Nitrate reductase for nitrate analysis in water

Nitrate analysis in water is one of the most frequently applied methods in environmental chemistry. Current methods for nitrate are generally based on toxic substances. Here, we show that a viable alternative method is to use the enzyme nitrate reductase. The key to applying this Green Chemistry solution for nitrate analysis is plentiful, inexpensive, analytical grade enzyme. We demonstrate that recombinant Arabidopsis nitrate reductase, expressed in the methylotrophic yeast Pichia pastoris, is a highly effective catalyst for nitrate analysis at 37°C. Recombinant production of enzyme ensures consistent quality and provides means to meet the needs of environmental chemistry.     

Investigating the sources of the mutagenic activity found in a river using the Salmonella assay and different water extraction procedures

In the routine São Paulo state (Brazil) surface water quality-monitoring program, which includes the Salmonella microsome mutagenicity assay as one of its parameters, a river where water is taken and treated for drinking water purposes has repeatedly shown mutagenic activity. A textile dyeing facility employing azo-type dyes was the only identifiable source of mutagenic compounds. We extracted the river and drinking water samples with XAD4 at neutral and acidic pH and with blue rayon, which selectively adsorbs polycyclic compounds. We tested the industrial effluent, raw, and treated water and sediment samples with YG1041 and YG1042 and compared the results with the TA98 and TA100 strains. The elevated mutagenicity detected with YG-strains suggested that nitroaromatics and/or aromatic amines were causing the mutagenicity detected in the samples analyzed. Positive responses for the blue rayon extracts indicated that mutagenic polycyclic compounds were present in the water samples analyzed. The mutagen or mixture of mutagens present in the effluent and water samples cause mainly frameshift mutations and are positive with and without metabolic activation. The Salmonella assay combined with different extraction procedures proved to be very useful in the identification of the origin of the pollution and in the identification of the classes of chemical compounds causing the mutagenic activity in the river analyzed.     

富营养化供水中的藻类控制与去除

综述了富营养化的供水原水中，藻类控制与去除方法的国内外研究进展。首先扼要介绍藻类过度繁殖对原水处理工艺的诸多影响。然后叙述富营养化湖泊中，藻类生物量的控制方法和给水原水处理的藻类去除工艺，并就此技术现状提出若干结论和建议。     

流动注射荧光猝灭法测定环境水样中痕量磷

在硫酸溶液中，P(Ⅴ)与钼酸盐、钒酸盐生成磷钼钒杂多酸，使罗丹明6G荧光猝灭，据此建立了测定痕量磷的流动注射荧光猝灭方法。方法的测定范围为2～90μg／L,进样频率为35样/h。采用本法测定环境水样中痕量磷，结果令人满意。     

里杜湖富营养化综合评价及预测

根据里杜湖水库的水质监测及水文资料，选择评分法结合生物优势种评价法以及“源－效应”预测模型对里杜湖水库水质的营养状态进行综合评价及预测。结果表明：里杜湖水库水质已轻度富营养化，并有逐年加重的趋势。旨在正确评价和预测里杜湖水库营养状态，并为同类水库的营养状态评价及预测提供参考和借鉴，从而为水库水质管理及富营养化的控制提供科学依据。     

城市水源地突发性水污染事件研究述评

日益恶化的突发性水污染事件始终是国内外大多数城市水源地安全和城市供水安全的重要威胁。从城市水源地突发性污染事件的风险源、水源地易损性、应急评估和预警技术及应急机制4个方面综述了国内外城市水源地突发性污染事件的研究进展。述评认为，应关注城市水源地的特殊易损性及突发性污染的威胁，重点研究水源地突发性污染事件的快速评估和预警技术及应急机制，全面开展城市水源地突发性污染事件的基础和实例研究。     

WebGIS与一维水质模型的集成研究

结合江苏省环保信息系统的建设，讨论WebGIS与一维水质模型集成的关键技术问题，阐明模型计算中评价河段截取、空间离散及空间数据重采样的方法和过程，对实现水环境模型的跨平台操作和数据信息共享作出了尝试，设计了基于浏览器方式的集成系统。     

东深源水生物预处理工程挂膜启动过程水质净化效果变化的分析

东深源水生物预处理工程挂膜启动约需 2 5d ,比优化试验增加 10d左右 ,本文介绍了挂膜启动过程氨氮、CODMn去除效果和填料上生物膜变化的特点。由氨氮去除率 (≥ 5 0 .0 % )、生物膜形状、生物膜上动物种类和数量可判断挂膜启动过程是否完成 ,气温低是该工程挂膜启动过程时间较长的主要原因     

Relating cadmium concentrations in three macrophyte-associated freshwater invertebrates to those in macrophytes,water and sediments

We found that Cd concentrations in three species of macrophyte-associated invertebrates (the gastropods Bithynia tentaculata and Physa gyrina, and the amphipod Gammarus fasciatus) collected at twenty sites along the St. Lawrence River were correlated with Cd concentrations in their main food source, i.e. macrophytes and associated periphyton. Cd in these invertebrates was not significantly correlated with Cd concentrations in the sediments (even when corrections for iron oxide or organic carbon content were applied) or to calculated free-cadmium concentrations in the water. Cd levels in juveniles of B. tentaculata were very tightly linked to Cd concentrations in macrophytes, which is consistent with the close relationship that these organisms have developed with macrophytes. Linear models predicting Cd levels in juveniles of B. tentaculata from Cd levels in different macrophyte species were strong (R2 = 0.69-0.90). Analysis of covariance on these models showed no statistical difference of slope or intercept for any of the macrophyte species, except Vallisneria americana. We suggest that the macrophyte-periphyton complex is a key link in the transfer of cadmium to some aquatic invertebrates in the littoral zone of the St. Lawrence River and that macrophytes and their associated epiphytes should also be used as biomonitors.     

Environmental impact of two successive chemical treatments in a small shallow eutrophied lake: Part I. Case of aluminium sulphate

This paper deals with the efficiency and effects of addition of aluminium sulphate on soft water quality of a shallow eutrophic lake. Almost all the controlled variables improved with treatment, especially nutrient concentrations such as soluble reactive phosphorus (SRP) and transparency. However, aluminium sulphate was not added in sufficient quantity to reduce the total phosphorus content. SRP concentration was significantly reduced in the short term. Moreover, external loading of phosphorus was high and not taken into account by the in-lake treatments. Finally, resuspension of sediment (polymictic lake) removed the alum hydroxide layer on the sediment surface, which reduced treatment effectiveness. No significant pH decrease was noted following alum addition. According to bibliographical toxicological data, monomeric aluminium content does not show any toxic effect on aquatic fauna and flora. In spite of low SRP in the water column, the treatment did not prevent appearance of Microcystis sp. colony (> 10 colony per ml) approximately 30 days after alum application.