A signal-amplified whole-cell biosensor for sensitive detection of Hg2+ based on Hg2+-enhanced reporter module

A signal-amplified mercury sensing biosensor with desired sensitivity was developed through firstly using the GFP mutant with fluorescence increasing response towards Hg²⁺ as the reporter module. The developed biosensor showed response for Hg²⁺ in a relatively wide range of 1–10,000 nmol/L, and the detection limit was improved one or two orders of magnitude in comparison with most metal-sensing biosensors in similar constructs. In addition, the biosensor could distinguish Hg²⁺ easily from multiple metal ions and displayed strong adaptability to extensive pH conditions (pH 4.0–10.0). More importantly, the developed biosensor was able to provide an initial assessment of Hg²⁺ spiked in the environmental water with the recoveries between 85.70% and 112.50%. The signal-amplified strategy performed by the modified reporter module will be widely applicable to many other whole-cell biosensors, meeting the practical requirements with sufficient sensing performance.     

Life cycle assessment of spray dried soluble coffee and comparison with alternatives (drip filter and capsule espresso)

This paper aims to evaluate the environmental burdens associated with spray dried soluble coffee over its entire life cycle and compare it with drip filter coffee and capsule espresso coffee. It particularly aims to identify critical environmental issues and responsibilities along the whole life cycle chain of spray dried coffee. This life cycle assessment (LCA) specifically uses foreground data obtained directly from coffee manufacturers and suppliers. Aside from energy consumption and greenhouse gases emissions, water footprint is also studied in detail, including regionalization of water impacts based on the ecological scarcity method 2006. Other impact categories are screened using the IMPACT 2002+ impact assessment method.The overall LCA results for a 1 dl cup of spray dried soluble coffee amounts approximately to 1 MJ of primary non-renewable energy consumption, to emissions of 0.07 kg of CO_2-eq, and between 3 and 10 l of non-turbined water use, depending on whether or not the coffee cultivation is irrigated and wet treated. When considering turbined water, use can be up to 400 l of water per cup. Pouch – and to a lesser extent metal can packaging alternatives – show lower environmental burdens than glass or sticks.On average, about one half of the environmental footprint occurs at a life cycle stage under the control of the coffee producer or its suppliers (i.e., during cultivation, treatment, processing, packaging up to distribution, along with advertising) and the other half at a stage controlled by the user (shopping, appliances manufacturing, use and waste disposal). Key environmental parameters of spray dried soluble coffee are the amount of extra water boiled and the efficiency of cup cleaning during use phase, whether the coffee is irrigated or not, as well as the type and amount of fertilizer used in the coffee field. The packaging contributes to 10% of the overall life cycle impacts.Compared to other coffee alternatives, spray dried soluble coffee uses less energy and has a lower environmental footprint than capsule espresso coffee or drip filter coffee, the latter having the highest environmental impacts on a per cup basis. This study shows that a broad LCA approach is needed to help industry to minimize the environmental burdens directly related to their products. Including all processes of the entire system is necessary i) to get a comprehensive environmental footprint of the product system with respect to sustainable production and consumption, ii) to share stakeholders responsibility along the entire product life cycle, and iii) to avoid problem shifting between different life cycle stages.     

水环境监测质量控制相关措施分析

本文首先从水环境监测质量控制的内涵出发,简要概述了当下发展中存在的主要问题,提出质量控制的相关措施,希望能够为从业人员提供参考。     

Technologies for pollutant removal and resource recovery from blackwater: a review

● Blackwater is the main source of organics and nutrients in domestic wastewater. ● Various treatment methods can be applied for resource recovery from blackwater. ● Blackwater treatment systems of high integration and efficiency are the future trend. ● More research is needed for the practical use of blackwater treatment systems. Blackwater (BW), consisting of feces, urine, flushing water and toilet paper, makes up an important portion of domestic wastewater. The improper disposal of BW may lead to environmental pollution and disease transmission, threatening the sustainable development of the world. Rich in nutrients and organic matter, BW could be treated for resource recovery and reuse through various approaches. Aimed at providing guidance for the future development of BW treatment and resource recovery, this paper presented a literature review of BWs produced in different countries and types of toilets, including their physiochemical characteristics, and current treatment and resource recovery strategies. The degradation and utilization of carbon (C), nitrogen (N) and phosphorus (P) within BW are underlined. The performance of different systems was classified and summarized. Among all the treating systems, biological and ecological systems have been long and widely applied for BW treatment, showing their universality and operability in nutrients and energy recovery, but they are either slow or ineffective in removal of some refractory pollutants. Novel processes, especially advanced oxidation processes (AOPs), are becoming increasingly extensively studied in BW treatment because of their high efficiency, especially for the removal of micropollutants and pathogens. This review could serve as an instructive guidance for the design and optimization of BW treatment technologies, aiming to help in the fulfilment of sustainable human excreta management.     

