Isolation and characterization of a strain with high microbial attachment in aerobic granular sludge

Aerobic granule is a special microbial aggregate associated with biofilm structure. The formation of aerobic granular sludge is primarily depending on its bacterial community and relevant microbiological properties. In this experiment, a strain with high microbial attachment was isolated from aerobic granular sludge, and the detailed characteristics were examined. Its high attachment ability could reach 2.34 (OD_600nm), while other low attachment values were only around 0.06-0.32, which indicated a big variation among the different bacteria. The strain exhibited a very special morphology with many fibric fingers under SEM observation. A distinctive behaviour was to form a spherical particle by themselves, which would be very beneficial for the formation and development of granular sludge. The EPS measurement showed that its PN content was higher than low attachment bacteria, and 3D-EEM confirmed that there were some different components. Based on the 16S rRNA analysis, it was identified to mostly belong to Stenotrophomonas. Its augmentation to particle sludge cultivation demonstrated that the strain could significantly promote the formation of aerobic granule. Conclusively, it was strongly suggested that it might be used as a good and potential model strain or chassis organism for the aerobic granular sludge formation and development.     

废旧塑料浮选分离中气泡黏附行为的影响

塑料浮选作为一种基于表面活性剂的废旧塑料分选技术,是解决废旧塑料环境污染的有效方法. 为深入考察塑料浮选过程中气泡的黏附行为及影响因素,运用高速摄影技术结合气泡黏附时间模型,对比分析纯水中气泡与亲/疏水材料的黏附效果,重点比较不同溶液〔纯水、鼠李糖脂和SDS(十二烷基硫酸钠)溶液〕中气泡与疏水材料的黏附时间变化,并探讨其影响机制. 结果表明:气泡在纯水中只与疏水材料发生黏附,表面活性剂及其浓度对气泡与疏水材料的黏附时间影响显著. 在c(鼠李糖脂)和c(SDS)(二者均为0.03 mmol/L)均较低的溶液中,气泡与疏水材料的黏附时间由纯水中的158 ms分别降至120和140 ms,而二者较高(10.00 mmol/L)时黏附时间分别增至1 134和338 ms. 基于黏附时间模型分析表明,黏附时间是气泡尺寸、接触角、溶液黏度及表面张力等参数的函数,通过控制这些参数可以调控气泡在目标塑料表面的黏附时间,实现浮选效率的优化.      

Transparent exopolymer particles (TEPs)-associated protobiofilm: A neglected contributor to biofouling during membrane filtration

• Bacteria could easily and quickly attached onto TEP to form protobiofilms. • TEP-protobiofilm facilitate the transport of bacteria to membrane surface. • More significant flux decline was observed in the presence of TEP-protobiofilms. • Membrane fouling shows higher sensitivity to protobiofilm not to bacteria level. Transparent exopolymer particles (TEPs) are a class of transparent gel-like polysaccharides, which have been widely detected in almost every kind of feed water to membrane systems, including freshwater, seawater and wastewater. Although TEP have been thought to be related to the membrane fouling, little information is currently available for their influential mechanisms and the pertinence to biofouling development. The present study, thus, aims to explore the impact of TEPs on biofouling development during ultrafiltration. TEP samples were inoculated with bacteria for several hours before filtration and the formation of “protobiofilm” (pre-colonized TEP by bacteria) was examined and its influence on biofouling was determined. It was observed that the bacteria can easily and quickly attach onto TEPs and form protobiofilms. Ultrafiltration experiments further revealed that TEP-protobiofilms served as carriers which facilitated and accelerated transport of bacteria to membrane surface, leading to rapid development of biofouling on the ultrafiltration membrane surfaces. Moreover, compared to the feed water containing independent bacteria and TEPs, more flux decline was observed with TEP-protobiofilms. Consequently, it appeared from this study that TEP-protobiofilms play a vital role in the development of membrane biofouling, but unfortunately, this phenomenon has been often overlooked in the literature. Obviously, these findings in turn may also challenge the current understanding of organic fouling and biofouling as membrane fouling caused by TEP-protobiofilm is a combination of both. It is expected that this study might promote further research in general membrane fouling mechanisms and the development of an effective mitigation strategy.     

