Flow cytometric measurement of the clearance rate in the blue mussel Mytilus edulis and the development of a new individual exposure system for aquatic immunotoxicological studies

Animals in poor health condition are not relevant biological models. The current study focused on the use of the clearance rate of Mytilus edulis to assess the gross physiological condition of individuals maintained in stressful experimental conditions. This approach was developed in a new, highly controlled experimental exposure device designed to investigate individual responses in aquatic ecotoxicological studies. Both clearance rate values and immune parameters analysis indicated that the health condition of mussels kept in 50ml tubes for 24h or 48h was not altered compared to controls, while most parameters were depressed after 72h. Moreover, this study confirms the relevance of flow cytometric for the measurement of clearance rate compared to techniques utilizing microscopy. Current results prompted us to perform further 24h chemical exposure using this "in tubo" device.     

Organohalogen pollutants in herring from the northern Baltic Sea: concentrations, congener profiles and explanatory factors

Organohalogen contaminants were investigated in Baltic herring caught from three catchment areas in the Baltic Sea, off the coasts of Finland. Pools of both small and large herring were analysed for polychlorinated biphenyls (PCB), dibenzo-p-dioxins, dibenzofurans, naphthalenes, camphenes (toxaphene), polybrominated diphenyl ethers and the pesticide DDT and its metabolites. PCB concentrations per fresh weight in small herring were at the same level in all catchment areas, i.e. the Bothnian Bay, the Bothnian Sea and the Gulf of Finland, revealing no hot spots and reflecting most likely long term emissions and atmospheric deposition. Differences in the levels and/or congener profiles of other contaminants between catchment areas may be explained by point sources. Similar concentrations in small and large herring in the Gulf of Finland were possibly due to their common nutrition. In the other areas, differences between small and large herring most likely reflected their different food sources.     

Local to regional emission sources affecting mercury fluxes to New York lakes

Lake-sediment records across the Northern Hemisphere show increases in atmospheric deposition of anthropogenic mercury (Hg) over the last 150 years. Most of the previous studies have examined remote lakes affected by the global atmospheric Hg reservoir. In this study, we present Hg flux records from lakes in an urban/suburban setting of central New York affected also by local and regional emissions. Sediment cores were collected from the Otisco and Skaneateles lakes from the Finger Lakes region, Cross Lake, a hypereutrophic lake on the Seneca River, and Glacial Lake, a small seepage lake with a watershed that corresponds with the lake area. Sediment accumulation rates and dates were established by ²¹⁰Pb. The pre-anthropogenic regional atmospheric Hg flux was estimated to be 3.0 μg m⁻² yr⁻¹ from Glacial Lake, which receives exclusively direct atmospheric deposition. Mercury fluxes peaked during 1971–2001, and were 3 to more than 30 times greater than pre-industrial deposition. Land use change and urbanization in the Otisco and Cross watersheds during the last century likely enhanced sediment loads and Hg fluxes to the lakes. Skaneateles and Glacial lakes have low sediment accumulation rates, and thus are excellent indicators for atmospheric Hg deposition. In these lakes, we found strong correlations with emission records for the Great Lakes region that markedly increased in the early 1900s, and peaked during WWII and in the early 1970s. Declines in modern Hg fluxes are generally evident in the core records. However, the decrease in sediment Hg flux at Glacial Lake was interrupted and has increased since the early 1990s probably due to the operation of new local emission sources. Assuming the global Hg reservoir tripled since the pre-industrial period, the contribution of local and regional emission sources to central New York lakes was estimated to about 80% of the total atmospheric Hg deposition.     

Symptomatic profile and health‐related quality of life of persons affected by the Prestige catastrophe

This study analyses the impact of the Prestige oil spill on the mental health and the perception of physical health and functional capacity in the affected population. The sample comprised 926 residents from the section of the Spanish coast affected by the oil spill. The data was collected slightly more than one year after the accident. Scales referring to clinical symptoms (SCL‐36) and health‐related quality of life (SF‐36) were administered. The results suggest that individuals with higher degrees of exposure or residing in areas closest to the spill show lower levels of mental health in comparison to those with lower levels of exposure or living in areas farther away from the spill. This study also finds that women and fishermen tend to suffer more from the consequences of these types of disaster.     

