硫酸厂淤泥中SRB的选育及特性研究

从硫酸厂废水淤泥中分离筛选出了一株 SRB,其菌体形态呈弧状或细杆状 ,成熟细胞大小 0 .4～ 0 .6× 3 .0～ 5 .0μm,摇摆式运动 ,单极生鞭毛 ,芽孢染色阴性 ,革兰氏染色阴性 ;最适生长 p H6.0～ 6,5 ;适量氧气不会影响细菌生长 ,菌体内含过氧化氢酶。本文还探讨了该类细菌的分离培养方法 ,考察了分离菌部分生理生化特征及对硫酸盐还原能力 ,优化了培养基配方 ,并初步鉴定分离菌为脱硫弧菌     

Characterization of sulfide oxidation and optimization of sulfate production by a thermophilic Paenibacillus naphthalenovorans LYH-3 isolated from sewage sludge composting

The sulfur-containing odor emitted from sludge composting could be controlled by sulfide oxidizing bacteria, yet mesophilic strains show inactivation during the thermophilic stage of composting. Aimed to investigate and characterize the thermotolerant bacterium that could oxidize sulfide into sulfate, a heterotrophic strain was isolated from sewage sludge composting and identified as Paenibacillus naphthalenovorans LYH-3. The effects of various environmental factors on sulfide oxidation capacities were studied to optimize the sulfate production, and the highest production rate (27.35% ± 0.86%) was obtained at pH 7.34, the rotation speed of 161.14 r/min, and the inoculation amount of 5.83% by employing Box-Behnken design. The results of serial sulfide substrates experiments indicated that strain LYH-3 could survive up to 400 mg/L of sulfide with the highest sulfide removal rate (88.79% ± 0.35%) obtained at 50 mg/L of sulfide. Growth kinetic analysis presented the maximum specific growth rate µ_m (0.5274 hr⁻¹) after 22 hr cultivation at 50°C. The highest enzyme activities of sulfide quinone oxidoreductase (0.369 ± 0.052 U/mg) and sulfur dioxygenase (0.255 ± 0.014 U/mg) were both obtained at 40°C, and the highest enzyme activity of sulfite acceptor oxidoreductase (1.302 ± 0.035 U/mg) was assessed at 50°C. The results indicated that P. naphthalenovorans possessed a rapid growth rate and efficient sulfide oxidation capacities under thermophilic conditions, promising a potential application in controlling sulfur-containing odors during the thermophilic stage of sludge composting.     

利用光合细菌处理养猪场污水的试验研究

采用煤渣作为填料 ,光合细菌培养物作为挂膜菌的生物接触氧化法 ,对养猪场污水处理进行了试验研究 ,结果表明 :CODCr,NH3 N ,NO-2 N ,S2 - 的平均去除率分别达到 89 3% ,80 1 % ,90 6% ,82 4% ;水中光合细菌数从580个 /mL增加到 4 8× 1 0 4 个 /mL ,达到了废水资源化的目的。     

生物除磷机理及试验研究

介绍了生物除磷机理、生物除磷系统的积磷细菌、生物除磷所需的环境条件。SBR法除磷的试验研究表明,应用SBR活性污泥系统进行除磷,需要一定的厌氧条件;且须在厌氧阶段进行充足的曝气。试验结果证明,SBR法在时间上控制的灵活性非常适合生物除磷的环境条件。厌氧与好氧相结合,能够提高磷的去除率或降低出水磷的浓度,磷的去除率可达90%左右。     

天然林保护与东北地区水患治理方略

从天然林水文功能出发，阐述了东北地区天然林削减及其与洪涝灾害的关系，提出了东北地区水患治理方略。同时建议建立防汛信息系统，提高暴雨和洪水预报准确率以及防洪保险灾后重建。     

Distribution characteristics of ammonia-oxidizing bacteria in the Typha latifolia constructed wetlands using fluorescent in situ hybridization(FISH)

A molecular biology method, fluorescent in situ hybridization（FISH）, in which the pre-treatment was improved in allusion to the media of the constructed wetlands（CW）, e.g. the soil and the grit, was used to investigate the vertical distribution characteristics of ammonia-oxidizing bacteria（AOB） quantity and the relation with oxidation-reduction potential（ORP） in the Typha latifolia constructed wetlands under three different Ioadings in summer from May to September. Results showed that the quantity of the AOB decreased in the Typha latifolia CW with the increase of vertical depth. However, the AOB quantity was 2-4 times the quantity of the control in the root area. Additionally, ORP in the rhizosphere was found to be higher than other areas, which showed that Typha latifolia CW was in an aerobic state in summer when using simulated non-point sewage at the rural area of Taihu Lake in China and small town combined sewage.     

Genetic amniocentesis following multifetal pregnancy reduction to twins: Assessing the risk

Fifty-three patients who elected to reduce their pregnancies to a twin gestation in our centre are known to have subsequently undergone genetic amniocentesis. Five of these patients lost their entire pregnancy following the genetic amniocentesis procedure. This is equivalent to a 9·4 per cent pregnancy loss rate for reduced twin gestations in comparison with an expected loss rate of 2 per cent for non-reduced twin gestations.     

水解酸化—厌氧工艺处理高浓度抗生素废水研究

研究了水解酸化与厌氧消化组合工艺处理高浓度抗生素废水,结果表明,水解酸化反应器最大CODcr容积负荷达到 16.84kg/m3·d,复合厌氧反应器CODcr容积负荷达到8.57kg/m3·d;系统进水SO42-浓度为1325mg/L,CODcr/SO42-值最低达到 3;CODCr与SO42-总去除率分别为75.5％和95.2％,对各种抑制物质和冲击负荷表现出很好的适应性。     

First-trimester prenatal diagnosis in quintuplets: A practical approach using step-by-step embryo reduction

A multiple pregnancy of high rank may occur in a couple at risk for a Mendelian disorder. Prenatal diagnosis is hampered by the difficulty of (1) obtaining chorionic villi from each zygote arid (2) unequivocally relating each sample to the corresponding embryo. The calculation of the genetic risk according to the number of zygotes led us to propose a diagnostic strategy based on embryo reduction, a technique initially designed to improve the perinatal outcome of multiple pregnancies with normal embryos. We report a case in which this approach allowed rational use of first-trimester chorionic villus sampling in a quintuplet pregnancy at risk for non-ketotic hyperglycinaemia, resulting in the selective birth of unaffected twins.     

表面活性剂对分枝杆菌KR2生长的影响

研究了表面活性剂的浓度、离子类型和分子结构等对分枝杆菌KR2菌株生长的影响。结果表明，一些表面活性剂在低浓度时可以促进分枝杆菌KR2的生长，高浓度时则会抑制KR2菌株的生长。表面活性剂的离子类型对KR2菌株生长抑制作用的顺序为：阳离子活性剂TDTMA＞阴离子表面活性剂LAS＞非离子表面活性剂Tween80。表面活性剂的直链长度对KR2菌株生长的影响为：直链越短，对分枝杆菌KR2菌株生长的抑制作用越大。