Evaluation of the relationship of picoplankton and viruses to environmental variables in a lagoon system (Çakalburnu Lagoon,Turkey)

Lagoons are defined as wetlands separated from the sea coasts on which they are located and sit between continental and marine aquatic ecosystems. Çakalburnu Lagoon is a coastal wetland on the southern side of the Bay of ?zmir. Microorganisms, which are quite sensitive to changes occurring in environmental factors, are commonly used to determine the impact of environmental effects on the functioning of the ecosystem. In this study, variations in the abundance and biomass of picoplankton (Synechococcus spp. and bacteria) and the abundance of viruses, which identify the ecological productivity of the food chain, were seasonally examined by epifluorescence microscopy. Moreover, the microbial abundance and biomass relation over time between the physical and chemical parameters was evaluated. According to our results, the maximum abundance of Synechococcus spp. and viruses was 6.7?×?10⁴ cell/ml and 9.9?×?10⁸?cell/ml in the summer, respectively. Otherwise, the highest level of bacteria was measured at 3.6?×?10⁷ cell/ml in the spring. Based on the principal component analysis and Pearson correlation analysis results, we concluded that total suspended solids, Chl-a, particulate organic carbon and particulate organic nitrogen were the major parameters influencing the observed variability of the lagoon system. Overall, to protect and improve the ecological and microbiological quality of aquatic systems such as lagoons, the necessary monitoring and measurement studies should be conducted in these sensitive areas.     

Roles of acid-producing bacteria in anaerobic digestion of waste activated sludge

Three acid-producing strains, AFB-1, AFB-2 and AFB-3, were isolated during this study, and their roles in anaerobic digestion of waste activated sludge (WAS) were evaluated. Data of 16S rRNA method showed that AFB-1 and AFB-2 were Bacillus coagulans, and AFB-3 was Escherichia coli. The removal in terms of volatile solids (VS) and total chemical oxygen demand (TCOD) was maximized at 42.7% and 44.7% by inoculating Bacillus coagulans AFB-1. Besides, the optimal inoculum concentration of Bacillus coagulans AFB-1 was 30% (v/v). Solubilization degree experiments indicated that solubilization ratios (SR) of WAS reached 20.8%±2.2%, 17.7%±1.48%, and 11.1%±1.53%. Volatile fatty acids (VFAs) concentrations and compositions were also explored with a gas chromatograph. The results showed that VFAs improved by 98.5%, 53.0% and 11.6% than those of the control, respectively. Biochemical methane potential (BMP) experiments revealed that biogas production increased by 90.7% and 75.3% when inoculating with Bacillus coagulans AFB-1 and AFB-2. These results confirmed that the isolated acid-producing bacteria, especially Bacillus coagulans, was a good candidate for anaerobic digestion of WAS.

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Effects of water velocity on bulk water quality and biofilm population structure in drinking water distribution systems北大核心CSCD

To study the effect of flow velocity on drinking water distribution systems, bulk water quality was monitored over 28 days, biomass was measured, and 16S rDNA was sequenced on the 28th day using a water distribution simulation system. The relationship between bulk water quality and biofilm was statistically analyzed. Flow velocity of 0.5 m/s yielded the most total organic carbon (TOC) (5.26 ± 0.17 mg/L) in the bulk water, the most bulk water bacteria (lg (n+1/mL-1) = 4.79 ± 0.02), the worst bulk water quality, and the most biofilm bacteria (lg (n+1/cm-2) = 5.48 ± 0.06). A Pearson correlation analysis showed the total number of biofilm bacteria was positively correlated with conductivity (R = 0.73, P < 0.01), turbidity (R = 0.87, P < 0.001), TOC (R = 0.94, P < 0.001), and total bacteria (R = 0.92, P < 0.001), and was negatively correlated with residual chlorine (R = -0.68, P < 0.05). Biofilm diversity was high under the low (0.1 m/s) and high (2.5 m/s) flow rates, but the bacterial diversity of biofilm was the lowest at the 0.5 m/s flow rate, in which Proteobacteria dominated the biofilm community structure. These results suggest that flow velocity affects bulk water quality and biofilm population structure, and water quality and biofilm population structure are interrelated, which provides the theoretical basis for research on biofilms in drinking water distribution systems. © 2018 Science Press. All rights reserved.     