医护人员血源性病原体职业危害研究进展

本研究的目的是了解医护人员血源性病原体职业接触流行特征、职业危害及其研究进展。结果表明,发生针刺及其他锐器损伤的最主要的职业群体是护理人员,最容易导致医护人员损伤的锐器为注射器针头、玻璃安瓿、缝合针和手术刀片等,导致医护人员锐器伤的最常见的医疗操作是注射、缝合和采血。发生血源性病原体职业接触后,医护人员可能感染HBV、HCV及HIV等多种血源性病原体。国内既往研究大多关注伤害发生率及相关危险因素,未对血源性病原体职业接触后感染HBV、HCV及HIV的风险开展研究,尚需通过建立队列,开展前瞻性调查研究。     

Occupational risk from static magnetic fields of MRI scanners

The health care staff operating magnetic resonance imaging (MRI) scanners are exposed to static magnetic field of significant spatial heterogenity and high level of flux density—usually existing permanently during the shift. The personnel can also be exposed to pulses of magnetic field of high rate of rise/fall, so-called gradient fields, which exist only during examination of patients. The level of workers’ exposure depends both on the type of the magnet and on the ergonomical characteristic of design of the particular MRI scanner. This paper presents the current state of the art on occupational exposure to static magnetic field health effects, gaps in scientific data, MRI workers’ exposure characteristics, research needs, and suggestions for the exposure assessment protocol for future investigations.     

Practical aspects of occupational EMF exposure assessment

Electromagnetic fields exposure assessment methodology is briefly presented. The basic problems defined for the practical use of electromagnetic fields measurements and numerical calculations carried out for workers exposure assessment in real occupational situations are discussed. The examples of data from real workplace are presented, focusing: spatial distribution of electromagnetic fields affecting worker’s body, complex characteristics of the frequency content, workers activities/moving in the workplace, field impedance, etc. The situation when the use of calculations is required is discussed. The basic requirements for workers exposure assessment protocols are presented. The possible range of the use of internal and external measures of exposure level is also discussed.     

Stress-70 as indicator of heavy metals accumulation in blue mussel Mytilus edulis

The induction of stress-70 (Hsp70) in the blue mussels Mytilus edulis from southern Baltic after exposure to cadmium, lead and copper, administrated individually and/or as a mixture, was investigated by means of standard Western blotting using a clone 3A3 monoclonal antibody. The general course of the stress-70 induction was found to be similar for all tested metals. It could be characterised by three main features: (1) The induction of stress-70 proteins was only slightly increased at low metal concentration. (2) Strong induction of stress-70 occurred at high concentration of metals. Combination of metals (Cd+Cu) was found to increase the stress-70 level more intensely than related concentrations of singularly applied metals. (3) Five weeks of long depuration had no effect on stress-70 levels. Experiments revealed different induction of stress-70 as a response to cadmium, lead and copper exposure and confirm that the applied technique might serve as a versatile tool to assess invertebrate stress reaction to environmentally relevant heavy metals.     

Concentrations,Cumulative Exposure and Critical Levels of Ozone in Ireland

Concentrations of tropospheric ozone(O₃) and exceedance of critical levels to vegetationhave been investigated and mapped for Ireland. Hourlyozone concentration data (1995–1997) at 7 sevenmonitoring stations and the CORINE landcover database,supported by a Geographical Information System, wereused. AOT40 (Accumulated exposure Over a Threshold of 40ppb) was calculated for daylight hours for each station,and mapped using surface interpolation. Average O₃concentrations vary from year to year, and were estimatedto be 28 ppb, 26 ppb and 24 ppb for 1995, 1996 and 1997respectively. Ozone concentrations show a large diurnalvariation, with a maximum in the afternoon and a minimumat night-time. The critical level for crops and (semi-)natural vegetation was exceeded in all years examined.The highest exceedance occurred in 1995, where thecritical level was exceeded for almost 35% of the mappedarea. Approximately 15% and 1% of the mapped area wasexposed to exceedance levels during 1996 and 1997respectively. The maximum cumulative exposures (AOT40)were approximately 5000, 3890 and 3230 ppbh in 1995, 1996and 1997 respectively. The critical level for forests wasnot exceeded during the period of investigation.     

Personal carbon monoxide exposures of preschool children in Helsinki, Finland—comparison to ambient air concentrations

The associations of personal carbon monoxide (CO) exposures with ambient air CO concentrations measured at fixed monitoring sites, were studied among 194 children aged 3–6 yr in four downtown and four suburban day-care centers in Helsinki, Finland. Each child carried a personal CO exposure monitor between 1 and 4 times for a time period of between 20 and 24 h. CO concentrations at two fixed monitoring sites were measured simultaneously. The CO concentrations measured at the fixed monitoring sites were usually lower (mean maximum 8-h concentration: 0.9 and 2.6 mg m⁻³) than the personal CO exposure concentrations (mean maximum 8-h concentration: 3.3 mg m⁻³). The fixed site CO concentrations were poor predictors of the personal CO exposure concentrations. However, the correlations between the personal CO exposure and the fixed monitoring site CO concentrations increased (−0.03–−0.12 to 0.13–0.16) with increasing averaging times from 1 to 8 h. Also, the fixed monitoring site CO concentrations explained the mean daily or weekly personal CO exposures of a group of simultaneously measured children better than individual exposure CO concentrations. This study suggests that the short-term CO personal exposure of children cannot be meaningfully assessed using fixed monitoring sites.