LIMITING NUTRIENT DETERMINATION IN LOTIC ECOSYSTEMS USING A QUANTITATIVE NUTRIENT ENRICHMENT PERIPHYTOMETER1

ABSTRACT: The decline of water quality in United States’ lotic ecosystems (streams and rivers) has been linked to nonpoint source nutrient loading (U.S. EPA, 1990). Determining limiting nutrients in streams is difficult due to the variable nature of lotic ecosystems. We developed a quantitative passive diffusion periphyton nutrient enrichment system, called the Matlock Periphytometer, to measure the response of attached algae (periphyton) to nutrient enrichment. The system is simple to build and provides quantitative nutrient enrichment of a surface for periphytic growth. The periphyton grow on a glass fiber filter, which allows complete recovery of periphyton for chlorophyll a analysis. A 14-kilodalton dialysis membrane was used as a biofilter to prevent bacterial and algal contamination of the nutrient solution. We determined the rates of diffusion of nitrogen and phosphorus ions across the Matlock Periphytometer's dialysis membrane and glass fiber filter over a 21-day period (42 and 22 μg/cm²/hr, respectively). We used the Matlock Periphytometer to determine the limiting nutrient in a woodland stream. Six replicates each of a control, nitrogen, and phosphorus treatment were placed in the stream for 14 days. The results indicated that phosphorus was the limiting nutrient in the stream for the period and location sampled.     

REGIONAL SCALE TREND MONITORING OF INDICATORS OF TROPHIC CONDITION OF LAKES1

ABSTRACT: The U.S. Environmental Protection Agency has proposed a sample survey design to answer questions about the ecological condition and trends in condition of U.S. ecological resources. To meet the objectives, the design relies on a probability sample of the resource population of interest (e.g., a random sample of lakes) each year on which measurements are made during an index period. Natural spatial and temporal variability and variability in the sampling process all affect the ability to describe the status of a population and the sensitivity for trend detection. We describe the important components of variance and estimate their magnitude for indicators of trophic condition of lakes to illustrate the process. We also describe models for trend detection and use them to demonstrate the sensitivity of the proposed design to detect trends. If the variance structure that develops during the probability surveys is like that synthesized from available databases and the literature, then the trends in common indicators of trophic condition of the specified magnitude should be detectable within about a decade for Secchi disk transparency (0.5–1 percentiyear) and total phosphorus (2–3 percent/year), but not for chlorophyll-a (> 3–4 percent/year), which will take longer.     

CONTROL OF SUSPENDED SOLIDS AND PHYTOPLANKTON WITH FISHES AND A MUSSEL1

ABSTRACT: Filtering efficiency of the fathead minnow (Pimephales promelas), gizzard shad (Dorosoma cepedianum), carp (Cyprinus carpio), and a freshwater mussel (Elliptio coinpianata) was measured in field and laboratory trials to assess the ability of each species to control phytoplankton and suspended solid densities. All fish species tested were ineffective filterers and generally increased, rather than suppressed, algal and suspended solid concentrations. Filtering efficiencies of fish varied between -354 and 84 percent, depending on the size, shape, abundance, palatability, composition, and resistance to digestion of the particles. Because of poor filtering abilities, unpredictable feeding habits, and sensitivity to stress, the fish species examined are not effective biological controls in waste lagoons. In contrast, the freshwater mussel Elliptio was a highly effective control organism, averaging 66 percent filtering efficiency over a wide size range of algal and suspended particles. Filtration efficiency was positively correlated with mussel density. Elliptio was efficient at filtering small particles, which are particularly difficult to remove. Mean filtration rates ranged from 53 to 134 ml/mussel/h depending on the algal species consumed and algal densities (range 50–180,000 cells/ml) and 3 mg/L/mussel/h on suspended solids (range 14 to 112 mg/L). Water clarification was facilitated by both direct consumption and pseudofeces deposition. Elliptio and probably other mussel species can effectively control algae and suspended solids in wastewater lagoons and eutrophic lakes, if environmental conditions, especially dissolved oxygen levels, are suitable (>5 mg/L) for their survival.     

