Microgeographic heterogeneity in spatial distribution and mtDNA variability of gray mouse lemurs (<Emphasis Type="Italic">Microcebus murinus</Emphasis>, Primates: Cheirogaleidae)

The objective of our study was to investigate the spatial distribution and genetic structure of a solitary primate at the microgeographical scale of adjacent local populations. We obtained spatial data and tissue samples for mtDNA analysis from 205 gray mouse lemurs (Microcebus murinus) captured along transects and within 3 grid systems within a 12.3 km² area in Kirindy Forest, western Madagascar. Our capture data revealed that, even though the forest was continuous, gray mouse lemurs were not evenly distributed, and that daily and maximum dispersal distances were significantly greater in males. The frequency distribution of 22 mtDNA D-loop haplotypes was highly skewed. Nine haplotypes were unique to males, indicating male-mediated gene flow from surrounding areas. The geographic distribution of haplotypes revealed that males were also more dispersed than females. Females with the same haplotype showed a tendency towards spatial aggregation, and the correlation between genetic and geographic distances was higher in females. In several areas of the forest, however, spatially clustered females were not of the same haplotype, and females were not always found in clusters. Hence, in contrast to suggestions from previous studies, matrilineal clustering is not the only way females are socially organized. In addition, our study revealed heterogeneity and patterns in population structure that were not evident at smaller spatial scales, some of which may be relevant for designing conservation strategies.Communicated by C. Nunn     

High genetic diversity of the symbiotic dinoflagellates in the coral Pocillopora meandrina from the South Pacific

Symbioses between dinoflagellates in the genus Symbiodinium (commonly referred to as zooxanthellae) and scleractinian corals are an essential feature for the maintenance of coral reefs. The fine-scale diversity and population structure of the zooxanthellae inhabiting the coral Pocillopora meandrina, a major reef building species in Polynesia, was examined. We used two polymorphic microsatellites to study seven populations from the South Pacific, whose host structuring has been previously investigated. The symbionts of P. meandrina showed high levels of diversity, with more than one zooxanthella genotype being identified in most of the host individuals. Genetic differentiation between symbiont populations was detected at a large scale (2,000 km) between the Tonga and the Society Archipelagos. Within the Society Archipelago, the two most remote populations (Tahiti and Bora-Bora; 200 km apart) were only weakly differentiated from each other. Statistical tests demonstrated that the symbiont genetic structure was not correlated with that of its host, suggesting that dispersal of the symbionts, whether they are transported within a host larva or free in the water, depends mainly on distance and water currents. In addition, the data suggests that hosts may acquire new symbionts after maternal transmission, possibly following a disturbance event. Lastly, the weak differentiation between symbiont populations of P. verrucosa and P. meandrina, both from Moorea, indicated that there was some host-symbiont fine-scale specificity detectable at the genetic resolution offered by microsatellites.     

Olfactory cues,genetic relatedness and female mate choice in the agile antechinus (<Emphasis Type="Italic">Antechinus agilis</Emphasis>)

Females show mate preferences for males that are genetically dissimilar to themselves in a variety of taxa, but how females choose these males is not clearly understood. In this study, we examined the effects of olfactory stimuli and genetic relatedness on female mate choice in a small carnivorous marsupial, the agile antechinus (Antechinus agilis), during two breeding seasons. Captive female antechinus in oestrus were provided with a combination of male urine and body scent from two novel males, one more genetically similar and one more dissimilar to the females, in a Y-maze olfactometer. Genetic relatedness between females and pairs of males was determined using highly polymorphic, species-specific, microsatellite markers. Females consistently chose to visit the scents of males that were genetically dissimilar to themselves first, spent significantly more time near the source of those scents and showed more sexual and non-exploratory behaviours near those scents. These data demonstrate that chemosensory cues are important in mate choice in the agile antechinus and that females prefer males that are genetically dissimilar to themselves.     

High degree of polyandry in Apis dorsata queens detected by DNA microsatellite variability

Workers of six colonies of the giant honeybee Apis dorsata from Sabah, Malaysia (five colonies) and Java (one colony) were genotyped using single locus DNA fingerprinting. The colonies from Sabah nested in colony aggregations of 5 and 28 nests respectively on two trees. Three DNA microsatellite loci (A14, A76, A88) with a total of 27 alleles provided sufficient genetic variability to classify the workers into distinct sub-families revealing the degree of polyandry of the queens. Queens mated on average with 30.17 ± 5.98 drones with a range from 19 to 53. The average effective number of matings per queen was 25.56 ± 11.63. In the total sample of 192 workers, 22 individuals were found that were not offspring of the colony's queen. Three of these were potentially drifted offspring workers from genotyped queens of colonies nesting on the same tree.     

Use of genetic algorithms to select input variables in decision tree models for the prediction of benthic macroinvertebrates

Predicting freshwater organisms based on machine learning is becoming more and more reliable due to the availability of appropriate datasets, advanced modelling techniques and the continuously increasing capacity of computers. A database consisting of measurements collected at 360 sampling sites in non-navigable watercourses in Flanders was applied to predict the absence/presence of benthic macroinvertebrate taxa by means of decision trees. The measured variables were a combination of physical–chemical (temperature, pH, dissolved oxygen concentration, conductivity, total organic carbon, Kjeldahl nitrogen and total phosphorus), structural (granulometric analysis of the sediment, width, depth and flow velocity of the river) and two ecotoxicological variables. The predictive power of decision trees was assessed on the basis of the number of Correctly Classified Instances (CCI). A genetic algorithm was introduced to compare the predictive power of different sets of input variables for the decision trees. The number of input variables was reduced from 15 to 2–8 variables without affecting the predictive power of the decision trees significantly. Furthermore, reducing the number of input variables allowed to ease the identification of general data trends.     

