Pregnancy after preimplantation genetic diagnosis for Sanjad–Sakati syndrome

Sanjad–Sakati syndrome (SSS) is an autosomal recessive disorder characterized by congenital hypoparathyroidism, growth and mental retardation. In Saudi Arabia, the disease is caused by a deletion of 12 bp (155-166nt) in the tubulin-specific chaperone E gene. In a family with two affected siblings with SSS, preimplantation genetic diagnosis (PGD) was performed. Fluorescent PCR (F-PCR) was utilized to check the heterozygosity and the homozygosity status of the parents and the affected children, respectively. F-PCR was then optimized for single-cell analysis by using peripheral blood lymphocytes. The patient underwent a cycle with intra-cytoplasmic sperm injection. A total of 11 embryos were obtained and biopsied. There were five heterozygous, three homozygous affected and three normal embryos. One heterozygous and one normal embryo were transferred because of their very good quality (morula). A singleton pregnancy was obtained, and amniosynthesis confirmed the presence of the heterozygous fetus. These results show for the first time, the feasibility of PGD for SSS. Copyright © 2004 John Wiley & Sons, Ltd.     

Hot extrusion process modeling using a coupled upper bound-finite element method

A thermo-mechanical model has been developed for modeling of hot extrusion processes. Accordingly, an admissible velocity field was first proposed by means of stream function method and then, extrusion pressure as well as temperature variations within the metal and the die were predicted employing a combined upper bound and Petrov–Galerkin finite element analysis. In order to evaluate the model predictions, hot extrusion of AA6061-10%SiC_p was considered under both isothermal and non-isothermal conditions and the predicted force–displacement diagrams under various extrusion conditions were compared with the experimental ones and reasonable consistency was found between the two sets of results.     

Problems and issues in laser beam welding of aluminum–lithium alloys

Aluminum–lithium (Al–Li) alloys are very attractive for potential aerospace applications due to their lower density, higher specific strength and rigidity, better corrosion and fatigue crack growth resistance properties, compared to conventional aluminum alloys. Laser beam welding (LBW), being an advanced joining method, has already been approved for applications involving aluminum alloys. However, there are still a number of problems and issues to be answered and solved in LBW of Al–Li alloys. In this review, the properties of Al–Li alloys and the characteristics of LBW are introduced, the formation and prevention of the main weld defects such as porosity and hot cracking are discussed, and then the weld microstructure and the joint mechanical properties are described in highlight. At the end, an outlook on future trends is presented.     

Rethinking the study of human–wildlife coexistence

Although coexistence with wildlife is a key goal of conservation, little is known about it or how to study it. By coexistence we mean a sustainable though dynamic state in which humans and wildlife coadapt to sharing landscapes, where human interactions with wildlife are effectively governed to ensure wildlife populations persist in socially legitimate ways that ensure tolerable risk levels. Problems that arise from current conflict-oriented framing of human–wildlife interactions include reinforcing a human–nature dichotomy as fundamentally oppositional, suggesting coexistence requires the absence of conflict, and skewing research and management toward direct negative impacts over indirect impacts and positive aspects of living with wildlife. Human behavior toward wildlife is framed as rational calculus of costs and benefits, sidelining emotional and cultural dimensions of these interactions. Coexistence is less studied due to unfamiliarity with relevant methodologies, including qualitative methods, self-reflexivity and ethical rigor, and constraints on funding and time. These challenges are illustrated with examples from fieldwork in India and Africa. We recommend a basic approach to case studies aimed at expanding the scope of inquiries into human–wildlife relations beyond studies of rational behavior and quantification of costs and benefits of wildlife to humans.     

A mixed methodology for evaluating use of evidence in conservation planning

Conservation practitioners widely recognize the importance of making decisions based on the best available evidence. However, the effectiveness of evidence use in conservation planning is rarely assessed, which limits opportunities to improve evidence-based practice. We devised a mixed methodology for empirically evaluating use of evidence that applies social science tools to systematically appraise what kinds of evidence are used in conservation planning, to what effect, and under what limitations. We applied our approach in a case study of the Nature Conservancy of Canada (NCC), a leading land conservation organization. We conducted qualitative and quantitative analyses of 65 NCC planning documents (n = 13 in-depth) to identify patterns in evidence use, and surveyed 35 conservation planners to examine experiences of and barriers to using evidence. Although claims in plans contained a wide range of evidence types, 26% of claims were not referenced or associated with an identifiable source. Lack of evidence use was particularly apparent in claims associated with direct threats, particularly those identified as low (71% coded as insufficient or lacking evidence) or medium (45%) threats. Survey participants described relying heavily on practitioner experience and highlighted capacity limitations and disciplinary gaps in expertise among planning teams as barriers to using evidence effectively. We found that although time-intensive, this approach yielded actionable recommendations for improving evidence use in NCC conservation plans. Similar mixed-method assessments may streamline the process by including interviews and refining the document analysis frames to target issues or sections of concern. We suggest our method provides an accessible and robust point of departure for conservation practitioners to evaluate whether the use of conservation planning reflects in-house standards and more broadly recognized best practices.     

The wild ancestors of domestic animals as a neglected and threatened component of biodiversity

Domestic animals have immense economic, cultural, and practical value and have played pivotal roles in the development of human civilization. Many domesticates have, among their wild relatives, undomesticated forms representative of their ancestors. Resurgent interest in these ancestral forms has highlighted the unclear genetic status of many, and some are threatened with extinction by hybridization with domestic conspecifics. We considered the contemporary status of these ancestral forms relative to their scientific, practical, and ecological importance; the varied impacts of wild–domestic hybridization; and the challenges and potential resolutions involved in conservation efforts. Identifying and conserving ancestral forms, particularly with respect to disentangling patterns of gene flow from domesticates, is complex because of the lack of available genomic and phenotypic baselines. Comparative behavioral, ecological, and genetic studies of ancestral-type, feral, and domestic animals should be prioritized to establish the contemporary status of the former. Such baseline information will be fundamental in ensuring successful conservation efforts.     