EFFICACY AND COST OF AQUATIC WEED CONTROL IN SMALL PONDS'

ABSTRACT: Twenty, 0.2 hectare ponds were utilized for a four-year evaluation of three aquatic vegetation control techniques: a combination of inorganic fertilization and mechanical harvesting, aquatic herbicides, and grass carp (Ctenopharyngodon idella). Ponds used for herbicide and grass carp treatments were managed at three levels of aquatic vegetation (none, 40 and 70 percent plant occupation). Submersed vegetation was not controlled with inorganic fertilization. Vegetation levels were maintained for less than 30 days after mechanicai harvesting. Submersed macrophytes were not completely eliminated with herbicides, but the herbicide treatments utilized were effective at maintaining aquatic vegetation above 30 percent pond volume occupation. Grass carp consumed all species of submersed vegetation at the stocking densities used in this study; therefore, planned levels of submersed macrophytes were not maintained. Grass carp did not consume all floating leaf vegetation, but after four years some grass carp ponds did have lower densities of floating leaf plants. Fertilization costs were $608/hectare/year, and mechanical harvesting costs were $1979/hectare/year, resulting in a total aquatic vegetation treatment cost of $2587/hectare/ year. Herbicide costs for the different treatment levels ranged from $417/hectare/year to $1339/hectare/year over the four-year period. Grass carp were the most economical vegetation control measure tested, with costs ranging from $159/hectare/year to $248/hectare/year for the four-year study.     

THE CASE FOR DIRECT MEASUREMENT OF ENVIRONMENTAL RESPONSE TO HAZARDOUS MATERIALS1

ABSTRACT: Direct measurement of response thresholds of complex biological systems to toxic chemicals is now possible using microcosms and other simulation techniques. Extrapolation to no-observable response using application factors and data from single species toxicity tests is less precise than direct measurements.     

TOXIC EFFECTS OF PETROLEUM AND SHALE JP-4 AND JP-8 AVIATION FUELS ON FATHEAD MINNOWS1

ABSTRACT: Static 96-hour median lethal concentrations were determined for the water-soluble fraction (WSF) of both petroleum-derived and shale-derived avaiation fuels (JP-4 and JP-8) using fathead minnows. JP-8 was more toxic than JP-4 except for one shale JP-4 sample that was as toxic as the JP-8. Petroleum and shale JP-8 were similar in toxicity. The toxicity of shale JP-4 was less clear. Shale JP-4 from three vendor sources revealed differing toxicity values. One shale JP-4 sample was more toxic and one less toxic than its petroleum analogue, with the third being equally toxic. Toxicity of the fuels may be enhanced by compounds in the WSF that correspond to chemicals containing 10 or more carbon atoms.     

THERMAL AND DISSOLVED OXYGEN CHARACTERISTICS OF A SOUTH CAROLINA COOLING RESERVOIR1

ABSTRACT: Temperature and dissolved oxygen concentrations were measured monthly from January 1971 to December 1982 at 1-m depth intervals at 13 stations in Keowee Reservoir in order to characterize spatial and temporal changes associated with operation of the Oconee Nuclear Station. The reservoir water column was i to 4°C warmer in operational than in non-operational years. The thermo-dine was at depths of 5 to 15 m before the operation of Oconee Nuclear Station, but was always below the upper level of the intake (20 m) after the station was in full operation; this suggests that pumping by the Oconee Nuclear Station had depleted all available cool hypolimnetic water to this depth. As a result summer water temperatures at depths greater than 10 m were usually 10°C higher after plant operation began than before. By fall the reservoir was nearly homothemious to a depth of 27 m, where a thermocine developed. Seasonal temperature profiles varied with distance from the plant; a cool water plume was evident in spring and a warm water plume was present in the summer, fall, and winter. A cold water plume also developed in the northern section of the reservoir due to the operation of Jocassee Pumped Storage Station. Increases in the mean water temperature of the reservoir during operational periods were correlated with the generating output of the power plant. The annual heat load to the reservoir increased by one-third after plant operations began. The alteration of the thermal stratification of the receiving water during the summer also caused the dissolved oxygen to mix to greater depths.     

RAPID WATER TABLE RESPONSES TO RAINFALL IN A NORTHERN PEATLAND ECOSYSTEM1

ABSTRACT: Two types of rapid water table responses to rain were observed in a northern Michigan peatland. The first, called the Lisse effect, occurred during rains of high intensity when the infiltrating water acted as a tightly closing lid that forced the water table to rise to the level required to compensate for the pressure increase. The second, called the Wieringermeer effect, was a rapid rise of the water table to the surface due to the conversion of capillary to phreatic water and was always followed by an equally rapid decline after cessation of the rainfall. We simulated these phenomena in the laboratory and estimated the critical parameters that determine their occurrence. The recognition of the importance of the capillary fringe is essential in evaluating the role of wetlands in flood control and in wastewater treatment.     

