An investigation of methods for enriching trophoblast from maternal blood

Trophoblast deportation is known to occur in normal human pregnancy, but it is not yet clear whether these cells routinely enter the maternal peripheral circulation and are available as a source of fetal DNA for non-invasive prenatal diagnosis of genetic disorders. To resolve this issue requires an efficient method of enriching trophoblast from maternal blood combined with a means to confirm its identity. Five different techniques were tested on ten retroplacental blood samples to determine the most sensitive and operator-efficient method. Lysis of red cells alone gave the best recovery of trophoblast but had to be discounted, together with Ficoll density gradient centrifugation, due to the very low purity and the excessive time required. Fluorescence-activated cell sorting (FACS) of pre-enriched trophoblast resulted in the lowest recovery rate (8 per cent) despite a 3250-fold enrichment and a very high purity. Immunomagnetic beads (Dynabeads) coated with anti-CD 16 antibody proved to be the best method for the subsequent immunocytochemical characterization of deported trophoblast. However, IO beads coated with anti-CD45 antibody may be more useful for isolating trophoblast for prenatal diagnosis due to the high purity, enrichment (32-fold), and recovery rate (78 per cent) obtained with this method.     

Prenatal exclusion of stickler syndrome

Stickler syndrome is an autosomal dominant disorder of the connective tissue which includes ocular and systemic manifestations. We report on a large kindred in which we were able to demonstrate very tight linkage between the disease and the type II collagen gene (COL2A1) (LOD score 3·91 at θ=0). In a family in which the father and one of his daughters were severely affected, DNA analysis from a chorionic villus sample demonstrated that the fetus possessed the normal allele of COL2A1. Thereafter a normal child was born.     

中扬子台地北缘灯影组碳酸盐岩成岩作用序列及成岩模式

研究区主要的成岩作用类型有胶结、溶解、硅化、白云石化、压实-压溶和去白云石化作用等，详细研究了每种成岩作用的矿物岩石学特征及对孔隙形成、演化的影响。把研究区成岩环境划分为大气淡水成岩环境、海水成岩环境、混合水成岩环境、埋藏成岩环境和晚期表生成岩环境五大类．总结了每种成岩环境的成岩组构特征、成岩标志、δ18O和δC及微量元素Na+、Sr2+的分布规律。此外，还探讨了深水斜坡相粉-细晶白云岩和台地浅滩相颗粒白云岩的成岩演化序列与孔隙演化，最后概括出了研究区碳酸盐岩的成岩作用模式。     

云南安宁工业区工业经济-水资源-水污染系统仿真模型及策略分析

水资源短缺和水污染是安宁工业区经济发展的重要限制因素。本文将该工业区分解为3个子区,应用系统动态学方法,建立多区相关的工业经济-水资源-水污染系统仿真模型,分别对各子区1985至2000年的工业产值、工业用水差距和水质进行多方案预测。在子区域综合的基础上,结合多级决策树法,筛选出安宁工业区协调工业经济、水资源和水质之间关系的最佳方案。     

VOC removal from contaminated groundwater through membrane pervaporation. (Ⅱ): 1,1,1-trichloroethane-SDS surfactant solution system

IntroductionTheconventionalmethodforsubsurfaceremediationofgroundwatercontaminationwithVOCsistheso called“pump and treat”technology .Thelimitationofthisapproachistheprohibitivelylongtreatmenttimeforremediatingthegroundwatercontaminatedwithcommonchlorinatedsolventsthathavedensitiesgreaterthanthatofwater(classifiedasDNAPLs densenon aqueousphaseliquids) .Thisislargelyduetotheverylowwatersolubilityandlowbiodegradabilityofthisclassofsolventsandthetendencyofthesolventssettlinginthebottomofanaqu…     

Quantitative structure-property relationship of aromatic sulfur-containing carboxylates

IntroductionItiswidelyrecognizedthatthephysicochemicalprofileoforganicchemicalslargelydeterminestheirdistributionbetweenenvironmentalmedia .Fortheenvironmentalbehaviorsoforganiccontaminants,watersolubility(SW)andoctanol waterpartitioncoefficient(KOW)aretremendouslyimportant.Octanol waterpartitioncoefficienthasbeenwidelyrelatedtobiochemicaland orbiologicalactivityinquantitativestructure activityrelationships(QSARs) (Leo ,1 971 ) .Watersolubilitycorrespondstothedispersiontendencyandtothereca…     

粤西震旦系含金建造及与金矿的关系

粤西震旦系Z~b组和Z~c组是重要的含金建造。原岩为一套陆源碎屑岩,其中夹有少量中酸性凝灰岩。建造中的金丰度高于克拉克值2-4倍,金的丰度及其分布主要受变质岩的基性程度,硫化物及有机质数量控制。     

毛细管气相色谱法分析1,3—丁二烯中炔烃杂质含量

介绍了一种毛细管毛相谱法测定１，３－丁烯中炔烃质含量的新试验方法，该方法以氮气为载气，采用氢火焰离子化检测器，样品经分离后，采用外标法定量，操作简便，准确。该方法还可用于１，３－丁二烯纯度分析 。     

云南省经济增长与能源消耗的计量模型研究

根据云南省1985~2003年能源消耗的统计数据以及人均GDP统计数据,分析人均GDP与能源消耗之间的相关关系,选取相关性较好的能耗指标(煤炭消耗量、石油消耗量、天然气消耗量、电力消耗量)建立两者之间的计量模型。并在此基础上分析能源消耗与人均GDP增长的关系。经研究发现,云南省经济增长与能源消耗呈类似于EKC的倒U型曲线,且曲线尚未到达转折点,能源消耗量随着经济增长将持续增长。有必要采取有利措施倡导“循环经济”、“绿色GDP”等使曲线走势向良性方向发展。