细节决定成败——《DNA的粗提取与鉴定》实验失败原因

一件事情能否顺利地做成功,取决于实际操作时每一个细节的成败。大到航天飞机的发射,小到有关一个知识的实验。     

太白山古冰川遗迹资源与旅游可持续开发

太白山是我国东部古冰川地貌保存较好的山地.该冰川遗迹面积较大、规模壮观、形态保存完整,主要分布在垂直带谱显著的山地顶部,景观十分奇特.在分析冰川遗迹形成的地质基础上,探讨了冰川地貌的主要分布类型,评价了冰川地质遗迹的资源特点,提出了旅游可持续开发的一些建议.     

江南水乡古镇旅游开发战略初探--浙江乌镇实证分析

我国长江以南地区分布着为数众多的水乡古镇,它们多以水建镇,以水成市,创造了人类与自然和谐相处的居住环境．具有较高社会学、经济学、历史学、古建筑学等方面研究价值。20世纪80年代以来,水乡古镇旅游日益受到人们青睐,并已成为华东地区旅游的特色品牌之一。然而,随着旅游的发展,一些问题也逐渐暴露出来。浙江乌镇历史悠久．人文荟萃,为浙江省历史文化名镇。其优美的人居环境、深厚的文化底蕴,体现了较高的资源品味;优越的地理区位、精品战略和高投人高产出战略的实施,使得乌镇旅游开发在较短时间内取得了巨大的成功,经济、社会效益显著．知名度迅速扩大,乌镇也因此成为旅游界一匹异军突起的“黑马”,其发展倍受国内外游客及历史文化界人士的瞩目。同时由于开发历史较短,乌镇旅游开发还存在着旅游产品单一、缺乏亮点和特色、社区参与不够、旅游设施难以满足旅游快速发展的需要等现实问题。鉴于此,对江南水乡古镇旅游开发战略进行了初步探讨。     

醛类化合物-DNA加合物结构研究进展

综述了环境中常见的7种醛类污染物与DNA形成加合物的结构研究现状,包括单核苷酸-醛试管反应、DBA-醛试管反应、细胞培养、动物体以及人体内所产生的加合物结构的研究方法与结论,并对加合物结构研究的难点和发展方向进行了探讨。     

重金属铅,镉,锌对小麦DNA构象的影响

采用紫外吸收谱法，研究了不同浓度铅，镉，锌对小麦ＤＮＡ构象的影响。结果显示重金属铅，镉，锌均可影响小麦ＤＮＡ构象，使其紫外吸收峰值发生变化。其中，铅离子对小麦ＤＮＡ构象影响最大，５０μｇ／ｍｌ＾ｐｂ＾２＋处理使小麦出现明显减色反应，１０００μｇ／ｍｌｐｂ＾２＋处理使其沉淀析出；镉离子对小麦ＤＮＡ构象影响不大，可使其产生轻微减色反应，低浓度锌离子使小麦ＤＮＡ发生增色反应，而高浓度使小麦ＤＮＡ发生减色     

用增强灵敏度的~32P后标记法测定DNA-环氧苯乙烯加合物

DNA加合物是具有亲电性化学致癌物与DNA形成共价相联的化合物,是化学致癌物损伤DNA的主要形式,能够灵敏准确地分析DNA加合物的方法是研究DNA损伤     

Effects of temperature on UV-B-induced DNA damage and photorepair in Arabidopsis thaliana

DNA damage in the form of cyclobutane pyrimidine dimers(CPDs) and (6-4) photopreducts(6-4PPs) induced by UV-B radiation in Arabidopsis thaliana at different temperatures was investigated using ELISA with specific monoclonal antibodies. CPDs and 6-4PPs increased during 3 h UV-B exposure, but further exposure led to decreases. Contrary to the commonly accepted view that DNA damage induced by UV-B radiation is temperature-independent because of its photochemical nature, we found UV-B-induction of CPDs and 6-4PPs in Arabidopsis to be slower at a low than at a high temperature. Photorepair of CPDs at 24℃ was much faster than that at 0℃ and 12℃,with 50% CPDs removal during 1 h exposure to white light. Photorepair of 6-4PPs at 12℃ was very slow as compared with that at 24℃,and almost no removal of 6-4PPs was detected after 4 h exposure to white light at 0℃. There was evidence to suggest that temperaturedependent DNA damage and photorepair could have important ecological implications.     

Degradation of phenol in an upflow anaerobic sludge blanket(UASB) reactor at ambient temperatureKE

A synthetic wastewater containing phenol as sole substrate was treated in a 2.8 L upflow anaerobic sludge blanket(UASB) reactor at ambient temperature. The operation conditions and phenol removal efficiency were discussed, microbial population in the UASB sludge was identified based on DNA cloning, and pathway of anaerobic phenol degradation was proposed. Phenol in wastewater was degraded in an UASB reactor at loading rate up to 18 gCOD/(L·d), With a 1:1 recycle ratio, at 26(1℃, pH 7.0-7.5. An UASB reactor was able to remove 99% of phenol up to 1226 mg/L in wastewater with 24 h of hydraulic retention time(HRT). For HRT below 24 h, phenol degradation efficiency decreased with HRT, from 95.4% at 16 h to 93.8% at 12 h. It further deteriorated to 88.5% when HRT reached 8 h. When the concentration of influent phenol of the reactor was 1260 mg/L(corresponding COD 3000 mg/L), with the HRT decreasing(from 40 h to 4 h, corresponding COD loading increasing), the biomass yields tended to increase from 0.265 to 3.08 g/(L·d). While at 12 h of HRT, the biomass yield was lower. When HRT was 12 h, the methane yield was 0.308 L/(gCOD removed), which was the highest. Throughout the study, phenol was the sole organic substrate. The effluent contained only residual phenol without any detectable intermediates, such as benzoate, 4-hydrobenzoate or volatile fatty acids(VFAs). Based on DNA cloning analysis, the sludge was composed of five groups of microorganisms. Desulfotomaculum and Clostridium were likely responsible for the conversion of phenol to benzoate, which was further degraded by Syntrophus to acetate and H2/CO2. Methanogens lastly converted acetate and H2/CO2 to methane. The role of epsilon-Proteobacteria was, however, unsure.