辽东湾大凌河口湿地土壤微生物群落分析

土壤微生物对湿地生态系统的物质转化和能量流动具有重要作用,目前对天然湿地的微生物资源及群落结构缺少研究。应用PCR-DGGE技术研究辽东湾大凌河口湿地土壤中的微生物群落结构,并对其群落组成进行分析,结合冗余分析（RDA）方法考察环境因子对微生物群落结构的影响。共设定7个站位采集土壤样品,直接提取样品中微生物的总DNA,以巢式PCR扩增细菌16S rDNA-V3区片段,应用变性梯度凝胶电泳（DGGE）技术分离片段,研究河口区湿地土壤细菌的群落结构。结果表明：辽东湾大凌河口区土壤具有较为丰富的细菌种群,主要包含Proteobacteria、Actinobacteria和Bacteroidetes几大类门,且同一季节采集的样品在聚类分析图上聚类成簇。RDA分析表明,土壤的温度与盐度2个变量分别解释了6.5%、2.3%的土壤细菌群落结构变化,表明大凌河口区土壤的群落结构变化是多种环境因子共同作用的结果,其中温度的影响略大于盐度。     

Characteristics of pollutants behavior in a stormwater constructed wetland during dry days

A stormwater wetland treating non-point source pollution (NPS) from a 64 ha agricultural watershed was monitored over a period of five months. The results indicated that pH and dissolved oxygen (DO) were increased in the wetland due to the algal growth. The highest total suspended solids (TSS) concentration was observed in the aeration pond due to the resuspension of solids, decreased in the wetland. The respective decreases in total nitrogen (TN) and total kjeldahl nitrogen (TKN) were 15.9% and 28.7% on passing through the wetland. The nitrate and ammonia were increased by 45.4% and decreased by 79.9%, respectively. These variations provided strong evidence for the existence of nitrification. The total phosphorus (TP) and phosphate had respective reductions of 52.3% and 58.2% over the wetland. The total chemical oxygen demand (TCOD) and soluble chemical oxygen demand (SCOD) were also decreased. Generally, the TN, TP and phosphate removal efficiencies were positive. These positive removal efficiencies were mainly due to microbial activities, uptake by plants, and chemical precipitation at high pH. Negative removal efficiencies can be caused by continuous rainfall activities, with short antecedent dry days (ADDs) and unstable hydraulic conditions, some other biogeochemical transformations and algal growth also being important parameters.     

七里海湿地土地利用变化及其景观格局演替分析

通过遥感手段,利用大量的ETM、TM、MSS遥感影像,从土地覆盖变化、景观格局指数等角度,分析了近30年七里海湿地的土地利用变化特点及景观演替趋势特征。详细分析了七里海内水域、芦苇等用地类型的转化趋势与成因,并通过多年来景观指数对比的方法揭示了该区域景观连通性和破碎化程度的变化趋势。研究结果表明：由于入境水量减少,1976到2009年期间七里海湿地水域面积减少了80％,之后由于保护区的建立,水域面积持续增长。期间以保护区建立为节点,主要的土地利用类型变化趋势是水域-芦苇-水域。1976到2009年期问七里海总体聚集度指数从95．75下降到87．54、水面的破碎化指数从6．42上升到10．74,反映出区域景观连通性变差,破碎化程度在加剧,人类活动对环境影响越来越深刻。     

沿海湿地生态系统健康预警指标体系的设计

依据压力-状态-响应(presure-state-response,PSR)模型,分析了沿海湿地生态系统健康的相关影响因素,通过问卷调研和专家访谈法对影响因素进行调适,并对其进行信度检测,构建了湿地生态系统健康预警指标体系,采用层次分析法确定权重;进一步将湿地生态系统健康指标体系划分为5个健康等级,以江苏省为例,给出各项指标相应的健康等级标准,并设计了反映湿地生态系统健康状况的预警灯显表,可为湿地管理和决策部门制定政策提供依据。     

人工增氧型复合湿地污染物净化效果

针对传统人工湿地负荷低、硝化能力弱和易堵塞的缺点,结合曝气生物滤池与人工湿地强化理论的研究成果,自主开发了人工增氧型复合湿地工艺,即生物接触氧化预处理+微曝气垂直流湿地+水平潜流湿地。通过对滇池北岸城郊混合制污水处理的模型试验,分析该工艺流程各净化单元的污染物去除效果。结果显示,人工增氧型复合湿地对不同水力负荷和污染负荷都体现了较强的缓冲调节能力和较高的净化效果。对污染物的总去除率分别为SS 94.5%、CODCr71.3%、氨氮72.3%,在进水总氮10.0 mg.L-1条件下实现了41.9%的总氮去除率,出水总氮均值为5.8 mg.L-1。     