Effect of audits on the extent of compliance with wastewater discharge limits

This study explores the effect of environmental self-audits (“audits”), which represent an important type of environmental management system practice, on the extent of facilities’ compliance with wastewater discharge limits. Theoretically, audits may (1) improve compliance by enhancing the effectiveness of treatment technologies and pollution prevention methods, (2) undermine compliance by distracting facilities’ personnel with audit-related administrative burdens, or (3) not influence compliance because these effects neutralize each other. By examining the extent of compliance, our study’s results reflect both improvement toward and beyond compliance. By assessing compliance with multiple pollutants separately, our study examines whether audits influence the control of different pollutants uniformly. Lastly, we employ a dynamic panel estimator, which allows us to explore whether facilities adjust their discharges dynamically, while controlling for any inertia in facilities’ pollution control systems. Our study empirically examines the U.S. chemical manufacturing sector between 1999 and 2001 using survey and publicly available EPA data.     

Assessing local discourses on water in a South African newspaper

Academic understandings of environmental discourses are primarily based on global and national accounts, despite widespread acceptance of the local as an essential site of environmental action. Local water issues have been studied in a number of ways, including interviews and ethnographies that show the impacts of water scarcity, examine the role of mediating technologies, and provide diverse perspectives on governance. An overarching impression of key narratives and concerns at local scales, however, is lacking. In this paper, we examine water coverage in The Sowetan, a South African newspaper known for its distinctive voice, as a (albeit imperfect) proxy for local discourse. We identify key themes, location and scale, trigger events, actors, authors, and provide initial insights into the problem frames used in these texts. Our findings show distinct differences from the results of environmental media analyses at other scales, including strong individual citizen voices, emphasis on the politics of water, and rare use of language that accords with global environmental discourses; this also differs from results based on interviews and ethnographies at the local scale. Our findings raise important questions about the resonance of global discourse with local views and practices and how local discourses are produced, and suggest a need to more carefully examine the myriad ways of talking about justice and the environment at different scales and through different methodologies.     

Regulating oil and gas facility stormwater discharge: An assessment of surface impoundments,spills, and permit compliance

Contaminated stormwater runoff from oil and gas (O&G) operations can pose a significant threat to surface waters. The purpose of this study is to examine the extent of this threat and identify more specific permitting requirements to protect surface waters. To better understand the extent of the threat, this paper identified and characterized the use of waste surface impoundments at O&G facilities as well as the threat level from O&G spills in California. To assess the efficacy of the current federal and California state-permitting regime, the paper evaluated stormwater permit compliance in two California counties. It also reviewed selected spill cases and associated Spill Prevention, Control and Countermeasure Plans and Stormwater Pollution Prevention Plans to identify the adequacy of current industry practices.The analysis showed that contaminated stormwater from O&G facilities can be better regulated. The U.S. Environmental Protection Agency does not require O&G facilities to file for an Industrial Stormwater General Permit even though many O&G industry practices have the potential to contaminate stormwater runoff from the site. When O&G facilities discharge a Reportable Quantity of a hazardous chemical or violate a water quality standard, they are required to enroll in the National Pollutant Discharge Elimination System permit program. Spills, although not a direct indication of stormwater runoff, can highlight polluted runoff discharges that should have been regulated. Medium and large spills that reached waterways were such a risk for which operators did not file for a permit. In California new filing requirements for the oil and gas industry require all facilities that discharge stormwater that has come into contact with any overburden, raw material, or intermediate products located on the site, to file for an Industrial Stormwater General Permit. As this study showed, there has been an increase in enrollment since the enactment of the new requirements. Having all facilities enroll in the general permit program, as done in California, would require minimum monitoring and maintenance that could help prevent spills. A further step to ensure better protocol is to require specific pollution control practices in addition to the current general permit requirements. Such permitting regimes not only can be implemented on the US federal level but also internationally.     

Regulating groundwater use: The challenges of policy implementation in Guanajuato,Central Mexico

Around the world it has proven very difficult to develop policies and interventions that ensure socio-environmentally sustainable groundwater use and exploitation. In the state of Guanajuato, Central Mexico, both the national government and the decentralized state government have pursued to regulate groundwater use through direct state control, groundwater markets, energy pricing, and user self-regulation. We present and analyze these regulatory mechanisms and their outcomes in the field. We argue that the close interdependencies of these regulatory mechanisms have pre-empted the effectiveness of these policy instruments as well as that of other measures aimed at reducing groundwater use in order to advance towards sustainable exploitation levels.     

Butachlor Degradation in Tropical Soils: Effect of Application Rate,Biotic-Abiotic Interactions and Soil Conditions

The degradative characteristics of butachlor (N-Butoxymethyl-2-chloro-2′,6′-diethyla- cetanilide) were studied under controlled laboratory conditions in clay loam alluvial (AL) soil (Typic udifluvent) and coastal saline (CS) soil (Typic endoaquept) from rice cultivated fields. The application rates included field rate (FR), 2-times FR (2FR) and 10-times FR (10FR). The incubation study was carried out at 30°C with and without decomposed cow manure (DCM) at 60% of maximum water holding capacity (WHC) and waterlogged soil condition. The half-life values depended on the soil types and initial concentrations of butachlor. Butachlor degraded faster in AL soil and in soil amended with DCM under waterlogged condition. Microbial degradation is the major avenue of butachlor degradation from soils.