荧光法快速测定浮游植物以及应用于梅梁湖预警监测可行性探讨

YSI 6600-V2多功能水质自动监测仪采用荧光原理快速测定水体中浮游植物蓝绿藻的密度,梅梁湖夏季水华高发期时,湖体中的浮游植物几乎全部是蓝绿藻。传统的浮游植物监测前处理方面就需要24~48 h,最终要数天后才有镜检结果,导致浮游植物数据滞后,不能很好的应用到预警监测中。通过比对相关性验证,保证荧光法数据的可信度,提高工作效率,为预警监测提供有力的支持。     

Prenatal diagnosis of congenital toxoplasmosis

Ninety-three pregnant women with Toxoplasma gondii seroconversion during pregnancy underwent prenatal diagnosis of fetal toxoplasmosis. The following tests were used: (1) amniocentesis for mouse inoculation (93 subjects), (2) amplification of T. gondii DNA by polymerase chain reaction (PCR) (79 subjects), and (3) cordocentesis for the detection of T. gondii-specific IgM antibodies (13 subjects). All patients had serial ultrasonographic scans to detect those fetuses with abnormalities that could be associated with congenital toxoplasmosis. Eighteen pregnancies (19.4%) had evidence of vertical transmission. A total of 11/18 (61.1%) had positive amniotic mouse inoculation test, while 10/12 (83.3%) had positive PCR results. The combination of both tests allowed the prenatal diagnosis in 17/18 infected fetuses (94.4%). All patients who underwent cordocentesis for the detection of T. gondii-specific IgM antibodies had negative results. However, in two of the above cases fetal toxoplasmosis was detected by amniotic fluid studies. In five of the infected fetuses there were abnormal ultrasonographic findings. All pregnancies with evidence of vertical transmission were terminated, whereas the remaining pregnancies proceeded normally to term. The present data showed that amniotic fluid studies, preferably PCR amplification of T. gondii DNA, are the best diagnostic tools for the detection of vertical transmission in pregnancies with seroconversion during pregnancy. Copyright © 2002 John Wiley & Sons, Ltd.     

PVC电缆火灾早期特征气体的组成分析和传感器探测

利用气体传感器对电缆绝缘过热释放的气体进行监测有望实现电气火灾早期预警,目前该技术的发展由于特征气体未得到确认而受到限制。分析了聚氯乙烯(PVC)电缆绝缘层在不同温度下释放的气体组成,并对其主要成分进行传感器测试。TG-IR和GC-MS分析表明PVC电缆绝缘层在200℃前已出现微小失重,释放出以邻苯二甲酸二辛酯(DOP)等塑化剂为主的气体。气敏测试中,商用半导体气体传感器对DOP和电缆蒸气产生较好、相似的响应性能。因此,DOP可以作为PVC电缆火灾早期的特征气体。     

Early amniocentesis—a cytogenetic evaluation of over 1500 cases

We report our cytogenetic experience of 1554 early amniocenteses between weeks 11 and 14 of gestation, of which 44 per cent were performed prior to week 14. The mean culture time was 14·5 days. Karyotyping was successful in 99·7 per cent of cases. In 9·9 per cent of cases, there was pseudomosaicism with a high rate of loss of an X-chromosome and structural aberration of chromosome 1, which may be due to the Chang medium. The mosaic rate was 0·5 per cent. The overall aberration rate was 2·8 per cent. Our data confirm the reliability of early amniocentesis, which is a serious alternative to standard amniocentesis and chorionic villus sampling (CVS).     

浙江墩头盆地横山组孢粉化石的发现及其地层意义

浙江墩头盆地红色岩层中上部首获丰富的以Cassopllis为主(58.39—62.54％)伴有Exesipollenites(15.21—15.84％),Hsuisporites(0.56—0.62％),Cicatricosisporites(0—0.31％)等孢粉化石。该孢粉组合特征既与国内外晚侏罗世孢粉组合面貌差别较大,又与早白垩世中晚期孢粉组合面貌存在明显差异,显示了早白垩世早期孢粉组合的色彩。笔者认为这套红色岩层应归早白垩世早期横山组为宜。