基于多目标分布估计算法的地铁网络化应急站点选址

为提升地铁网络的应急救援能力,提出兼顾地下和地面交通两种运输方式的应急资源网络化共享模式,并建立多目标优化模型,对应急站点进行优化选址。模型以应急站点数量最少和总响应时间最短为优化目标,地铁车站重要程度为关键因素,求解各地铁车站的优化覆盖方案。提出了一种多目标分布估计算法,该算法采用拥挤比较算子和锦标赛选择算法获取优势群体,借助概率模型描述可行解的离散分布,并不断更新概率模型来实现种群的进化。运用建立的模型和算法求解北京市局部地铁网络应急站点选址问题,最终获得满足地面交通运输时效、并符合问题优化目标的Pareto最优解集。与NSGE-Ⅱ算法相比,多目标分布估计算法提前123代开始收敛,且针对最小化问题,多目标分布估计算法最优解的两个目标函数值较NSGE-Ⅱ算法分别减小64%和29%。可见,模型符合地铁应急站点选址问题的决策规则;多目标分布估计算法有效地缩小了决策空间,它比NSGE-Ⅱ算法更快地收敛,且能够获得更为优化的应急站点选址方案。     

一种新的结构可靠度通用计算方法

根据可靠度指标β的几何定义,运用具有全局优化能力的改进遗传算法,提出一种新的结构可靠度通用计算方法。针对可靠度几何优化模型搜索区域大而可行区域小的特点,在可靠区域和失效区域分别产生初始种群,在两种群之间通过不断迭代的二分法,使二分点不断接近极限状态曲面(线),从而产生可行解。对可行解进行选择、交叉、变异,最后求出全局最优解。该方法不需要使用结构功能函数的偏导数,对于功能函数不能明确表达的可靠度问题尤为适用;对于功能函数可以明确表达但求导复杂的问题,可省去求导过程;可任意设定初始值,且不受结构功能函数凹凸性的限制;通过算例验证,所提方法具有较高的精度和效率。     

土著厌氧菌修复有机氯污染土壤的方法探索

针对工业场地有机氯污染土壤探索微生物修复的方法,试验表明:添加土著厌氧菌菌液及营养液的试验组,污染物去除率比空白组明显提高。基因测定结果显示,试验土壤中至少存在具有降解有机氯污染物能力的3种菌种。     

Assessment of susceptibility of olive cultivars to the Bactrocera oleae (Gmelin, 1790) and Camarosporium dalmaticum (Thüm.) Zachos & Tzav.-Klon. attacks in Calabria (Southern Italy)

Within the framework of research concerning the application of techniques alternative to chemical pesticides for control of parasites, the C.R.A. Experimental Institute for Olive Growing for many years has been performing a large investigation in order to detect sources of genetic resistance in olive germplasm. In the present study we observed the behavior related to the olive fly (Bactrocera oleae) infestation and Camarosporium dalmaticum infection of ten olive cultivars farmed under the same agronomic and climatic conditions in Calabria, Southern Italy. The sampling and the data collecting were carried out in three different ripening times.The drupe amount of oleuropein and cyanidine was detected by laboratory analyses in order to verify a possible correlation between these molecules and the level of infestation/infection of the above-mentioned parasites. The obtained data were submitted to analysis of variance. In relation to the fungal infection the results displayed that cvs Tonda nera dolce showed the lowest susceptibility, while the cv Giarraffa turned out to be the most susceptible. The less susceptible cultivars to the phytophagous were Tonda nera dolce and Bhardi Tirana. Since the less susceptible cultivar to olive fly attacks are the same observed in relation to the susceptibility to olive fruit rot, it is suggested a relation between the olive fly infestation and the fungal infection. It suggests the utility to achieve these results both to transfer directly to the farmers' world and to emphasize ecosystem health and biodiversity conservation.     

The Future of Baltic Sea Populations: Local Extinction or Evolutionary Rescue?

Environmental change challenges local and global survival of populations and species. In a species-poor environment like the Baltic Sea this is particularly critical as major ecosystem functions may be upheld by single species. A complex interplay between demographic and genetic characteristics of species and populations determines risks of local extinction, chances of re-establishment of lost populations, and tolerance to environmental changes by evolution of new adaptations. Recent studies show that Baltic populations of dominant marine species are locally adapted, have lost genetic variation and are relatively isolated. In addition, some have evolved unusually high degrees of clonality and others are representatives of endemic (unique) evolutionary lineages. We here suggest that a consequence of local adaptation, isolation and genetic endemism is an increased risk of failure in restoring extinct Baltic populations. Additionally, restricted availability of genetic variation owing to lost variation and isolation may negatively impact the potential for evolutionary rescue following environmental change.     

