蟒蛇河水域浮游植物调查与水生态评价

2012—2013年春(3月)、秋(9月)两季,从蟒蛇河上游大纵湖至下游新洋港大桥,共设置6个监测点位,调查浮游植物种类组成及群落结构,并利用群落结构指数等方法评价其水生态状况,结果表明:蟒蛇河水域共监测到浮游植物64种,以硅藻门、绿藻门和蓝藻门为主要门类;各监测点位浮游植物密度呈现秋季显著高于春季的季节性变化趋势;春、秋季节浮游植物优势种存在交叉演替的现象;蟒蛇河浮游植物多样性较好,指征水体污染程度为尚清洁;Shannon-wiener多样性指数、Margalef物种丰富度指数和Pielou均匀度指数数值均表现为春季高于秋季,且上、下游点位间差异不明显。     

地表水浮游植物叶绿素a测定方法比较研究

比较了浮游植物叶绿素a测定方法中的丙酮萃取法和热乙醇萃取法.结果表明,应用反复冻融植物细胞的丙酮萃取法对地表水中浮游植物叶绿素a的萃取率显著高于其他方法,且重复测定的精密度较高.     

连云港港区海洋环境质量状况

为分析连云港海洋环境质量,对连云港港区水质、沉积物及生物因子进行监测。结果表明,港区内某些点位水质中无机氮含量较高,达到污染程度,其余点位监测指标均正常,沉积物中的重金属尚未对该海域造成污染。浮游植物31种,其中甲藻门8种,硅藻门23种,浮游植物数量变动为1.84×104m-3~1.16×105m-3;底栖生物17种,多样性指数平均值为1.89。港区内水域环境时空变化表明,海水中锌含量连续大幅增加,其余指标均下降50%,沉积物重金属含量变化不大。     

江阴市主要河流夏季浮游藻类的群落结构及多样性

2011年6—8月采集江阴市6条河流的浮游藻类样品,分析其群落结构及物种多样性,并利用多样性指数、指示性类群和硅藻商对水质进行了生物学评价。共检出浮游藻类6门137属种,平均生物量2.38 mg/L,蓝藻、绿藻、硅藻分别占72.3%、10.2%和8.41%。Shannon-Wiener多样性指数、Margalef丰富度指数和Pielou均匀度指数的变幅分别为3.34~3.59、4.75~6.40和0.73~0.75,硅藻商变幅为8.3~16.4。多样性指数评价结果表明,水体处于轻或无污染状态;指示性浮游植物类群和硅藻商评价结果表明,河流处于α中污带-β中污滞-多污滞。相关分析表明,Shannon-Wiener多样性指数与理化水质因子不显著相关,与悬浮物SS显著负相关,与总有机碳显著正相关。研究认为,指示性浮游植物类群或硅藻商更适于作为城市河流水质评价的生物指标。     

Influence of Mining Activities in the North of Potosi, Bolivia on the Water Quality of the Chayanta River, and its Consequences

Mining activity in the North of Potosi (Siglo XX mine, Ingenio Catavi-Siglo XX, Pucro mine and Colquechaca mine) produces minewater containing high concentrations of heavy metals such as As (0.02-34 mg/l), Cd (45-11,600 microg/l), Cu (0.35-32 mg/l), Fe (42-1,010 mg/l), Pb(33-3,130 microg/l), Ni(20-4,320 microg/l), and Zn (1.1-485 mg/l), that exceed considerably the limit values. The rivers in the North of Potosi (Katiri and Pongoma) that do not receive minewater contain clear water with rather low heavy metal concentrations. These rivers and also other rivers contaminated with minewater, are tributaries of the Chayanta River that transports water with a high concentration of heavy metals such as As (6-24 microg/l), Cd (260-2,620 microg/l), Cu (205-812 microg/l), Pb(10-21 microg/l) and Ni(110-332 microg/l). These elements result from mining activity, as indicated by a comparison with rivers not contaminated by minewater discharges. Water of the Chayanta River, used all year long by the population of Quila Quila, (a village situated at about 75 km from the mining centers), for the irrigation of crops such as potato, maize and broad bean, contains heavy metal concentrations exceeding for several elements the guidelines for irrigation. As drinking water the population of Quila Quila consumes spring water with a generally acceptable heavy metal concentration, as well as infiltrated water of Chayanta River (which is also used in animal drinking troughs) with a high concentration of Cd (23-63 microg/l), exceeding the limit value for drinking water. The metal concentration is significantly lower in the infiltrated water than in the water of Chayanta River. Some technological solutions are suggested to improve the quality of the water used. Surveys carried out on inhabitants of the region, showed that many people present health problems, probably to be attributed to the bad quality of the water they consume and use for irrigation.     