Ecotoxicological implications of aquatic disposal of coal combustion residues in the United States: a review

We provide an overview of research related to environmental effects of disposal of coal combustion residues (CCR) in sites in the United States. Our focus is on aspects of CCR that have the potential to negatively influence aquatic organisms and thehealth of aquatic ecosystems. We identify major issues of concern, as well as areas in need of further investigation.Intentional or accidental release of CCR into aquatic systemshas generally been associated with deleterious environmental effects. A large number of metals and trace elements are presentin CCR, some of which are rapidly accumulated to high concentrations by aquatic organisms. Moreover, a variety of biological responses have been observed in organisms following exposure to and accumulation of CCR-related contaminants. In some vertebrates and invertebrates, CCR exposure has led to numerous histopathological, behavioral, and physiological (reproductive, energetic, and endocrinological) effects. Fish kills and extirpation of some fish species have been associatedwith CCR release, as have indirect effects on survival and growth of aquatic animals mediated by changes in resource abundance or quality. Recovery of CCR-impacted sites can be extremely slow due to continued cycling of contaminants withinthe system, even in sites that only received CCR effluents forshort periods of time. The literature synthesis reveals important considerations for future investigations of CCR-impacted sites. Many studies have examined biological responses to CCR with respect to Se concentrations and accumulation because of teratogenic andreproductively toxic effects known to be associated with thiselement. However, the complex mixture of metals and traceelements characteristic of CCR suggests that biologicalassessments of many CCR-contaminated habitats should examine avariety of inorganic compounds in sediments, water, and tissuesbefore causation can be linked to individual CCR components. Most evaluations of effects of CCR in aquatic environments havefocused on lentic systems and the populations of animalsoccupying them. Much less is known about CCR effects in loticsystems, in which the contaminants may be transported downstream,diluted or concentrated in downstream areas, and accumulated bymore transient species. Although some research has examinedaccumulation and effects of contaminants on terrestrial and avianspecies that visit CCR-impacted aquatic sites, more extensiveresearch is also needed in this area. Effects in terrestrial orsemiaquatic species range from accumulation and maternal transferof elements to complete recruitment failure, suggesting that CCReffects need to be examined both within and outside of theaquatic habitats into which CCR is released. Requiring specialattention are waterfowl and amphibians that use CCR-contaminatedsites during specific seasons or life stages and are highlydependent on aquatic habitat quality during those periods.Whether accidentally discharged into natural aquatic systems or present in impoundments that attract wildlife, CCR appears topresent significant risks to aquatic and semiaquatic organisms. Effects may be as subtle as changes in physiology or as drasticas extirpation of entire populations. When examined as a whole,research on responses of aquatic organisms to CCR suggests thatreducing the use of disposal methods that include an aquaticslurry phase may alleviate some environmental risks associatedwith the waste products.     

Assessing the Effects of Non-Point Source Pollution on American Samoa’s Coral Reef Communities

Surveys were completed on Tutuila Island, American Samoa, to characterize reef development and assess the impacts of non-point source pollution on adjacent coral reefs at six sites. Multivariate analyses of benthic and coral community data found similar modern reef development at three locations; Aoa, Alofau, and Leone. These sites are situated in isolated bays with gentle sloping foundations. Aoa reefs had the highest estimates of crustose coralline algae cover and coral species richness, while Leone and Alofau showed high abundances of macroalgae and Porites corals. Aoa has the largest reef flat between watershed discharge and the reef slope, and the lowest human population density. Masefau and Fagaalu have a different geomorphology consisting of cemented staghorn coral fragments and steep slopes, however, benthic and coral communities were not similar. Benthic data suggest Fagaalu is heavily impacted compared with all other sites. Reef communities were assessed as bio-criteria indicators for waterbody health, using the EPA aquatic life use support designations of (1) fully supportive, (2) partially supportive, and (3) non-supportive for aquatic life. All sites resulted in a partially supportive ranking except Fagaalu, which was non-supportive. The results of this rapid assessment based upon relative benthic community measures are less desirable than long-term dataset analyses from monitoring programs, however it fills an important role for regulatory agencies required to report annual waterbody assessments. Future monitoring sites should be established to increase the number of replicates within each geological and physical setting to allow for meaningful comparisons along a gradient of hypothesized pollution levels.