Purification and characterization of extracellular poly(3-hydroxybutyrate) depolymerases

Five extracellular PHB depolymerases of bacteria isolated from various sources were purified to electrophoretic homogeneity and compared with known extracellular PHB depolymerase fromAlcaligenes faecalis T1. The molecular mass of these enzymes were all around 40–50 kDa. Nonionic detergent, diisopropylfluorophosphate and dithiothreitol inhibited the PHB depolymerase activity of all these enzymes. Trypsin abolished PHB depolymerase activity, but not theD-3-hydroxybutyric acid dimer hydrolase activity of all the enzymes. These results showed that the basic properties of these PHB depolymerases resemble those of theA. faecalis T1 enzyme. Analysis ofN-terminal amino acid sequence of the purified enzymes revealed that these enzymes includingA. faecalis T1 enzyme fall into three groups.     

关于职工情境意识的研究综述

对情境意识的定义、活动机制、测量方法及强化方法等进行了述评。分析了关于情境意识研究的问题及趋势。     

五氯酚对环境污染及居民健康影响研究

中国长期以来使用五氯酚纳盐杀灭亡血吸虫的中间宿主──—钉螺．为评价它对环境的污染及居民健康危害，１９９０－１９９３年在我省开展了五氯酚环境污染及对居民健康影响研究．在研究区采集环境样品（空气、水体、土壤及底泥、蔬菜、肉、蛋、鱼等）共１７３份；生物材料（人血、尿）共４５７份，分析五氯酚浓度．结果在明，在五氯酚使用区，环境中ＰＣＰ浓度稍高于对照区，而生物材料尿、血中ＰＣＰ浓度明显高于对照区．全血胆碱脂酶活性用药区明显低于对照区，统计学分析有显著差异（Ｐ＜０．００１），证实了长期低剂量使用可造成环境及生物蓄积．     

AAS与ICP－AES间接测定天然水中硫酸盐

本文以与过量形成ＢａＳＯ＿４沉淀反应为基础，较详细地讨论了反应条件、从而拟定了用ＡＡＳ和ＩＣＰ－ＡＥＳ间接测定天然水中的方法、确定了方法的精密度，ＡＡＳ法的ＲＳＤ％为１．５，ＩＣＰ－ＡＥＳ法为３．２．两种方法对比分析的结果接近，误差为１．０％（相对）。用于天然水中的测定，获得满意的结果。     

基于GIS的汕头市城市综合防灾系统的研究方案及实施框架

在研究汕头市地震、风、洪潮、火等多种灾害的危险性、成灾模式和损失估计的基础上,应用地理信息系统(GIS)技术建立可视化的计算机综合防灾系统,可以提高城市的防灾能力。本文针对基于GIS的城市综合防灾系统的建立,提出了较为具体的研究方案和实施框架,便于课题组各项研究工作的开展和统一。     

FACTORS CONTROLLING RUNOFF NUTRIENT LOSS FROM CULTIVATED LAND IN EASTERN SOUTH DAKOTA1

ABSTRACT A stepwise multiple regression was used to evaluate the relative importance of antecedent-, event-, and high-intensity-precipitation amounts; runoff volume; and precipitation nutrient contents and concentrations in determining nutrient losses in runoff. Plots were fertilized at high, medium, and low rates and had cultivation ridges oriented up-and-down slope or on the contour. Runoff volume was the important factor in determining the loss of NO₃, soluble PO₄, total PO₄, and soluble K ions in runoff from fallow, corn, or soybean plots. NH₄-N loss was controlled mainly by the precipitation content and antecedet precipitation since fertilizer had been applied. Nutrient losses were greater from plots fertilized at the high rate and from plots with cultivation ridges oriented up-and-down slope rather than on the contour.     

A study of compliance with environmental regulations of ISO 14001 certified companies in Korea

This article investigates the impact of ISO 14001 certification on the compliance with environmental regulations by Korean companies. The impact of ISO 14001 certification on the industry was studied through a questionnaire survey and the compliance of environmental regulations were investigated using government-released data. The motivation for an environment management system was a result of the current international situation and the need to maintain fair competition. ISO 14001 certification has been recognized as an essential strategy for industrial competition and to improve company/product recognition. The certified and non-certified companies' environmental regulation violation (ERV) rates were 3.5% and 11.6%, respectively, in 1997. In 1998, the ERV rate had an eight-time difference with 1.0% and 8.5% for certified and non-certified companies, respectively. Annual regulation violation rates were reduced from 3.5% in 1997 to 1.0% in 1998 with certified companies and from 11.6% in 1997 to 8.5% in 1998 with their non-certified counterparts, respectively. ISO 14001 certified companies showed more improvement than non-certified companies in regards to environmental performance.     

Refuse treatment options: a case study

Records in public archives were evaluated for the period 1981-1991. Simple surveys of peoples and of technology and physical premises provided insight to gauge the attitudes of a local community in the Melbourne municipality and the Melbourne City Council towards composting of organic waste materials. There is a lag in perception and attitudes between the local community and the local government towards composting of organic refuse as a solid waste treatment option. The simple methodology of the study still made it possible to verify past and present perceptions and attitudes.     

ARCGIS‐SWAT: A GEODATA MODEL AND GIS INTERFACE FOR SWAT1

This paper presents ArcGIS‐SWAT, a geodata model and geographic information system (GIS) interface for the Soil and Water Assessment Tool (SWAT). The ArcGIS‐SWAT data model is a system of geodatabases that store SWAT geographic, numeric, and text input data and results in an organized fashion. Thus, it is proposed that a single and comprehensive geodatabase be used as the repository of a SWAT simulation. The ArcGIS‐SWAT interface uses programming objects that conform to the Component Object Model (COM) design standard, which facilitate the use of functionality of other Windows‐based applications within ArcGIS‐SWAT. In particular, the use of MS Excel and MATLAB functionality for data analysis and visualization of results is demonstrated. Likewise, it is proposed to conduct hydrologic model integration through the sharing of information with a not‐model‐specific hub data model where information common to different models can be stored and from which it can be retrieved. As an example, it is demonstrated how the Hydrologic Modeling System (HMS) ‐ a computer application for flood analysis ‐ can use information originally developed by ArcGIS‐SWAT for SWAT. The application of ArcGIS‐SWAT to the Seco Creek watershed in Texas is presented.