TTC－脱氢酶活性检测方法的研究

以好氧消化污泥为材料，对影响脱氢酶活性测定的样品前处理、生化培养、酶反应终止剂、三苯基甲Ｚａ（ＴＦ）苯取剂以及萃取条件等问题进行了系统地研究，进而提出了检测脱氢酶活性的常温萃取测定法。这种方法已在剩余污泥好氧消化处理、生物转盘处理生物制品废水以及陶粒柱反应器低温生物预处理饮用水等研究中应用，效果良好。     

假单胞菌(Pseudomonas sp.)表面活性物质产生与性能

从广州猎德污水处理厂的二沉池污泥中分离到1株产表面活性剂的细菌,经镜检及一系列生理生化试验,鉴定为假单胞菌属(Pseudomonas sp.)。试验结果表明,菌株接种培养96h后,培养液可以使汽油层的乳化率达到100%;产表面活性物质的最佳的碳源和氮源分别为葡萄糖和NH4NO3,最大的排油直径均超过100mm;在偏碱性(pH 8￣9)的培养条件下生长良好,培养液的排油活性明显优于中性及酸性条件;盐离子浓度较高时能够生长并且大量产表面活性物质;通气量对培养液的表面活性有较大影响,摇瓶培养比通气培养更有利于保持培养液的表面活性。     

SiO_2-TiO_2纳米管的制备及其光催化性能的研究

文章采用水热法制备SiO2-TiO2纳米管,通过扫描电镜(SEM)和透射电镜(TEM)考察了该纳米管的结构形态,并用X-射线衍射考察其晶体结构。实验结果表明所制得的纳米管是锐钛晶型,其两端开口,具有多层管壁;管径为8nm左右,管壁厚1～2nm。光催化降解酸性橙Ⅱ实验表明:SiO2-TiO2纳米管光催化速率比TiO2纳米管高出10%。这可能是因为Si元素的存在增大了钛纳米管的比表面积,同时SiO2的存在能降低光生电子和空穴的复合率。     

关于环境监理稽查

环境监理稽查包括两方面内容：一是上级环境保护行政主管部门跨越行政级别直接查处环境行政违法案件；二是查处违法环境行政行为，对其作用进行了探讨。     

Study on nitrile-degrading microorganisms

Two strains of bacteria were isolated from nitrile polluted soils, and identified as Corynebacterium boffmanii and Arthrobacter flavescens. Acetonitrile, propionitrile, butyronitrile and acrylonitrile were degraded by these bacteria to yield corresponding amides, carboxylic acids and ammonia. The nitrile-degrading abilities of these strains were investigated. The removal rates for the nitrile were nearly 100%, after these bacteria were grown in medium containing 10000 ppm of aceto-, propio-, or butyronitrile at 28 ℃ for 24h. When the reaction mkture consisting of 5000 ppm of above mentioned nitriles or acrylonitrile and 20g (dry cell) /L resting cells of the two strains in 0.06mol/L phosphate buffer (pH7.5) was incubated separately at 25 ℃ with moderate shaking for 15 or 45 min, the nitrile could be degraded completely. The optimum growth conditions for C.hoffmanii and A.flavescens were studied as well.     

铜离子冲击下活性污泥微生物多样性的分子生态学分析

采用基于 16SrDNA序列分析方法 ,在长度多态 (LPM)和 16SrDNA的GC含量二维水平分析了受重金属污染的活性污泥系统内细菌的优势种类和多样性 .设计的PCR引物可以将细菌分为三大类 :即 1)proteobacterialα&δ(变形杆菌α&δ) ,2 )pro teobacterialβ&γ(变形杆菌 β&γ) ,以及 3)flexibacter(屈桡杆菌 )和革兰氏阳性菌 .分析了未受重金属驯化和受重金属驯化的两类活性污泥系统在重金属作用下优势种和多样性的变化 ,结果显示未驯化的活性污泥系统多样性减少但优势种的变化微小 ,而驯化系统优势种有较大的变化 ,但多样性基本不变 .这一结果证实驯化有助于提高微生物对重金属的抗性     

Sanfilippo D syndrome: Correction of glucosamine-6-sulphatase deficiency following fibroblast culture in chang's media

The de-O-sulphation of α-linked glucosamine-6-sulphate residues in heparan sulphate requires a specific sulphatase, glucosamine-6-sulphatase, which has been shown to be deficient in tissues of Sanfilippo D, or mucopolysaccharidosis type IIID (MPS IIID), patients. MPS IIID fibroblasts cultured in Basal Eagle's medium supplemented with either fetal calf serum or heat-inactivated fetal calf serum, MDCB or Ultraserg media had residual glucosarnine-6-sulphatase activities towards a heparin-derived trisaccharide substrate, O-(α-N-acetylglucosamine-6-sulphate)-(1→4)-L -O-(α-iduronic acid-2-sulphate)-(1→4)-D -O-2,5-anhydro [1-³H]mannitol-6-sulphate, GlcNAc6S-IdoA2S-anM6S, which were less than 1 per cent of the normal range for fibroblasts cultured in Basal Eagle's medium supplemented with fetal calf serum. However, the glucosamine-6-sulphatase activities of MPS IIID fibroblasts grown in Chang's medium were similar to the activities in normal control fibroblasts which were cultured in Basal Eagle's medium. These results indicate that caution is required for prenatal diagnosis of MPS IIID patients using chorionic villi or amniotic cells cultured in Chang's medium.     

甲氰菊酯在土壤中的降解与移动性

采用模拟试验和田间试验相结合的方法,研究了甲氰菊酯在南方菜地土壤中的降解和移动.得出了一些基本数据,为甲氰菊酯在菜地(旱地)生态中的安全使用,提出了合理的评价.     

BOD微生物传感器的研究

本实验从活性污泥中分离筛选出一株对广泛底物具有外源呼吸的假单胞菌(Pseudomonas sp.A_4)。用该种菌株制成的BOD微生物传感器可在15min内完成一个样品BOD的测定。该法与传统方法有较好的相关性,可连续使用六周以上。     

旅游踩踏对土壤微生物生物量碳、氮、磷的影响

为了探明旅游活动对张家界国家森林公园土壤微生物活性及多样性的影响,保护土壤生态平衡及合理开发自然保护区提供理论依据,进行了旅游踩踏对土壤微生物生物量碳、氮、磷的影响研究.结果表明,在所设的3个试验区中,0～5cm土壤层的微生物生物量碳、磷受到旅游踩踏的影响最严重,5～15cm土壤层的微生物生物量碳、磷受到旅游踩踏的影响较严重,而15～25cm土壤层的微生物生物量碳、磷受到旅游踩踏的影响最轻,旅游踩踏对所设3个试验区中3个层次土壤的微生物生物量碳、磷的影响均达到了显著水平(p＜0.05).从旅游踩踏对3个土壤层的微生物生物量氮的影响程度来看,0～5cm土壤层的微生物生物量氮受到的影响最严重,5～15cm土壤层的微生物生物量氮受到的影响较严重,而15～25cm土壤层的微生物生物量氮受到的影响最轻,背景区与缓冲区15～25cm土壤层的微生物生物量氮差异没有达到显著水平.说明张家界国家森林公园土壤微生物遭到了旅游踩踏的破坏,抵御外界干扰的能力已受到了旅游踩踏的破坏.