Container vs. dacha: The psychological effects of temporary housing characteristics on earthquake survivors

This research investigated whether the psychological wellbeing of earthquake survivors two years after the traumatic event would vary as a function of type of temporary housing. A comparison of people assigned to containers, converted into mobile homes, vs. wooden dachas vs. a control group, that had not lost their homes in the earthquake, revealed reliable effects of housing type. Compared to those in containers, earthquake victims assigned to dachas were more satisfied with and more attached to their temporary homes and reported greater psychological wellbeing (fewer psychological stress symptoms, less discomfort and a lesser feeling of being dominated by the situation). Also, home attachment and satisfaction with one's home mediated the psychological wellbeing, suggesting that the attitudes towards different types of temporary housing play a causal role in post-traumatic stress reactions. Practical implications for emergency interventions following natural disasters are discussed.     

Visitor Response to Management Regulations—A Study Among Recreationists in Southern Norway

/ The objectives of nature area management are often twofold: To protect the natural environment and to facilitate recreational use. In order to maintain the natural setting, it is sometimes necessary to regulate the recreational use of an area. In deciding on a management action, one problem can be the lack of knowledge about the effects of management actions on visitors. In order to enhance the knowledge base for future management practices, this study empirically evaluates the effects of management regulations in a Norwegian nature area. In this area camping outside commercial campgrounds was restricted in 1992. The management regulations seems to have influenced the use of Sjodalen for camping in several ways. The number of campers using the area has decreased. The user composition seems to have changed, with new campers in the area after the regulation being more tolerant of human influence on the natural environment than the campers before the regulations. In addition, a considerable proportion of the existing users ceased to stay overnight in Sjodalen, totally or partly due to the regulations. The behavioral response among existing users is related both to environmental preferences and place attachment. Implications for management and future research studies on impact assessment in general, and displacement specifically, are discussed.KEY WORDS: Outdoor recreation; Management regulations; Behavioral response; Displacement, Place attachment     

Thermal analysis of polyethylene glycol: evolved gas analysis with ion attachment mass spectrometry

The thermal decomposition of polyethylene glycol was investigated by using a technique combining evolved gas analysis (time-resolved pyrolysis) with ion-attachment mass spectrometry. This technique allows the detection of intact pyrolysis products and, therefore, offers the opportunity for direct real-time monitoring of thermal by-products. Unstable products can thus be detected; for instance, many highly reactive organic peroxides, such as CH₃OOH and HOCH₂OOH, were found in this study. Classification analysis revealed 10 major compositional formulas among the product species: C_nH_2n+2O, C_nH_2n+2O₂, C_nH_2n+2O₃, C_nH_2n+2O₄, C_nH_2n+2O₅, C_nH_2n+2O₆, C_nH_2n+2O₇, C_nH_2nO, C_nH_2nO₂, and HO(CH₂CH₂O)_nH ethylene glycol oligomers. The Li⁺ ion adduct mass spectra showed a characteristic profile in terms of both the appearance of unique components and the distribution of pyrolysis products. Among the products of the thermal decomposition of PEG, formaldehyde (HCHO) and organic peroxides were particularly interesting. Formaldehyde, one of the 10 most abundant products, is a known human carcinogen. The detection of peroxides suggests that they may form during the incineration of PEG, which may have important environmental implications. The existence of peroxide products may have implications for chemical evolution in incinerator systems.     

