Genetic Identification of Spotted Owls, Barred Owls, and Their Hybrids: Legal Implications of Hybrid Identity

Abstract:  Recent population expansion of Barred Owls (  Strix varia ) into western North America has led to concern that they may compete with and further harm the Northern Spotted Owl (  S. occidentalis caurina ), which is already listed as threatened under the U.S. Endangered Species Act (ESA). Because they hybridize, there is a legal need under the ESA for forensic identification of both species and their hybrids. We used mitochondrial control-region DNA and amplified fragment-length polymorphism (AFLP) analyses to assess maternal and biparental gene flow in this hybridization process. Mitochondrial DNA sequences (524 base pairs) indicated large divergence between Barred and Spotted Owls (13.9%). Further, the species formed two distinct clades with no signs of previous introgression. Fourteen diagnostic AFLP bands also indicated extensive divergence between the species, including markers differentiating them. Principal coordinate analyses and assignment tests clearly supported this differentiation. We found that hybrids had unique genetic combinations, including AFLP markers from both parental species, and identified known hybrids as well as potential hybrids with unclear taxonomic status. Our analyses corroborated the findings of extensive field studies that most hybrids genetically sampled resulted from crosses between female Barred Owls and male Spotted Owls. These genetic markers make it possible to clearly identify these species as well as hybrids and can now be used for research, conservation, and law enforcement. Several legal avenues may facilitate future conservation of Spotted Owls and other ESA-listed species that hybridize, including the ESA similarity-of-appearance clause (section 4[e]) and the Migratory Bird Treaty Act. The Migratory Bird Treaty Act appears to be the most useful route at this time.     

Zooplankton proportion estimates from non-uniform sample volumes

The relative abundance of organisms from different taxa provides information about ecosystem health and diversity. When the numbers of sampled organisms are modelled as Poisson counts, and the sample volumes are not uniform, variance for the proportion attributable to each taxon is difficult to compute. We present a method for computing approximate variances for this situation. The point estimates and their standard errors reduce to the standard multinomial maximum likelihood results when sample volumes are uniform. Further, given initial estimates of population densities for the taxa of interest, optimal sample volumes can be computed. The methods are illustrated for zooplankton counts from Andrus Lake, Michigan.     

Interactions betweenLythrum salicaria and native organisms: A critical review

Seventy-one articles concerningLythrum salicaria (purple loosestrife), a European herb introduced to North America, were reviewed for evidence of utilization by North American fauna and the effect of loosestrife on native plant species. In contrast to popular claims, 29 species of organisms were found to utilize this plant, and no evidence of species declines due to purple loosestrife were found. Evidence that loosestrife out-competes cattails and other plant species was found to be lacking or contradictory. Thus detailed, quantitative data are needed to understand loosestrife's natural history, population dynamics, and impacts on native ecosystems if we are to effectively manage this plant.     

Scale-dependent inconsistencies in the effects of trampling on a forest understory community

The response of forest understory vegetation to trampling applied at different temporal and spatial scales was determined in a cliff-edge forest in Ontario, Canada. Three frequencies (0, 50, 500 passes per year) of short-term trampling (one year) were applied to plots previously undisturbed. Existing trails that had received three frequencies (approx. 100, 500, 25,000 passes per year) of long-term trampling (18 years) were also studied. Community composition, species richness, and individual species frequency were recorded in plots within 4 m and (or) 1 m of the patch centerline. The quantitative and qualitative form of plant response to increased trampling was compared for short-term and long-term treatments, both within 4 m and within 1 m of the path centerline, to judge the consistency of trampling effects at different temporal and spatial scales. As trampling frequency increased, community composition changed progressively, but consistently, in plots both within 4 m and 1 m of the path centerline. Species richness was less affected by trampling and only decreased within 1 m of the path centerline at the highest level of trampling (25,000 passes per season for 18 years). Effects of trampling on individual species frequency were much less consistent at different temporal and spatial scales of trampling. The scale-dependence results suggest that field workers and resource managers both should try explicitly to include and define multiple scale components when trying to ascertain the response of vegetation to human disturbance factors.     