Elevated ozone affects the genetic composition of Plantago lanceolata L. populations

The genetic composition and diversity of Plantago lanceolata L. populations were analysed using amplified fragment length polymorphism (AFLP) as well as simple sequence repeat (SSR) markers to test for differences in an old semi-natural grassland after five years of treatment with ambient or elevated ozone (O3) using a free-air fumigation system. Genetic diversity in populations exposed to elevated O3 was slightly higher than in populations sampled from control plots. This effect was significant for AFLP-based measures of diversity and for SSR markers based on observed heterozygosity. Also, a small but significant difference in genetic composition between O3 treatments was detected by analysis of molecular variance and redundancy analysis. The results show that micro-evolutionary processes could take place in response to long-term elevated O3 exposure in highly diverse populations of outbreeding plant species.     

Multi-generation studies with Chironomus riparius--effects of low tributyltin concentrations on life history parameters and genetic diversity

While toxicological data are available for numerous chemicals from standard tests, little is known on effects of pollutants over several generations or regarding chronic effects of chemicals on genetic diversity. Within the experiments, effects of the model pollutant tributyltin (TBT) were investigated over eleven generations at a sublethal TBT concentration of 4.46 μg as Sn kg⁻¹ sediment dw on life-cycle parameters and genetic variability of Chironomus riparius. Moreover, the adaptation potential towards TBT was determined. This experimental design enables the identification of TBT effects on life-cycle parameters and the determination of a potential extinction risk caused by chronic exposure. Furthermore, effects on the genetic structure can be determined, which are not predictable based solely on knowledge of the toxic mode of action of the chemical.

Genetic variety was determined via microsatellite analysis, measuring individual length differences of highly variable satellite DNA fragments. For the identification of changes in tolerances towards the stressor, acute and chronic toxicity experiments were conducted.

During the multi-generation study, significant effects on development and reproduction were determined. For some generations, the emergence was significantly (p < 0.05) delayed under TBT exposure. Reproduction seems to be a sensitive parameter as well, whereby females laid significantly larger egg masses (p < 0.05) in the latter generations. TBT did not affect the population growth rate nor the genetic variability, while clear deviations from the Hardy–Weinberg equilibrium appeared. The study also provides strong evidence for the acquirement of a higher tolerance towards the stressor in the TBT-exposed group.     

A GIS-based approach for assessing the regional conservation status of genetic diversity: an example from the southern Appalachians

Conserving genetic diversity requires an assessment of the distribution of genetic variants in relation to patterns of land use and environmental variation at a regional scale. This assessment requires a novel approach to integrating and analyzing the genetic and environmental data across spatial scales. To explore the integration of genetic data with other geospatial data sets, we developed a GIS-based approach for examining patterns of genetic diversity for several species of salamanders in southern Appalachians. The genetic data, from allozyme surveys in the genetics literature, were integrated into a GIS database along with related attributes including population identifications and spatial locations. Using existing geospatial data, we classified sample locations as being either protected from anthropogenic disturbance (e.g., National Parks, Wilderness Areas) or as unprotected (e.g., private lands, multiple-use lands in National Forests). We used multidimensional scaling of allelic frequencies and contributions of populations to interpopulation differences in allelic richness to determine which populations had genetic characteristics most different from other populations in the sample. Measures of genetic differentiation were integrated into the GIS database to facilitate spatial analysis and visualization of the indices in relation to land use. This approach was useful for both identification of populations with components of genetic variation that were not well represented at protected sites and for identifying areas of species distributions where more genetic sampling would be necessary to make informed management decisions. Our approach could be readily adapted for use by managers and geneticists working with other species and types of genetic markers.     

水环境质量评价的普适指数公式

在适当设定水质指标的“基准”值情况下,用相对于“基准”值的指标相对值替换对数形式的指数公式中的水质指标值,采用遗传算法优化不同指标的指数公式中的参数,得到对多项指标通用的水质普适指数公式;并用折衷型激励功效函数将单项指标的指数加权为综合指数。公式的正确性已被多处水质评价检验。