西藏措麦地区新生代火山岩相初步研究

西藏措麦地区处于冈底斯火山—岩浆弧的中段,新生代火山活动强烈,火山岩相发育齐全。根据火山喷发类型、火山物质搬运方式和定位环境,可划分为爆发相、爆溢相、喷溢相、侵出相、潜火山岩相、火山通道相、喷发—沉积相等7大类型。再依据区内火山活动特点,火山相序及相组合特征,结合火山沉积地层及其接触关系,可将其火山活动划分四个喷发—沉积旋回,依序对应于典中组、年波组、帕那组、鱼鳞山组四个岩石地层单位。文章阐述了各类型火山岩相的基本特征,对各火山岩相序、相组合及火山活动旋回作了初步分析和归纳,对提高整个冈底斯地区火山岩研究程度,具有一定的实际意义。     

Bioleaching of spent Ni-Cd batteries and phylogenetic analysis of an acidophilic strain in acidified sludge

Biohydrometallurgy is a novel method to recycle discarded batteries, in which sewage sludge is used as microorganisms and culture due to the presence of indigenous Thiobacilli. A two-step continuous flow leaching system consisting of an acidifying reactor and a leaching reactor was introduced to achieve the bioleaching of spent nickel-cadmium (Ni-Cd) batteries. The acid supernatant produced in the acidifying reactor by the microorganisms with ferrous ions as the substrate was conducted into the leaching reactor to dissolve electrode materials. The efficiency of a batch treatment of batteries was examined. The results showed that the complete dissolution of two AA-sized Ni-Cd batteries with 0.6 L/d acid supernatant took about 30, 20, and 35 days for Ni, Cd, and Co, respectively. But the dissolution ability of the three metals was different. Cd and Co can be leached mostly for pH below 4.0 while the complete dissolution of Ni can be achieved for pH below 2.5. Meanwhile, a strain (named Thiooxidans. WL) accounting for the reduction of pH in the acidified sludge was isolated and sequenced. It was identified to be 100% similar to Acidithiobacillus ferrooxidans strain Tf-49 based on 16S rDNA sequence analysis. The relevant phylogenetic tree constructed indicates that the strain should be classified into genus Acidithiobacillus ferrooxidans.     

宁波盆地白垩系古地磁特征兼论馆头组朝川组和方岩组的层位归属

浙东宁波盆地白垩纪陆相火山岩系,经古地磁研究表明,以往认为时代属于早白垩世早期的馆头组,晚期朝川组和晚白垩世早期的方岩组均呈正向磁化,平均磁化方向基本一致。据绝对年龄和古生物旁证,磁化时间属白垩纪中期正向极性超时。因此,馆头组的层位应作相应的提高,从白垩系下部上升到白垩系中部;朝川组随之位于其上。方岩组纳入白垩系中部,在馆头组之下还应有一套以正负极性交迭为特征的白垩纪早期沉积物。从而对以往所谓的上侏罗统磨石山群的时代提出疑问。从古地磁方向特征的规律,支持了白垩系三分说。     

Interstitial deletion of chromosome 1 [del(1)(q25q32)] in an infant with prune belly sequence

Relatively few cases of deletion 1q have been reported. These cases have been divided into three groups according to assigned breakpoints. They include proximal interstitial, intermediate interstitial, and terminal deletions. We present a male infant with an interstitial deletion of 1q with breakpoints determined by GTG banding as q25 and q32. Comparison with similar case reports suggests common physical features which include microcephaly, growth retardation, developmental delay, clinodactyly, and genital anomalies in affected males. However, no characteristic phenotypic appearance is definable. The infant also presented with prune belly sequence (PBS) with Potter fades. Fetal ascites, as noted in this case on prenatal ultrasound, appears to be an early factor in the pathogenesis of PBS. Therefore, detection of fetal ascites should suggest the presence of the PBS association and the need for more extensive prenatal evaluation.     

天桥铅锌矿床的沉积改造成矿特征

从层序地层、沉积相、矿相剖面、微量元素、稀土元素、铅同位素和包裹体等方面 ,对天桥铅锌矿床及矿床地球化学特征进行了研究 ,认为产于退积型海侵体系域中顺层的似层状铅锌矿体 ,其成因与六盘水裂陷槽演化的某个阶段特定环境有一定联系 ,成矿溶液沿裂陷槽边界断裂上升 ,挤入上覆岩层间隙乃至进入水盆地 ,对围岩发生交代作用 ,参与成岩 -后生作用、多期次的叠加、改造是该矿床的特征     