Temporal reproductive isolation and gametic compatibility are evolutionary mechanisms in the<Emphasis Type="Italic"> Acropora humilis</Emphasis> species group (Cnidaria; Scleractinia)

Patterns of interbreeding between individuals are fundamental to the structure and maintenance of evolutionary boundaries between species. In corals, both hybridisation and reproductive isolation appear to be important evolutionary mechanisms. In this study, I examine evolutionary boundaries using morphological, molecular and reproductive criteria within the Acropora humilis species group at Lizard Island on the Great Barrier Reef, Australia. Five species and seven morphs are recognised on the basis of morphological appearance of features traditionally used to identify corals of the genus Acropora. In a molecular phylogenetic analysis, I examine relationships for the mitochondrial DNAs putative control region, using maximum-parsimony and maximum-likelihood methods. The reproductive criteria explore whether species and morphs are reproductively isolated on the basis of temporal or fertilisation barriers. Timing of gamete maturity is surveyed for each species and morph, from the month prior to and 3 months after the mass spawning. Time of spawning is documented at the levels of night and hour of spawning, and time taken for egg-sperm bundles to separate. Laboratory fertilisation experiments tested the potential of species and morphs to interbreed. High levels of intraspecific and extremely low or zero fertilisation levels between the five species indicated that they are valid species. Based on the combined assessment of morphological, molecular and reproductive criteria, A. humilis and A. gemmifera appear to be the most closely related species, which are most closely related to the remaining species in the following order: A. samoensis, A. monticulosa and A. digitifera. Evidence derived from one or more of these criteria suggest that the morphs (1) are at various stages of divergence from the species with which they share morphological characters, and (2) may indicate possible zones of speciation and hybridisation. Identification of morphs avoided the possibility of taxonomic error and was essential for accurate interpretation of evolutionary boundaries. Confirmation of morphology as an informative character of evolutionary boundaries is of great significance because most coral research projects rely on morphology as the primary tool for identification of species.Communicated by M.S. Johnson, Crawley     

Accounting for Uncertainty in Making Species Protection Decisions

Abstract:  Uncertainty gives rise to two decision errors in implementing the U.S. Endangered Species Act: listing species that are not in danger of extinction and delisting species that are in danger of extinction. I evaluated four methods (minimum standard, precautionary principle, minimax regret criterion, adaptive management) for deciding whether to list or delist a species when there is uncertainty about how those decisions are likely to influence survival of the species. A safe minimum standard criterion preserves some minimum amount or safe standard (population) of a species unless maintaining that amount generates unacceptable social cost. The precautionary principle favors not delisting a species when there is insufficient evidence on the efficacy of state management plans for protecting them. A minimax regret criterion selects the delisting decision that minimizes the maximum loss likely to occur under alternative ecosystem states. When the cost of making a correct decision is less than the cost of making an incorrect decision, the minimax regret criteria indicates that delisting is the optimal decision. Active adaptive management employs statistically valid experiments to test hypotheses about the likely impacts of delisting decisions. Safe minimum standard and minimax regret criterion are not compatible with the U.S. Endangered Species Act. The precautionary principle comes closest to describing how federal agencies make delisting decisions. Active adaptive management is scientifically superior to the other methods but is costly and time consuming and may not be compatible with the U.S. National Environmental Policy Act.     

Laying the foundation for a comprehensive program of restoration for wildlife habitat in a riparian floodplain

We analyzed the past and current distribution and abundance of vegetation and wildlife to develop a wildlife habitat restoration plan for the Sweetwater Regional Park, San Diego County, California. Overall, there has been a substantial loss of native amphibians and reptiles, including four amphibians, three lizards, and 11 snake species. The small-mammal community was depauperate and dominated by the exotic house mouse (Mus musculus) and the native western harvest mouse (Reithrodontomys megalotis). It appeared that either house mice are exerting a negative influence on most native species or that they are responding positively to habitat degradation. There has apparently been a net loss of 13 mammal species, including nine insectivores and rodents, a rabbit, and three large mammals. Willow (Salix) cover and density and cottonwoods (Populus fremontii) had the highest number of positive correlations with bird abundance. There has been an overall net loss of 12 breeding bird species; this includes an absolute loss of 18 species and a gain of six species. A restoration plan is described that provides for creation and maintenance of willow riparian, riparian woodland, and coastal sage scrub vegetation types; guides for separation of human activities and wildlife habitats; and management of feral and exotic species of plants and animals.     

预防外来物种入侵的立法对策

对我国目前预防外来物种入侵问题的现状进行了分析,指出了我国在预防外来物种入侵立法上没有专门的针对性法律,立法目的有偏差、法律涉及范围有限的弊病,提出了明确立法目的、制订专项立法与中长期规划、适当扩大相关法律覆盖范围、完善法律法规和顺应国际形势的立法对策.     

基于MATLAB的湘江流域工业固体废物灰色预测

对灰色预测原理及其建模步骤作了系统介绍。借助MATLAB软件建立等维灰数递补残差修正动态模型预测湘江流域未来10年工业固体废物年产量,以期为湘江流域今后工业固体废物的管理、污染防治工作提供科学依据。结果表明:(1)用此模型进行预测,相对误差均在5%以下,预测精度较高,取得了较理想的预测效果;(2)湘江流域未来10年工业固体废物年产量预测值呈指数趋势增长,年增长率为8%～12%,到2016年湘江流域工业固体废物年产量预计将达到7645.01万t。