无机碳对浮游藻类生长和群落结构的影响

采用水族箱生态模拟方法,利用碳酸钠和碳酸氢钠作为碱度调节剂设置不同碱度水平,研究了氮磷营养相对充足的试验水体中藻类各相关指标对无机碳的响应,并对试验过程中所测得的理化指标和生物指标进行统计分析.研究表明:富营养水体无机碳含量与藻类叶绿素水平存在着正相关关系;适当增加水体中的无机碳浓度能够改变蓝、绿藻门种类比例;当碱度水平达到一定程度时,铜绿微囊藻可以逐渐成为优势种,并从体系中上升,演替成为水华.     

Light-limited population dynamics of phytoplankton: modeling light and depth effects

Light has received very less attention as a selective factor, probably because the changing vertical light gradient makes light a rather complex factor when compared with the nutrient competition. In our study we modified an analytically tractable model based upon the Beer–Lambert law. The study aimed to predict the outcome of phytoplankton competition for light, on the basis of field measurements. When we considered competition theory, Fragilaria was found to be more competitive than other genera. The overall result shows that the trends flow in the following manner; Fragilaria > Gomphonema > Diatoma > Amphora > Achnanthes.     

Cell size dependence of additive versus synergetic effects of UV radiation and PAHs on oceanic phytoplankton

Polycyclic Aromatic Hydrocarbons’ (PAHs) toxicity is enhanced by the presence of ultraviolet radiation (UVR), which levels have arisen due to the thinning of the ozone layer. In this study, PAHs’ phototoxicity for natural marine phytoplankton was tested. Different concentrations of a mixture of 16 PAHs were added to natural phytoplankton communities from the Mediterranean Sea, Atlantic, Arctic and Southern Oceans and exposed to natural sunlight received in situ, including treatments where the UVR bands were removed. PAHs’ toxicity was observed for all the phytoplankton groups studied in all the waters and treatments tested, but only for the pico-sized group a synergetic effect of the mixture and UVR was observed (p = 0.009). When comparing phototoxicity in phytoplankton from oligotrophic and eutrophic waters, synergy was only observed at the oligotrophic communities (p = 0.02) where pico-sized phytoplankton dominated. The degree of sensitivity was related to the trophic degree, decreasing as Chlorophyll a concentration increased.     

Generation of reactive oxygen species in cyanobacteria and green algae induced by allelochemicals of submerged macrophytes

Inhibition of phytoplankton by allelochemicals released by submerged macrophytes is reported to be one of the mechanisms that maintain a clear-water state in shallow lakes. In order to elucidate this mechanism, the ability of six polyphenols and two long-chain fatty acids to induce the generation of reactive oxygen species (ROS) in phytoplankton was studied using the ROS sensitive probe 2′,7′- dichlorodihydrofluorescein diacetate (DCFH-DA). The results showed that only (+)-catechin (CA) and pyrogallic acid (PA) could induce ROS formation in Microcystis aeruginosa and Pseudokirchneriella subcapitata. 25 mg L⁻¹ CA caused 1.2, 1.4 and 1.8 times increase of ROS levels in M. aeruginosa at 1, 2 and 4 h exposure, respectively, and, correspondingly in P. subcapitata cells, these values were 3.7, 6.2 and 7.7, respectively. PA also significantly increased the levels of intracellular ROS in P. subcapitata (P < 0.01); however, significant ROS generation in M. aeruginosa was observed at only 4 h exposure (P < 0.01). Light enhanced ROS generation in CA treated cells, but not in the cells treated with PA. CA and PA may act as redox cyclers after uptake by test organisms and produce ROS successively. These results suggest that the oxidative stress induced by the redox cycling property of allelochemicals may be one of the important causes for the inhibitory effect of some submerged macrophytes towards undesired phytoplankton in natural aquatic ecosystems.     

长江口大洋山近岸水域浮游植物群落及其与环境因子的关系

2012年对上海大洋山近岸水域浮游植物的群落结构和物种多样性的周年动态进行了逐月的研究。结果表明:浮游植物共有141种,隶属6门62属。以硅藻为主,共43属110种,占物种总种类的78.01%。该水域浮游植物的年平均细胞密度为(37.86±39.79)×104cell/L。中肋骨条藻(Skeletonema costatum)和具槽帕拉藻(Paralia sulcata)为第一和第二优势种,细长列海链藻(Thalassiosira leptopus)、肋缝菱形藻(Nitzschia frustulum)和梅尼小环藻(Cyclotella meneghiniana)为该水域常见种。从水质参数和物种多样性指数综合评价:大洋山近岸水域水质总体属于轻中污染。冗余分析(RDA)表明对大洋山近岸水域浮游植物影响较大的是悬浮物浓度、盐度、水温和溶解氧。