穿龙薯蓣、黄山药和盾叶薯蓣psbA-trnH片段序列分析

为研究薯蓣叶绿体psbA-trnH片段遗传多样性,探讨该片段用于中药穿龙薯蓣、黄山药和盾叶薯蓣种间分子鉴别和系统学研究中的意义,我们对不同类群薯蓣种的叶绿体psbA-trnH基因间区进行PCR扩增并测序,获得了该区间的完整序列,所得序列用软件MEGA3.0进行相关分析.穿龙薯蓣的psbA-trnH片段全长274bp,黄山药全长279bp,盾叶薯蓣植物个体内的叶绿体DNA则有两种psbA-trnH片段,长度分别为241bp和503bp.经MEGA3.0软件分析,3种薯蓣种间psbA-trnH片段序列的遗传距离(p-distance)为0.00350~0.04545,各个薯蓣种内的不同类群该序列无差异.用UPGMA法根据psbA-trnH序列的遗传距离建立系统发生树,聚类结果和形态分类相符.所得结果显示,psbA-trnH片段序列在所研究的3种薯蓣种内保守,在种间具有明显的较大差异,而3种薯蓣及薯蓣属的系统发生关系尚须进一步研究.图3表2参6     

地衣芽孢杆菌2709和 6816碱性蛋白酶基因在大肠杆菌中的克隆、表达及序列分析

用PCR方法从地衣芽孢杆菌 2 70 9和 6 816中扩增了碱性蛋白酶基因 (apr1和apr2 ) ,扩增的DNA片段插入到大肠杆菌载体 pET -2 8a中 ,构建成重组分泌型表达载体pAPR1、pAPR2 .pAPR1、pAPR2中碱性蛋白酶基因在大肠杆菌宿主JM 10 9(DE3)中得到表达 .SDS -PAGE分析显示融合表达产物的分子量均为 30 .5× 10 3 ,同核酸序列测定所推导的值相符 .表达产物分别占细胞总蛋白的 8.0 %和 7.5 % .2 70 9重组菌所得酶活为 12 10u/mL ,6 816重组菌所得酶活为 1175u/mL .研究发现 ,重组的碱性蛋白酶在进入大肠杆菌周质空间时可能存在前肽自动脱落的现象 .同时 ,对地衣芽孢杆菌 2 70 9碱性蛋白酶基因序列进行了测定和比较分析 ,发现与地衣芽孢杆菌 6 816碱性蛋白酶基因的同源性为 98% .图 5参 11     

rRNA基因ITS区序列分析在粘帚霉属生防菌株种级分类上的应用

对分离自不同地域的粘帚霉属不同种类的生防菌株rRNA基因转录间区进行了克隆测序,用Clustal X软件进行自动排序,用Njplot程序构建系统进化树.供试26个粘帚霉菌株分在4个组,与传统形态分类结果一致,同一种类的不同来源的菌株差异不明显.研究表明,可以根据ITS区DNA序列差异对粘帚霉属进行种级分类,但不能用于区分种内不同地域的菌株之间的差别.图1表1参17     

葡枝根霉(Rhizopus stolonifer)YF6脂肪酶基因的克隆及其序列分析

筛选并鉴定了1株高产脂肪酶菌株葡枝根霉YF6,并采用简并PCR等分子技术从中克隆到1个新的脂肪酶基因lipRs及其对应的全长cDNA,序列分析表明,该基因编码区全长1173bp,不含内含子序列,编码1段由26个氨基酸残基组成的信号肽和1个由365个氨基酸残基组成的成熟蛋白,该成熟蛋白计算分子量(Mr)为39268.27,等电点为pH7.66,有5个可能的N-糖基化位点,含有脂肪酶特征序列GHSLGGA,与来自米根霉(Rhizopus oryzae)的脂肪酶(AAF32408)同源性最高,一致性为83%,相似性为89%.该基因序列已提交GenBank,登录号为DQ139862.     

链格孢菌菌株致病性及其遗传差异性

对分离自紫茎泽兰的19个链格孢菌菌株的致病性进行了比较研究，并对这19个菌株和其它来源的3个菌株的5．8S rDNA及其两侧的ITS1区和ITS2区进行了序列分析．结果显示，链格孢菌菌株的致病性差异明显；21株链格孢菌菌株在5．8SrDNA及其两侧的转录问区（ITS区）的序列无差异，而与百日草链格孢菌（Alternaria zinnine）的序列差异显著，表明21株链格孢菌菌株属种内变异．采用AFLP分子标记技术对21株链格孢菌的DNA扩增片段长度多态性进行了分析，结果表明，链格孢菌自然变异群体内存在很高的遗传多样性．链格孢菌菌株的相似性与菌株的地理来源有一定的相关性，但基于AFLP标记划分的AFLP类群与基于寄主病情指数划分的致病力类群问关系不相一致；起始菌株与变异菌株相似性显著，但二者和致病性的关系不显著．图2表1参18