Characteristics of sewer biofilms in aerobic rural small diameter gravity sewers

Small diameter gravity sewers(SDGS) are extensively used to collect rural sewage as they are low in cost and quick to construct.However,the characteristics of biofilms in rural SDGS are still not clear.In this study,biofilms characteristics of aerobic rural SDGS were investigated using simulations in a lab under different flow conditions and slopes.Results indic ated that the average thickness of aerobic rural SDGS biofilms was in the range of 350-650 μm,decreasing at locations with variable flow and high slopes.Protein was the most abundant substance in extracellular polymeric substance of SDGS biofilms.The most abundant bacteria,Proteobacteria,Actinobacteria,and Bacteroidetes,and functional bacteria showed different distributions when analyzed through Illumina HiSeq sequencing of 16 S rRNA.The relative abundances of denitrifying bacteria,nitrite-oxidizing bacteria,and sulfate-reducing bacteria(SRB) were lower during variable flow than during stable flow.High slopes(15‰) decreased SRB presence,which could be used to mitigate H_2 S accumulation in aerobic SDGS.Overall,this study describes the characteristics of aerobic rural SDGS biofilms and provides valuable suggestions for the optimal design of SDGS based on these characteristics.     

Characteristics of microbial community functional structure of a biological coking wastewater treatment system

Nitrogenous heterocyclic compounds are key pollutants in coking wastewater; however, the functional potential of microbial communities for biodegradation of such contaminants during biological treatment is still elusive. Herein, a high throughput functional gene array (GeoChip 5.0) in combination with Illumina HiSeq2500 sequencing was used to compare and characterize the microbial community functional structure in a long run (500 days) bench scale bioreactor treating coking wastewater, with a control system treating synthetic wastewater. Despite the inhibitory toxic pollutants, GeoChip 5.0 detected almost all key functional gene (average 61,940 genes) categories in the coking wastewater sludge. With higher abundance, aromatic ring cleavage dioxygenase genes including multi ring1,2diox; one ring2,3diox; catechol represented significant functional potential for degradation of aromatic pollutants which was further confirmed by Illumina HiSeq2500 analysis results. Response ratio analysis revealed that three nitrogenous compound degrading genes- nbzA (nitro-aromatics), tdnB (aniline), and scnABC (thiocyanate) were unique for coking wastewater treatment, which might be strong cause to increase ammonia level during the aerobic process. Additionally, HiSeq2500 elucidated carbozole and isoquinoline degradation genes in the system. These findings expanded our understanding on functional potential of microbial communities to remove organic nitrogenous pollutants; hence it will be useful in optimization strategies for biological treatment of coking wastewater.     

Colony-level selection effects on individual and colony foraging task performance in honeybees, Apis mellifera L.

In honeybees, as in other highly eusocial species, tasks are performed by individual workers, but selection for worker task phenotypes occurs at the colony level. We investigated the effect of colony-level selection for pollen storage levels on the foraging behavior of individual honeybee foragers to determine (1) the relationship between genotype and phenotypic expression of foraging traits at the individual level and (2) how genetically based variation in worker task phenotype is integrated into colony task organization. We placed workers from lines selected at the colony level for high or low pollen stores together with hybrid workers into a common hive environment with controlled access to resources. Workers from the selected lines showed reciprocal variation in pollen and nectar collection. High-pollen-line foragers collected pollen preferentially, and low- pollen-line workers collected nectar, indicating that the two tasks covary genetically. Hybrid workers were not intermediate in phenotype, but instead showed directional dominance for nectar collection. We monitored the responses of workers from the selected strains to changes in internal (colony) and external (resource) stimulus levels for pollen foraging to measure the interaction between genotypic variation in foraging behavior and stimulus environment. Under low-stimulus conditions, the foraging group was over-represented by high-pollen-line workers. However, the evenness in distribution of the focal genetic groups increased as foraging stimuli increased. These data are consistent with a model where task choice is a consequence of genetically based response thresholds, and where genotypic diversity allows colony flexibility by providing a range of stimulus thresholds. Received: 3 May 1999 / Received in revised form: 22 December 1999 / Accepted: 23 January 2000     

Review on the study of mercury/silver ions-pyrimidine base pairs in DNA duplexes and their application in detection technology北大核心CSCD

Nucleic acids and their analogues are getting more and more attention. Metal-mediated base pairs as a kind of simple and functionalized nucleic acids in special positions have widened the scope of application of functional nucleic acids and their analogues. In this type of base pairs, the representative is the interaction between metal ions and pyrimidine bases, especially the research on thymine-Hg2+-thymine (T-Hg2+-T) and cytosine-Ag+-cytosine (C-Ag+-C) base pairs. This review summarizes the structure and mechanism of metal-mediate pyrimidine base pairs as well as the application in the biochemical analysis. It explores the mode and ratio of coordination between metal ions and base pairs, the effects on the stability of DNA helical structure, the related crystal structure and the three-dimensional configuration information in the DNA helix. The analytical application mainly includes various probes of metal ions, small molecules, protein and the detection of single nucleotide polymorphism. Among them the most widely used metal ions detection, and its combination with spectrum technology, visualization and amplification technology greatly promoted the rapid development of sensitive detection technology. In further studies, it is necessary to reveal the mechanism of interaction between T-Hg2+-T and C-Ag+-C, and more attention should be paid to combining of T-Hg2+-T and C-Ag+-C base pairs with other new technologies. The scope of practical application should also be further extended.     