云南粳稻耐低磷特性的主基因加多基因遗传分析

磷高效水稻培育是提高土壤潜在磷利用效率的一种途径,通过对云南粳稻耐低磷特性的主基因加多基因遗传分析,可以明确耐低磷特性主基因的存在、对数及遗传效应的大小,为磷高效育种提供理论依据。本试验通过云南主栽粳稻品种合系35(Oryza sativa)与耐低磷极强早旱谷(Oryza sativa)配制P1、P2、F1、F2或F3世代,在云南省农科院进行低磷胁迫试验,对耐低磷鉴定和主基因加多基因联合遗传分析,结果表明:早旱谷的分蘖和株穗产量基因受两对主效基因 多基因相互配合遗传控制的,两对主基因间存在上位性效应,两主基因间效应差异较大。分蘖力F2主基因遗传率为58.82%~72.13%,F3主基因遗传率为45.88%~57.96%;株穗产量F2主基因遗传率60.94%~83.08%,F3主基因遗传率为62.20%~75.80%。因此育种中应当充分重视主基因的利用,利用主基因应以第一对主基因的加性效应为主,对后代耐低磷性状的选择宜从F2代开始,杂交的后期世代也要注意耐性的选择。     

Optimisation algorithms for spatially constrained forest planning

We compared genetic algorithms, simulated annealing and hill climbing algorithms on spatially constrained, integrated forest planning problems. There has been growing interest in algorithms that mimic natural processes, such as genetic algorithms and simulated annealing. These algorithms use random moves to generate new solutions, and employ a probabilistic acceptance/rejection criterion that allows inferior moves within the search space. Algorithms for a genetic algorithm, simulated annealing, and random hill climbing are formulated and tested on a same-sample forest-planning problem where the adjacency rule is strictly enforced. Each method was randomly started 20 times and allowed to run for 10,000 iterations. All three algorithms identified good solutions (within 3% of the highest found), however, simulated annealing consistently produced superior solutions. Simulated annealing and random hill climbing were approximately 10 times faster than the genetic algorithm because only one solution needs to be modified at each iteration. Performance of simulated annealing was essentially independent of the starting point, giving it an important advantage over random hill climbing. The genetic algorithm was not well suited to the strict adjacency problem because considerable computation time was necessary to repair the damage caused during crossover.     

Seasonal polydomy and unicoloniality in a polygynous population of the red wood ant Formica truncorum

Ant colonies may have a single or several reproductive queens (monogyny and polygyny, respectively). In polygynous colonies, colony reproduction may occur by budding, forming multinest, polydomous colonies. In most cases, budding leads to strong genetic structuring within populations, and positive relatedness among nestmates. However, in a few cases, polydomous populations may be unicolonial, with no structuring and intra-nest relatedness approaching zero. We investigated the spatial organisation and genetic structure of a polygynous, polydomous population of Formica truncorum in Finland. F. truncorum shifts nest sites between hibernation and the reproductive season, which raises the following question: are colonies maintained as genetic entities throughout the seasons, or is the population unicolonial throughout the season? Using nest-specific marking and five microsatellite loci, we found a high degree of mixing between individuals of the population, and no evidence for a biologically significant genetic structuring. The nestmate relatedness was also indistinguishable from zero. Taken together, the results show that the population is unicolonial. In addition, we found that the population has undergone a recent bottleneck, suggesting that the entire population may have been founded by a very limited number of females. The precise causes for unicoloniality in this species remain open, but we discuss the potential influence of intra-specific competition, disintegration of recognition cues and the particular hibernation habits of this species.     

Effects of intracolony variability in behavioral development on plasticity of division of labor in honey bee colonies

There is a genetic component to plasticity in age polyethism in honey bee colonies, such that workers of some genotypes become precocious foragers more readily than do workers of other genotypes, in colonies lacking older bees. Using colonies composed of workers from two identifiable genotype groups, we determined that intracolony differences in the likelihood of becoming a precocious forager are a consequence of differences in rates of behavioral development that are also evident under conditions leading to normal development. An alternative hypothesis, that differences in the likelihood of becoming a precocious forager are due to differences in general sensitivity to altered colony conditions, was not supported. In three out of three trials, workers from the genotype group that was more likely to exhibit precocious foraging in single cohort colonies also foraged at relatively younger ages in colonies in which workers exhibited normal behavioral development. In contrast, in three out of three trials, workers from the genotype group that was more likely to exhibit precocious foraging in single-cohort colonies did not show disproportionately more overaged nursing in colonies in which workers exhibited delayed development. These results indicate that genotypic differences in plasticity in age-related division of labor are based on genotypic differences in rates of behavioral development.     

湖泊营养状态评价的普适指数公式及效果检验

适当设定湖泊富营养化指标“参照”值 ,将修正的Carlson指数公式中的指标值用相对于“参照”值的指标“相对值”替换 ,公式中的参数可视为与指标特性无关。对公式中的待定参数采用遗传算法进行优化 ,得到对多项指标都通用的营养状态普适指数公式。用折衷激励功效函数法对单项指标的TSIG 指数赋权 ,计算综合指数。公式的正确性已被用于我国数十个湖泊、水库的营养状态评价检验得到证实。该公式计算简单、实用 ,具有客观性、可比性和通用性