Mitigation translocation as a management tool

Mitigation translocation is a subgroup of conservation translocation, categorized by a crisis-responsive time frame and the immediate goal of relocating individuals threatened with death. However, the relative successes of conservation translocations with longer time frames and broader metapopulation- and ecosystem-level considerations have been used to justify the continued implementation of mitigation translocations without adequate post hoc monitoring to confirm their effectiveness as a conservation tool. Mitigation translocations now outnumber other conservation translocations, and understanding the effectiveness of mitigation translocations is critical given limited global conservation funding especially if the mitigation translocations undermine biodiversity conservation by failing to save individuals. We assessed the effectiveness of mitigation translocations by conducting a quantitative review of the global literature. A total of 59 mitigation translocations were reviewed for their adherence to the adaptive scientific approach expected of other conservation translocations and for the testing of management options to continue improving techniques for the future. We found that mitigation translocations have not achieved their potential as an effective applied science. Most translocations focused predominantly on population establishment- and persistence-level questions, as is often seen in translocations more broadly, and less on metapopulation and ecosystem outcomes. Questions regarding the long-term impacts to the recipient ecosystem (12% of articles) and the carrying capacity of translocation sites (24% of articles) were addressed least often, despite these factors being more likely to influence ultimate success. Less than half (47%) of studies included comparison of different management techniques to facilitate practitioners selecting the most effective management actions for the future. To align mitigation translocations with the relative success of other conservation translocations, it is critical that future mitigation translocations conform to an established experimental approach to improve their effectiveness. Effective mitigation translocations will require significantly greater investment of time, expertise, and resources in the future.     

Socioeconomic development and ecological traits as predictors of human–bird conflicts

Because of the significant impacts on both human interests and bird conservation, it is imperative to identify patterns and anticipate drivers of human–bird conflicts (HBCs) worldwide. Through a global systematic review, following the PRISMA 2020 guidelines, we analyzed the socioeconomic factors and bird ecological traits driving the degree of knowledge and extent of HBCs. We included 166 articles published from 1971 to 2020 in our analyses through which we built a profile of the socioeconomic conditions of 52 countries with reported conflicts and the ecological traits of the 161 bird species involved in HBCs. Although HBC expanded worldwide, it had the greatest impact in less-developed countries (estimate 0. 66 [SE 0.13], p< 0.05), where agriculture is critical for rural livelihoods. Species with a relatively greater conflict extent had a relatively broader diet (estimate 0.80 [SE 0.22], p<0.05) and an increasing population trend (estimate 0.58 [SE 0.15], p<0.05) and affected human interests, such as agriculture and livestock raising. In countries with greater biodiversity, HBCs caused greater socioeconomic impacts than in more developed countries. Our results highlight the importance of understanding and addressing HBCs from multiple perspectives (ecological, sociocultural, and political) to effectively protect both biodiversity and local livelihoods.     

Rainfall conditions and rainwater harvesting potential in the urban area of Khartoum

Runoff water management is among the inherent challenges which face the sustainability of the development of arid urban centers. These areas are particularly at risk from flooding due to rainfall concentration in few heavy showers. On the other hand, they are susceptible to drought. The capital of Sudan (Khartoum) stands as exemplary for these issues. Hence, this research study aims at investigating the potential of applying rainwater harvesting (RWH) in Khartoum City Center as a potential urban runoff management tool. Rapid urbanization coupled with the extension of impervious surfaces has intensified the heat island in Khartoum. Consequently, increased frequency of heat waves and dust storms during the dry summer and streets flooding during the rainy season have led to environmental, economical, and health problems. The study starts with exposing the rainfall behavior in Khartoum by investigating rainfall variability, number of raindays, distribution of rain over the season, probability of daily rainfall, maximum daily rainfall and deficit/surplus of rain through time. The daily rainfall data show that very strong falls of >30 mm occur almost once every wet season. Decreased intra- and inter-annual rainfall surpluses as well as increased rainfall concentration in the month of August have been taking place. The 30-year rainfall variability is calculated at decade interval since 1941. Increasing variability is revealed with 1981–2010 having coefficients of variation of 66.6% for the annual values and 108.8–118.0% for the wettest months (July–September). Under the aforementioned rainfall conditions, this paper then explores the potential of RWH in Khartoum City Center as an option for storm water management since the drainage system covers only 40% of the study area. The potential runoff from the 6.5 km² center area is computed using the United States Natural Resources Conservation Services method (US-NRCS), where a weighted Curve Number (CN) of 94% is found, confirming dominant imperviousness. Rainfall threshold for runoff generation is found to be 3.3 mm. A 24,000 m³ runoff generated from a 13.1 mm rainfall (with 80% probability and one year return period) equals the drainage system capacity. An extreme rainfall of 30 mm produces a runoff equivalent to fourfold the drainage capacity. It is suggested that the former and latter volumes mentioned above could be harvested by applying the rational method from 18% and 80% rooftops of the commercial and business district area, respectively. Based on the above results, six potential sites can be chosen for RWH with a total roof catchment area of 39,558 m² and potential rooftop RWH per unit area of 0.033 m³. These results reflect the RWH potential for effective urban runoff management and better water resources utilization. RWH would provide an alternative source of water to tackle the drought phenomenon.