassostrea gigas) and the mass mortality observed in 1986 along the Taiwan Erhjin Chi coastal area were caused by the higher contents of total copper and copper species (mainly bioavailable and free ion) in sea water. the copper in sea water would be sorbed by suspended matter and transferred to sediments, and the copper in the sediments would also be desorbed to sea water. Processes of copper adsorption and desorption are the major factors influencing the contents of total copper and copper species in sea water and sediments. in this study, the Erhjin Chi sediments were mixed with sea water by a shaker technique. When the mixture was shaken for one hour, analogous to tidal mixing in estuaries, only copper desorption from sediments was observed. If the shaking time is increased for more than 3 hours, the copper released from the sediments was resorbed to the remaining solid phases. the higher the contents of mud (91.71%) and total copper (701 mg kg^?1) in sediments, the higher the copper desorption rate (1.86 ppm hr^?1) and copper adsorption rate (0.50 ppm hr^?1) were observed. in sediments containing lower mud (0.80%) and lower copper (43.5 mg kg^?1), the copper desorption and adsorption rates were 0.83 ppm hr^?1 and 0.22 ppm hr^?1, respectively. the interactions among the total copper and copper species in sea water and sediments, chemical and ecological parameters, and copper bioaccumulation in oysters in the Erhjin Chi estuarine and coastal area are also discussed.     

A Phenomenological Perspective on Ethical Duty in Environmental Communication

This article examines the framing of environmental risks and natural disasters in factual entertainment television programs of the early 2000s, a hybrid form combining techniques from documentary with techniques such as dramatic reconstructions and computer-generated imagery from entertainment genres. Using qualitative frame analysis, it examines a range of factual entertainment television programs' framing of environmental risk and natural disasters in terms of their attitudes, representation of human participants and visual composition. The article considers the similarities and differences in the framing of natural disasters as factual entertainment compared to the framing of natural disasters in news, documentary and fiction film. It argues that such programs offer representational frames both consonant with and distinct from other media and concludes that they problematically offer a predominantly fatalistic response to environmental risk, constructing natural disasters as voyeuristic spectacles for vicarious entertainment.