Mercury biomethylation assessment in the estuary of Bilbao (North of Spain)

The relationship between the microbial methylation of mercury and the microbial activities in sediments and water collected from the estuary of Bilbao (North of Spain) was studied in three different sampling points and in two different seasons. Three different cultures were prepared with a sediment slurry to distinguish between biotic and abiotic methylation pathways and the variations of the methylmercury concentration and the variations of the population of total number of bacteria (TDC), anaerobic heterotrophic bacteria (AHB), sulphate-reducing bacteria (SRB) and Desulfovibrio were measured. From this work, it can be concluded that the variation of MeHg concentrations is a result of the methylation/demethylation processes in the sediments, and that the abiotic processes have a negligible contribution to those processes. According to the statistical analysis of the results (partial least squares analysis) a significant statistical correlation was established between methylmercury and the SRB counts.     

Effect of salinity on community structure and naphthalene dioxygenase gene diversity of a halophilic bacterial consortium

The aim of this study is to analyze the effect of salinity on polycyclic aromatic hydrocarbons (PAHs) biodegradation, community structure and naphthalene dioxygenase gene (ndo) diversity of a halophilic bacterial consortium with the denaturing gradient gel electrophoresis (DGGE) approach. The consortium was developed from oil-contaminated saline soil after enrichment for six times, using phenanthrene as the substrate. The prominent species in the bacterial consortium at all salinities were identified as halophilic bacteria Halomonas, Alcanivorax, Marinobacter, Idiomarina, Martelella and uncultured bacteria. The predominant microbes gradually changed associating with the saline concentration fluctuations ranging from 0.1% to 25% (w/v). Two ndo alpha subunits were dominant at salinities ranging from 0.1% to 20%, while not been clearly detected at 25% salinity. Consistently, the biodegradation occurred at salinities ranging from 0.1% to 20%, while no at 25% salinity, suggesting the two ndo genes played an important role in the degradation. The phylogenetic analysis revealed that both of the two ndo alpha subunits were related to the classic nah-like gene from Pseudomonas stutzeri AN10 and Pseudomonas aeruginosa PaK1, while one with identity of about 82% and the other one with identity of 90% at amino acid sequence level. We concluded that salinity greatly affected halophilic bacterial community structure and also the functional genes which were more related to biodegradation.

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冶炼企业周边农田土壤的多环芳烃污染及其细菌群落效应

多环芳烃是一类持久性有机污染物,进入土壤后可能产生多方面生态效应。为研究多环芳烃对土壤微生物的影响,选取南京某冶炼企业周边农田样品,在分析污染物含量基础上,采用高通量测序、定量PCR等方法综合评价了土壤细菌多样性和组成以及多环芳烃降解细菌丰度等特征。17个土壤样品中,多环芳烃总量为0.25~31.08 mg·kg-1,并具有随污染源距离增加而降低的空间分布特征。与土壤理化性质如p H相比较,多环芳烃污染对土壤细菌的总体多样性和群落组成影响不显著。进一步分析发现多环芳烃与潜在降解微生物的相对丰度和降解功能基因(芳香环羟基化双加氧酶,PAH-RHDα)拷贝数显著正相关。污染较重样品的克隆、测序分析表明,土壤中PAH-RHDα基因主要属于革兰氏阳性细菌nid A3/fad A1类群,且与分支杆菌相关序列较为接近。这些结果综合评价了冶炼企业周边农田土壤多环芳烃污染对微生物群落的影响,提示土壤污染在多环芳烃潜在降解细菌中的富集作用,将为后续污染土壤生物修复提供重要科学依据。     