城市滨海湿地表层沉积物磷形态与相关关系分析

以九龙江口滨海湿地为例,采用连续提取法对沉积物中的磷进行连续提取和测定,探究城市滨海湿地表层沉积物中磷的生物可利用性,同时分析沉积物磷形态以及与环境参数的相关关系。结果表明：总磷（TP）含量为（456.34±13.13）mg·kg-1,生物可利用性磷（Bio-P）占TP59.46%;NaOH-P、HCl-P是TP的主要组成形态;NaOH-P是Bio-P的主要组成形态。相关性研究表明,沉积物中TP含量的增加主要来自NaOH-P,其次是HCl-P。NH4Cl-P、BD-P、NaOH-P、HCl-P之间未呈显著相关,其来源具有差异性。NaOH-P与pH显著负相关,与Fe、Al未呈显著相关;OP与有机质含量亦无显著相关。研究成果将为海域生态环境保护和城市滨海湿地环境恢复提供基础数据。     

三江平原湿地不同土地利用生境地表甲虫群落结构

2007年6月至9月,对三江平原湿地6种不同土地利用生境下地表甲虫群落结构的调查结果显示,所采集的1 646个地表甲虫标本隶属25科,其中步甲、隐翅虫和叩甲3科个体数量较多,分别占总数的52.9%、20.1%和10.8%,共同构成本研究区的优势类群。对该甲虫群落的物种丰富度、多样性指数、均匀度指数的分析结果表明,大豆田地表甲虫数量最多,松树林最少;物种丰富度以白桦-蒙古栎林最高,杨树林最低;多样性指数以田埂最高,杨树林最低;均匀度指数以田埂最高,杨树林最低。土地利用方式对地表甲虫数量、多样性有显著影响,对均匀度无显著影响;季节变化对地表甲虫数量无显著影响,但对地表甲虫多样性和均匀度有显著影响。     

生物法-人工湿地组合工艺对小城镇混合污水氮素去除效果研究

在华南热带亚热带地区,利用生物法-人工湿地组合工艺（A/BCO-CW）处理小城镇片区混合污水。全年运行结果表明,A/BCO-人工湿地组合系统对TN和NH4-N具有好的处理效果,年均去除率分别为57.57%、87.73%,平均去除负荷量达到3.85和3.26g·m-2·d-1。A/BCO预处理系统在停留时间为3～6h条件下,对NH4-N去除效率达57.38%,好氧段充分曝气,有利于硝化过程的进行,有效地解决人工湿地因溶解氧不足造成的硝化过程受限制情况,减少了后续湿地所需面积的25%。在0.30～0.59m·d-1水力负荷率下,垂直流-表面流-水平潜流人工湿地组合系统对TN和NH4-N平均去除负荷达到了2.56、0.90g·m-2·d-1;TN、NH4-N去除速率常数为39.8和41.62m·yr-1,这些数值均处于文献中k值范围的高量程内。在进水NH4-N变动范围为6.27～18.8mg·L-1时,出水水质均能稳定达到GB3838—2002《地表水环境质量标准》的IV类水1.5mg·L-1的标准。通过探讨A/BCO-CW组合工艺用于混合污水处理效率,实现了A/BCO和CW二者间在处理氮素过程中的优势互补。     

天津滨海湿地典型野生盐生植物的应用

天津滨海湿地具有丰富的野生盐生植物资源,其中为数众多的盐生植物成功适应了本地区生境,深入研究其应用对滨海盐碱地改良与植被恢复具有重要意义。在滨海自然盐生植物资源调查的基础上,对滨海盐生植物多样性组成进行了简要分析;结合典型盐生植被景观的构成及特征,重点探究了典型野生盐生植物在滨海盐碱地改良和植被恢复、园林绿化方面的应用。研究表明：本区15科,35余种的野生盐生植物中,能够重点推荐作为盐碱地改良和绿化的典型野生盐生植物至少有15种。最后提出保留和保护典型盐生植物和植被景观的建议。     

EMERGY analysis for sustainability evaluation of the Baiyangdian wetland ecosystem in China

This study presents a quantitative and ecological benefit evaluation of the Baiyangdian wetland in China between the years 2000 and 2006. Methods of EMERGY analysis were applied to illustrate the wetland ecosystem, to evaluate the economic and environmental inputs and consequent yields, and to assess the sustainability of the Baiyangdian wetland. The indicators for the integrated ecological and economic system, such as EMERGY yield ratio (EYR) (7.51), EMERGY investment ratio (EIR) (4.52), environmental loading ratio (ELR) (2.92), EMERGY exchange ratio (0.41), and EMERGY sustainable indicator (ESI) (2.57) were calculated, compared, analyzed, and discussed. The non-renewable investment in Baiyangdian was greater than renewable investment, leading to the unsustainable development of the system. High EYR indicated that the Baiyangdian integrated system had created huge profits for its people, while ELR and ESI revealed that human behavior has been a heavy burden on the environment, and countermeasures should be taken by the Chinese government to relieve and resolve these problems. Potential management methods were also proposed in this paper.