The use of the embryo‐culture techniques in the research on the Teratogenic and Mutagenic properties of metals†

Actually, embryos can be cultured from the one‐cell stage up to the blastocyst stage, and their development can be easily monitored at any time: severe effects caused by toxic compounds are traduced by rapid embryonic death, less pronounced effects can be expressed by a lowered cleavage activity or by an arrest of the development from a particular stage. The system can be improved by transferring the embryos at the blastocyst stage in another more complete medium where they can “implant”; and form an inner cell mass with differentiated ectoderm and endoderm. Since last years, it has also become possible to culture postimplantation rodent embryos for short periods involving a number of particularly critical stages of organogenesis, such as the formation and closure of the anterior neuropore. Embryo‐culture also represents a useful system to study cytogenetic effects of chemicals which are often linked to lethal or teratogenic effects. These different possibilities are illustrated by examples of studies already performed with metals, and dealing with their teratogenic and/or cytogenetic effects on pre‐ and postimplantation rodents.     

Genetic toxicology of metal compounds: An examination of appropriate cellular models †

Bacterial systems have not had success predicting metal carcinogenicity. Hypotheses explaining this failure are examined. Using a broad genetic endpoint, λ prophage induction, under sub‐toxic growing conditions, genotoxicity is seen for compounds of chromium, manganese, lead, molybdenum and tungsten. Copper, manganese, arsenic and molybdenum compounds enhanced UV mutagenesis in E. coli WP2.

The toxicity of metal compounds to cultured mammalian cells correlates well with rat oral LD₅₀ values. Whereas insolubility can present problems in bacterial studies, concentrations of metal compounds toxic to mammalian cells can be determined even in the presence of precipitate, and sometimes [Pb(NO₃)₂, BaCl₂ and BeCl₂] occurs only in its presence. PbS and MnS, which are insoluble, are much more toxic than the more soluble compounds Pb(NO₃)₂ and MnCl₂. These results demonstrate the importance of cellular phagocytosis of insoluble metal compounds as a factor in studying the toxicity and genotoxicity of metal compounds.     

Oxidative and genotoxic effects of bisphenol A on primary gill cell culture of Lake Van Fish (Alburnus tarichi Güldenstädt, 1814)

Lake Van is the largest lake in Turkey. The lake limits lifespan due to its high pH and brackish water. For this reason, only a single species of fish (Van Fish) is living in the lake that has been adapted to these conditions. In the present study, we investigated the total oxidant status (TOS), total antioxidant status (TAS), malondialdehyde (MDA) level and DNA damage effect of bisphenol A (BPA) (10^?7, 10^?6 and 10^?5?M) on primary gill cell culture of Van Fish for 24 and 48?h of incubation periods. TAS levels were not changed when compared to those of the control group, but TOS levels were decreased in both 24 and 48?h. The MDA level increased only at the highest concentration (10^?5) at the end of 12 and 24?h (p?.05). DNA damage increased only at the 10^?5?M concentration after 48?h. At the end of the experiment, BPA exposure caused lipid peroxidation and genotoxic effect. These results indicate that high levels of BPA exposure induced oxidative stress and DNA damage by time- and concentration-dependent fashion in the gill cell culture of Van Fish. Gill cell culture is a useful model for the rapid identification of the harmful effects of chemicals in the aquatic environment.     

The role of Confucianism in South Korea's emergency management system

Numerous Western researchers have examined the influence of Confucianism on development in Asia, but almost no South Korean researchers have studied the topic in regard to emergency management in their own country. This study begins by considering Confucianism as a social culture. Next, it goes on to evaluate its role in South Korea's emergency management system, contributing, ultimately, to efficiencies in emergency management. Drawing on a literature review and a case study, the paper assesses the double‐ and single‐faced approaches, using four major Confucian components: destiny; family; ritual; and relation. The double‐faced approach includes the positive and negative aspects of emergency management, whereas the single‐faced approach incorporates only its positive aspects. This paper provides, for the first time, a systematic analysis of the relationship between Confucianism and emergency management in South Korea. Its key finding is that the double‐faced approach needs to be transformed into a single‐faced approach through active facilitation of behavioural change.     