Microprofiles of activated sludge aggregates using microelectrodes in completely autotrophic nitrogen removal over nitrite (CANON) reactor

Microsensor measurements and fluorescence in situ hybridization (FISH) analysis were combined to investigate the microbial populations and activities in a laboratory-scale sequencing batch reactor (SBR) for completely autotrophic nitrogen removal over nitrite (CANON). Fed with synthetic wastewater rich in ammonia, the SBR removed 82.5±5.4% of influent nitrogen and a maximum nitrogen-removal rate of 0.52 kgN·m⁻³·d⁻¹ was achieved. The FISH analysis revealed that aerobic ammonium-oxidizing bacteria (AerAOB) Nitrosomonas and anaerobic ammonium-oxidizing bacteria (AnAOB) dominated the community. To quantify the microbial activities inside the sludge aggregates, microprofiles were measured using pH, dissolved oxygen (DO), NH4+, NO2− and NO3− microelectrodes. In the outer layer of sludge aggregates (0–700 μm), nitrite-oxidizing bacteria (NOB) showed high activity with 4.1 μmol·cm⁻³·h⁻¹ of maximum nitrate production rate under the condition of DO concentration higher than 3.3 mg·L⁻¹. Maximum AerAOB activity was detected in the middle layer (depths around 1700 μm) where DO concentration was 1.1 mg·L⁻¹. In the inner layer (2200–3500 μm), where DO concentration was below 0.9 mg·L⁻¹, AnAOB activity was detected. We thus showed that information obtained from microscopic views can be helpful in optimizing the SBR performance.     

生物除磷机理及试验研究

介绍了生物除磷机理、生物除磷系统的积磷细菌、生物除磷所需的环境条件。SBR法除磷的试验研究表明,应用SBR活性污泥系统进行除磷,需要一定的厌氧条件;且须在厌氧阶段进行充足的曝气。试验结果证明,SBR法在时间上控制的灵活性非常适合生物除磷的环境条件。厌氧与好氧相结合,能够提高磷的去除率或降低出水磷的浓度,磷的去除率可达90%左右。     

光合细菌和螺旋藻对黄泔水净化与利用初探

应用光合细菌将黄泔水中的有机物降解转化为无机氮磷后养殖螺旋藻。结果表明,在实验条件下光合细菌对黄泔水ＣＯＤｃｒ的去除率可达７０％,处理后的黄泔水用甲壳质絮凝澄清后加入螺旋藻并曝气进行培养,藻生长周期为２２ｄ,生长速率为２０－３０ｍｇ／Ｌ．Ｄ。藻体蛋白质含量平均为５２．６ｇ／１００ｇ游离氨基酸平均含量为２．４ｇ／１００ｇ。     

胶州湾潮间带和沿岸区硫酸盐还原菌含量分布

用 Ｍ Ｐ Ｎ 法对胶州湾潮间带和沿岸区的硫酸盐还原菌（ 缩写为 Ｓ Ｒ Ｂ） 含量作了研究，结果表明表层沉积物的 Ｓ Ｒ Ｂ 含量大约是４１ ×１０７ 个细胞／ｇ ，波动范围较大。水中的 Ｓ Ｒ Ｂ 含量约２５ ×１０２／ｍｌ。分析了 Ｓ Ｒ Ｂ 含量的分布状况，发现调查区内的 Ｓ Ｒ Ｂ 含量基本上呈现出北高南低、西高东低之势，即工业区、港口区的高于海水浴场、海滨旅游区的。证实了生活废弃物的排放致其环境 Ｓ Ｒ Ｂ 含量特高。 Ｓ Ｒ Ｂ 含量分布状况大体上与以往所测环境污染状况一致。对虾养殖池的 Ｓ Ｒ Ｂ 含量意味着水产养殖必须考虑这一因子的作用