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81.
细菌是河流生态系统的重要组成部分,在污染物降解、物质循环和能量流动等方面扮演着重要角色.然而亚热带城市河流细菌群落的季节演替和构建机制(确定性和随机性过程)仍不明晰.以流溪河及广州珠江段为研究对象,采用16S rRNA高通量测序分析探讨了不同季节(丰水期和枯水期)水体细菌群落的变化及其构建机制.结果表明:不同季节水体细菌群落的组成存在显著差异,丰水期,变形菌门和异常球菌-栖热菌门的丰度更高;而枯水期,拟杆菌门和厚壁菌门的丰度更高.细菌群落的α和β多样性同样具有显著的季节差异,T、NH4+-N、TOC、pH、EC、DO、NO3--N和DSi是影响细菌群落季节变化的主要环境因子.水体细菌群落的生态网络具有典型的模块化结构,物种间以正相关作用(合作关系)为主,且丰水期物种间的合作关系强于枯水期.随机性过程主导了细菌群落的构建,尤其以扩散限制贡献最大,且细菌群落在枯水期面临的扩散限制要高于丰水期. 相似文献
82.
Isolation and characterization of a strain with high microbial attachment in aerobic granular sludge
Tingting Zhao Kai Qiao Lei Wang Wei Zhang Wei Meng Fan Liu Xu Gao Jianrong Zhu 《环境科学学报(英文版)》2021,33(8):194-203
Aerobic granule is a special microbial aggregate associated with biofilm structure. The formation of aerobic granular sludge is primarily depending on its bacterial community and relevant microbiological properties. In this experiment, a strain with high microbial attachment was isolated from aerobic granular sludge, and the detailed characteristics were examined. Its high attachment ability could reach 2.34 (OD600nm), while other low attachment values were only around 0.06-0.32, which indicated a big variation among the different bacteria. The strain exhibited a very special morphology with many fibric fingers under SEM observation. A distinctive behaviour was to form a spherical particle by themselves, which would be very beneficial for the formation and development of granular sludge. The EPS measurement showed that its PN content was higher than low attachment bacteria, and 3D-EEM confirmed that there were some different components. Based on the 16S rRNA analysis, it was identified to mostly belong to Stenotrophomonas. Its augmentation to particle sludge cultivation demonstrated that the strain could significantly promote the formation of aerobic granule. Conclusively, it was strongly suggested that it might be used as a good and potential model strain or chassis organism for the aerobic granular sludge formation and development. 相似文献
83.
84.
Yanan Bai Xiuning Wang Fang Zhang Raymond Jianxiong Zeng 《Frontiers of Environmental Science & Engineering》2022,16(3):34
85.
Microbial reactions play an important role in regulating pore water chemistry as well as secondary mineral distribution in many subsurface systems and, therefore, may directly impact radionuclide migration in those systems. This paper presents a general modeling approach to couple microbial metabolism, redox chemistry, and radionuclide transport in a subsurface environment. To account for the likely achievement of quasi-steady state biomass accumulations in subsurface environments, a modification to the traditional microbial growth kinetic equation is proposed. The conditions for using biogeochemical models with or without an explicit representation of biomass growth are clarified. Based on the general approach proposed in this paper, the couplings of uranium reactions with biogeochemical processes are incorporated into computer code BIORXNTRN Version 2.0. The code is then used to simulate a subsurface contaminant migration scenario, in which a water flow containing both uranium and a complexing organic ligand is recharged into an oxic carbonate aquifer. The model simulation shows that Mn and Fe oxyhydroxides may vary significantly along a flow path. The simulation also shows that uranium(VI) can be reduced and therefore immobilized in the anoxic zone created by microbial degradation. 相似文献
86.
Yanfeng Yang Ruina Zhang Ziyang Lou 《Frontiers of Environmental Science & Engineering》2022,16(12):158
87.
Shuqin Liu Rui Wu Xi Yang Shuting Fang Zhangmin Xiang Shenghong Yang Gangfeng Ouyang 《Frontiers of Environmental Science & Engineering》2022,16(9):116
88.
Cho ST Tsai SH Ravindran A Selvam A Yang SS 《Environmental geochemistry and health》2008,30(3):255-272
To investigate the seasonal variations of microbial ecology in grassland of Tatachia forest, soil properties, microbial populations,
microbial biomass, and 16S rDNA clone library analysis were determined. The soil had temperatures 6.6–18.4°C, pH 3.6–5.1,
total organic carbon 1.11–10.68%, total nitrogen 0.18–0.78%, and C/N ratios 3.46–20.55. Each gram of dry soil contained bacteria,
actinomycetes, fungi, cellulolytic, phosphate-solubilizing microbes, and nitrogen-fixing microbes 4.54 × 104 to 3.79 × 107, 3.43 × 102 to 2.17 × 105, 5.74 × 103 to 3.76 × 106, 1.97 × 103 to 1.34 × 106, 8.49 × 102 to 5.59 × 105, and 3.86 × 102 to 3.75 × 105 CFU, respectively. Each gram of soil contained 117–2,482 μg of microbial biomass carbon, 23–216 μg of microbial biomass nitrogen
and 9–29 μg of DNA. The microbial populations, microbial biomass, and DNA decreased stepwise with the depth of soil, and they
had low values in winter seasons. The microbial populations, microbial biomass carbon, microbial biomass nitrogen, and DNA
at the BW2 horizon were 8.42–17.84, 19.26–64.40, 16.84–61.11, and 31.03–46.26% of those at the O horizon, respectively. When
analyzing 16S rDNA library, members of Proteobacteria, Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes,
candidate division TM1, candidate division TM7, Gammatimonadetes, and Verrucomicrobia were identified. Members of Proteobacteria
(44.4%) and Acidobacteria (33.3%) dominated the clone libraries. Within the phylum Proteobacteria, α-, β-, and γ-Proteobacteria
were most numerous, followed by δ-Proteobacteria. 相似文献
89.
90.
Jason S. Lee Belinda L. Daniels David T. Eberiel Richard E. Farrell 《Journal of Polymers and the Environment》2000,8(2):81-89
Soil retrieval, processing and storage procedures can have a profound effect on soil microorganisms. In particular, changes in soil microbial populations may adversely affect the biological activity of a soil and drastically alter the soil's potential to mineralize added substrates. The effects of cold storage on the biodegradation of a series of test polymers was investigated using two soils—a synthetic soil mix (SM-L8) and a field soil (Bridgehampton silt loam) from Rhode Island (RI-1). Biodegradation tests were conducted using freshly prepared/collected soil and again following storage at 4°C for 3 to 8 months. Prior to each biodegradation test, the soils were incubated at 60% water-holding capacity (WHC) and 25°C to rejuvenate the microbial populations; the soils were incubated for periods of 48 h (freshly collected soil) or 25 days (soils stored at 4°C). Soil microbial populations were assessed by enumerating different segments of the population on agar plates containing different selective media. Mineralization of the test polymers (cellulose, poly-3-hydroxybutyrate, and starch acetate, d.s. 1.5) was monitored using standard respirometric techniques. Our results demonstrated that cold storage had a generally negative effect on the soil microbial populations themselves but that its effect on the capacity of the soil microorganisms to degrade the test polymers varied between soils and polymer type. Whereas cold storage resulted in dramatic shifts in the community structure of the soil microbial populations, substantial restoration of these populations was possible by first conditioning the soils at 60% WHC and ambient temperatures for 25 days. Likewise, although the effects of cold storage on polymer mineralization varied with the test polymer and soil, these effects could be largely offset by including an initial 25-day stabilization period in the test. 相似文献