Evolution of extreme polyandry in the honeybee Apis mellifera L.

A number of hypotheses have been proposed to explain the evolution of multiple mating in the honeybee queen. In particular, the consequences of reduced intracolonial relatedness provide plausible explanations for multiple mating with up to ten drones, but fail to account for the much higher mating frequencies observed in nature. In this paper, we propose an alternative mechanism which builds on non-linear relationships between intracolonial frequencies in genotypic worker specialization and colony fitness. If genes for any worker specialization confer an advantage on colony fitness only when they are rare, this would require a stable mix of sperm from a few drones which contribute that trait, and many which do not. To ensure both specific, low within-colony proportions of “rare specialist” genes, and to reduce random variation of these proportions would require mating with high numbers of drones. The quantitative implementation shows that moderate to very high numbers of matings are required to exploit colony advantages from genotypic allocation of workers to rare tasks. Extreme polyandry thus could result from colony selection dependent on the intracolonial frequency of rare genetic specialists. Received: 30 January 1998 / Accepted after revision: 7 October 1998     

MODIFIED WATER QUANTITY RECEIVING MODEL FOR FLORIDA CONSERVATION AREAS1

ABSTRACT: The Conservation Areas in South Florida have been considered as one of the major water storage areas to provide a water supply for the Everglades National Park and Lower East Coast (LEC). Due to the increasing water demands of the area, additional backpumping of the surplus runoff from the LEC area into the Conservation Areas has been considered as one of several alternative plans. The Receiving Water Quantity (EPA, 1971) model has been adapted and modified to be applicable in the Conservation Areas to investigate the possible impact of additional inflow under various backpumping cases. The modification of the model included Manning's roughness coefficient, depth of flow, width of hypothetical channels through marsh areas, rainfall input, seepage rate, etc. The use of the Monte Carlo technique for area computations was found to be easy and time saving both in area and weighting rainfall input to each node. Comparison of results generated by this modified model with the recorded values in Conservation Areas 1 and 2A indicated that the model not only can be a very good evaluation tool to simulate the hydraulic regime of the Conservation Areas system but also a proper tool for investigating the impact of additional inflow resulting from the backpumping related to the water use planning and management.     

试论安全生产中的几个关系

近年来 ,我国国民经济快速发展 ,但安全生产状况不容乐观 ,安全事故频繁发生 ,事故死亡人数居高不下。笔者在对 1993～ 2 0 0 0年我国安全生产数据进行分析的基础上 ,应用最小二乘法拟合技术研究安全生产与安全事故的内在联系 ;建立人均GDP、人均安全投入、人均安措投入、人均劳保投入与 10万人死亡率和亿元GDP死亡率的函数关系 ;探讨造成安全生产事故的原因 ;进一步完善了安全理论。上述对安全生产中若干问题研究的思路和方法 ,为安全生产的宏观决策和预防事故的发生提供了重要理论和科学依据。     

MaryPODS revisited: updated crash analysis and implications for screening program implementation

PROBLEM: Due to the relative scarcity of crashes, there has consistently been a problem with analyses that use crashes as a criterion measure in their analyses. METHOD: Previous analyses of the relationships between functional capacity measures and at-fault crash involvement for older drivers as reported in the NHTSA Model Driver Screening and Evaluation Program Final Technical Report have been updated to include one additional year of driving experience. Eighteen new at-fault crashes involving drivers who previously had no crash involvement were recorded for the Maryland Motor Vehicle Administration (MVA) test sample during this interval. The method of odds ratio (OR) calculation was used to examine the relationships between functional status predictors and the most salient among the safety outcome measures identified in the Maryland research. Peak valid OR values for the prior and current analyses were contrasted, and the stability of candidate pass-fail cut-points for each predictor relative to values identified in the Final Technical Report was examined. RESULTS: Results indicate that the predictive value of functional tests appears to decrease over time, particularly for the perceptual-cognitive measures. IMPACT ON INDUSTRY: The impact of these findings on programs and policies is to underscore a need for periodic reevaluation, spaced at the shortest practical intervals but not more than 2 years apart, in order for functional capacity screening to be applied effectively by licensing authorities, health care professionals, and others to reduce personal risk and enhance public safety.     

城市新增大气污染源合理布局判据的研究

试图提出了一种以环境损失作为评价污染源布局判据的观点，给出了大气环境损失系数的计算方法及污染源合理布局优化计算模型，并结合实例作了进一步的分析。     

机车司机行车安全监控系统应用研究

分析铁路行车安全监控系统的复杂性和现状;设计分局机车司机行车安全监控系统和数据流向;研制车载的机车司机工作状态实时监督系统;采用视频监控、图像处理技术以及人脸识别技术对机车司机工作状态进行识别并实时报警。经过实验证明:对司机长时间离开座位、睡眠和呆木状态有较高的识别率;同时对司机工作状态的图像信号进行压缩储存;管理人员可以回放检查。采用CDMA技术设计了地面对机车司机工作状态的视频监控子系统,可以把司机的工作状态图像及时传送到车站或调度中心。实际线路实验表明,该系统可以大大提高司机工作的安全性。