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51.
52.
Dr Marianne Johansen Marian Knight Edward J. Maher Kim Smith Ian L. Sargent 《黑龙江环境通报》1995,15(10):921-931
Trophoblast deportation is known to occur in normal human pregnancy, but it is not yet clear whether these cells routinely enter the maternal peripheral circulation and are available as a source of fetal DNA for non-invasive prenatal diagnosis of genetic disorders. To resolve this issue requires an efficient method of enriching trophoblast from maternal blood combined with a means to confirm its identity. Five different techniques were tested on ten retroplacental blood samples to determine the most sensitive and operator-efficient method. Lysis of red cells alone gave the best recovery of trophoblast but had to be discounted, together with Ficoll density gradient centrifugation, due to the very low purity and the excessive time required. Fluorescence-activated cell sorting (FACS) of pre-enriched trophoblast resulted in the lowest recovery rate (8 per cent) despite a 3250-fold enrichment and a very high purity. Immunomagnetic beads (Dynabeads) coated with anti-CD 16 antibody proved to be the best method for the subsequent immunocytochemical characterization of deported trophoblast. However, IO beads coated with anti-CD45 antibody may be more useful for isolating trophoblast for prenatal diagnosis due to the high purity, enrichment (32-fold), and recovery rate (78 per cent) obtained with this method. 相似文献
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The karyotype of cultured amniotic fluid cells obtained on the indication of advanced maternal age was shown to be a mosaic 45,X/46,X,r(?). The small size and banding pattern made it difficult to determine whether the ring was derived from and X or a Y chromosome, or even from an autosome. By using an X-centromeric probe and fluorescence in situ hybridization (FISH), we demonstrated the ring to have an X centromere. Thus, a more complete genetic counselling was possible. This confirms the usefulness of FISH in identifying and characterizing this and other chromosome rearrangements in prenatal diagnosis. 相似文献
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V. Sivakumar V. Ravi Verma P.G. Rao G. Swaminathan 《Journal of Cleaner Production》2007,15(18):1813-1818
Focus on eco-friendly processing techniques makes vegetable tanning a viable option in leather processing and establishes the subsequent need for the more efficient methods of extraction in tannin manufacture. Application of ultrasound has been tried in the extraction of tannins from myrobalan nuts in order to improve the extraction efficiency, to perform the extraction under milder process conditions and to reduce the process time. The influence of process parameters such as ultrasonic output power, time and temperature has been studied. Scale-up trials and the use of ultrasound in pulse mode have also been attempted. The results show that a three- to fivefold improvement is possible with ultrasonic output from 20 to 100 W. Extraction efficiency has been calculated from the maximum extractable materials from myrobalan nuts. Extraction efficiency is found to be 90% for ultrasound, 100 W without external heating as compared to 77% for control process at 70 °C for 4 h. Therefore, ultrasound could be employed even dispensing with provision for temperature controls. The use of ultrasound in pulse mode offered 70% extraction efficiency of continuous mode. Scale-up trials indicate that there exists an optimum ultrasonic output power depending on the amount of nuts used, to achieve better extraction efficiency. The effectiveness of ultrasonically extracted tannin solution has also been tested in the tanning process for its applicability. The degree of tanning efficacy has been assessed by shrinkage temperature measurement. The results indicate that ultrasonically extracted tannin solution is suitable for tanning process. Therefore, application of ultrasound in tannin extract manufacture is a viable option with added advantages. 相似文献
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浙东南下白垩统火山岩极为发育,岩石组合与浙江中部永康群差异甚大,称之为馆头组和朝川组似不合适。文成县峃口镇龙潭山(又称龙井山)剖面具代表性,拟以此部面为基础建立梅坪组和龙潭山组,合称文成群。与永康群对比,梅坪组大体相当馆头组,龙潭山组与朝川组和方岩组对比。 相似文献
59.
赵子维 《防灾技术高等专科学校学报》2005,7(4):119-121
本文分析了求知欲的由来及消退原因,“厌学症”的形成及治疗方案,试图唤起学生的求知欲,治疗他们的“厌学症”。 相似文献
60.
Liu Zhenyu Yang Fenglin Quan Xie Zhang Xiaohong 《Frontiers of Environmental Science & Engineering in China》2007,1(2):166-171
A QWASI model dependent on temperature is parameterized to describe the long-term fate of persistent organic pollutants (POPs)
in the Liao River. The model parameters, namely fugacity capacity, degradation rate, and transfer coefficient, are profoundly
affected by temperature. This model is used to simulate the fate of γ-hexachlorocyclohexane (γ-HCH) in the lower reaches of the Liao River from 1998 to 2008. Modeling results show that γ-HCH fugacity capacities in air, water, and sediment increase as temperature decreases, and the transfer and transformation
rate coefficients increase as temperature increases. The variations of transfer and transformation parameter D values depend on fugacity capacities, and transfer and transformation coefficients simultaneously. The performance of the
model is evaluated by comparing the predicted and observed concentrations in the water and sediment of the Liao River. The
predicted values agree well with the observed value in the order of magnitude, in most cases within the factor of 3. It is
believed that the model is appropriate for simulating the long term fate of POPs in the Liao River.
Translated from Environmental Science, 2006, 27(1): 121–125 [译自: 环境科学] 相似文献