废弃荧光灯无害化、资源化处置研究进展

阐述了废弃荧光灯回收利用的潜在意义，介绍了国内外废弃荧光灯资源回收与处置方法。采用回收利用法处理废弃荧光灯，其中包括荧光灯的破碎与物理分离，汞的无害化处理，以及稀土三基色荧光粉的回收和分离。根据稀土三基色荧光粉的特点，目前国外主要采用超临界CO2萃取法、湿法冶金法、风力分选法、离心法和浮选法分离回收稀土，对循环利用稀土资源具有一定的意义。     

Phenotypic analysis of fetal blood leucocytes: Potential for prenatal diagnosis of immunodeficiency disorders

Recent technological advances allow the detection and quantitation of subsets of leucocytes using monoclonal antibodies. We have taken advantage of this to study the ontogeny of fetal blood leucocytes, using very small blood samples obtained at fetoscopy. By 14 weeks gestation T cells represent 35 per cent or more of fetal leucocytes and the distribution of the helper/inducer and suppressor/cytotoxic subsets is similar to that of adults. B lymphocytes before 161/2 weeks are low (4–20 per cent), but rise to a mean of 28 per cent in 17–26 week fetuses. Granulocytic cells, many of which are phenotypically immature, represent 18–34 per cent of total leucocytes. The methodology employed is very reliable and offers the opportunity for the prenatal diagnosis of some immunodeficiency disorders, since using the same reagents we have diagnosed children with severe combined immunodeficiency shortly after birth.     

Limited use of chromosomal markers in prenatal diagnosis

By aid of fluorescent centromere markers in chromosome No. 3 it could be shown that crossing over in a translocation quadrivalent had occurred on two occasions in a father who is carrier of a 3/5 translocation. This case demonstrates that marker chromosomes cannot always be used to trace the parental origin of structurally abnormal chromosomes.     

应用绿色荧光蛋白基因标记细菌进行生物膜结构定量化新方法

绿色荧光蛋白基因pEGFP经CaCl₂转化法标记E.coli JM109菌株,获得的标记菌株作为模式细菌接种含50μg/mL氨苄的LB培养基,在摇瓶中与火山岩颗粒共混培养挂膜(37℃,120r/min,16h).用激光共聚焦扫描显微镜摄取获得火山岩填料生物膜250μm×250μm区域不同层面的图片堆,所获图片堆经COMSTAT程序处理可以获得相关的定量化参数,如16h生物膜平均厚度为0.120844μm,生物膜最大厚度为10.5μm,生物膜体积为0.136986μm³/μm²,生物膜表面积21338.1μm²,生物膜比表面积为3.36854μm²/μm³.该方法也可以扩展至其他绿色荧光蛋白基因标记细菌的生物膜结构定量化.     

荧光定量PCR技术在环境领域的应用

实时荧光定量PCR技术作为一项新兴技术,越来越受到人们的重视,它以快速、准确定量、便捷等优点广泛应用于科学研究各个领域。近年来,实时荧光定量PCR技术不断发展,并应用于环境领域中,大大提高了环境监测水平。综述了实时荧光定量PCR技术的基本原理及其在国内外环境领域中的应用。