高校安全文化建设现状及对策研究——以L大学为例

为了改善L大学安全状况,提高L大学安全文化建设水平,采用文献对比分析法研究高校安全文化定义及其理论实质,从狭义的角度提出高校安全文化是高校管理的一系列安全理念,具体化为32条高校安全文化核心元素。以L大学为研究样本,定性分析其安全文化建设内容;编制高校安全文化定量测量量表,对其安全文化建设水平进行定量测量。评价结果表明L大学安全文化建设存在缺欠,提出了实用性对策措施和建议。     

论政府安全文化的若干基本问题

为明晰政府安全文化的基本理论问题,进而确立政府安全文化研究与实践的基本理论框架,根据组织安全文化与政府文化的定义,提出政府安全文化的定义,并依次从政府安全文化的主体、载体、主要内容与直接目的等9方面出发,详细探讨政府安全文化的重要内涵。在此基础上,深入解析政府安全文化的建设思路与路径。结果表明:政府安全文化建设是推进政府安全监督管理体制机制改革和提升政府安全监督管理水平的一条重要思路和措施,政府安全文化的建设思路与路径主要包括4方面内容,即建设内容、建设方向、核心理念与建设途径。     

The determinants of employee participation in occupational health and safety management

This article focuses on employee direct participation in occupational health and safety (OHS) management. The article explains what determines employee opportunities to participate in OHS management. The explanatory framework focuses on safety culture and safety management at workplaces. The framework is empirically tested using Estonian cross-sectional, multilevel data of organizations and their employees. The analysis indicates that differences in employee participation in OHS management in the Estonian case could be explained by differences in OHS management practices rather than differences in safety culture. This indicates that throughout the institutional change and shift to the European model of employment relations system, change in management practices has preceded changes in safety culture which according to theoretical argument is supposed to follow culture change.     

高速铁路信号系统的安全管理评价研究

高速铁路信号系统要通过安全管理来保障其开发与运行中的安全相关活动符合系统安全计划的要求。为了评估安全管理活动的可信性,提出基于系统安全分析技术的安全管理评价方法。通过建立安全管理流程与系统安全功能相关联的概念模型,使用安全文化危险与可操作性研究(SCHAZOP)辨识出安全管理流程中的行为偏差,基于失效传导转换符号(FPTN)建立管理角色的安全文化失效模型,最终将管理行为失效模式转换成组件故障树作为安全管理评价证据。研究结果表明,安全管理行为偏差体现了信号系统开发与运营过程中的安全文化特征;辨识与分析安全文化失效,为信号系统安全管理活动的可信性提供了评价依据。     

Blurred Borderlands: Sustainability and the Urban/Nature Divide at the Frick Environmental Center

ABSTRACT

Sustainable development is rhetorically framed as exhibiting natural processes, reflected both symbolically and materially. Taking the Frick Environmental Center of Pittsburgh, Pennsylvania as a rhetorical artifact, I argue that spaces where sustainable human-made structures and human-cultivated landscapes meet, reflect blurred borderlands of a culturally constructed urban/nature divide. Considering the Frick Environmental Center as an experiential landscape, my mixed methodology of textual analysis and rhetorical field work identifies how naturalized framing of sustainability bridges the urban/nature divide, inviting restoration, preservation, and construction efforts that are more environmentally engaged.     

应急准备文化的推进与实践

应急准备文化是应急管理工作中的重要组成部分,应急准备文化包括知识文化、应急意识和观念文化以及行为文化。应急文化最终体现在应急行动上。推进和实践应急准备文化应从理念和意识的形成,抗灾救灾理论研究和应急文化的教育,必要的组织和制度保障,人才队伍建设和培养,抗逆力的培养和形成,政府、社会和公民责任和义务,公共危机教育和认真总结分析巨灾后的应急救援工作得失等多方面进行。