The measurement of place attachment: Personal,community, and environmental connections

Place attachment has been researched extensively in the social and behavioural sciences over the past two decades. However, it is challenging for researchers to assimilate the mixed messages presented in the place attachment literature and to understand the multiple place attachment terms. In this study, a four-dimensional model of rural landholder attachments to their natural resource management region was conceptually and empirically developed with the aim of developing an integrated approach to the measurement of place attachment that clearly distinguishes between different elements of place scholarship. A 29-item place attachment scale with the dimensions of place identity, place dependence, nature bonding, and social bonding was tested on a random sample of rural landholders in the Adelaide and Mount Lofty Ranges in South Australia (N = 320). The majority of respondents were male (69.3%) and the average age was 59 years. The scale was reduced to 20-items and then administered simultaneously and in the same response format to rural landholders in two other areas of South Australia: the Northern and Yorke region (N = 664) and South Australian Murray-Darling Basin region (N = 659). In both studies, the majority of respondents were male (85%) and the average age was 55 years. Exploratory factor and reliability analyses of Adelaide and Mount Lofty Ranges and Northern and Yorke datasets produced a five-dimensional model of place attachment with high reliabilities. Social bonding divided into the constructs of family bonding and friend bonding. The refined five-dimensional model was then examined for convergent validity, with moderate but significant correlations found between individual attachment constructs and dependent variables expected to be related to the construct, such as place identity and length of residence, and nature bonding and time currently spent in nature. We used confirmatory factor analysis to test the goodness-of-fit of the South Australian Murray-Darling Basin (SAMDB) dataset to the proposed five-dimensional model and then compared its fit to the traditional two-dimensional model of place identity and place dependence. The five-dimensional model provided moderate fit for the SAMDB data. We conclude with a discussion of the validity and reliability of the five-dimensional model and its future role in place attachment research.     

Disruption to place attachment and the protection of restorative environments: A wind energy case study

Deepening understanding of public responses to large-scale renewable energy projects is of academic and practical importance, given policies to lessen fossil fuel use in many countries. Although the NIMBY (Not In My Back Yard) concept is commonly used to explain public opposition, the concept has been extensively critiqued. This study applies an alternative approach based upon the notion of disruption to place attachment, and the theory of social representations, with a focus upon the symbolic meanings associated with a proposed project and the places affected by it. Empirical data is provided from a case study of a proposed 750 MW offshore wind farm in North Wales, using group discussions and questionnaires distributed to local residents in two coastal towns (n = 488). Results indicate significant differences between each town's residents in their responses to the project, and how opposition arises from nature/industry symbolic contradictions: between a place represented in terms of scenic beauty that provides a restorative environment for residents and visitors, and a wind farm that will industrialise the area and ‘fence’ in the bay. In one of the towns, the data suggests that contradiction between project and place was experienced as a threat to identity for those with strong place attachment, leading to negative attitudes and oppositional behaviour. Levels of trust in key actors moderated the relation between place attachment and negative attitudes to the wind farm. The results provide further evidence of the role of place attachment in shaping so-called ‘NIMBY’ responses to development proposals, and challenge the assumption that offshore wind farms will prove less controversial than those onshore.     

Connecting with Country in Mungo National Park,Australia: a case study to measure the emotional dimension of experience and place attachment

Connecting people with nature improves well-being, but how people connect with natural places is not well documented. We asked 43 people (19 Aboriginal Australians, 24 non-Aboriginal people) about the messages they received from Country during an interactive experience in the remote Mungo National Park, Australia, and analysed the physical senses, emotions and cognitive processes they mentioned. The physical senses mentioned by most respondents were sight, hearing and motion (particularly walking). These senses helped people receive messages from Country and connect with place. We used the primary-process emotional systems of Panksepp [2010. Dialogues in Clinical Neuroscience, 12 (4), 533–545] as a framework to capture the emotional dimension of experience. Most people reported positive emotions; they spoke about being nurtured by the group and the land (CARE), and the intense joy (PLAY) of being part of the community, being on Country and being accompanied by Aboriginal people. However, our results indicate the framework does not capture the breadth of positive emotions, particularly those associated with connection to place and spiritual experiences. Both groups mentioned cognitive processes reflecting their beliefs, existing knowledge, or sharing and acquiring new knowledge. Our results indicate that the emotional dimension of experience has the potential to measure connection to place, and provide a subjective measure of well-being. More research is needed to document this dimension of experience, and how it changes with context. Our case study provides further insight for those who manage protected areas and seek to enrich the experience of visitors.     

The influence of place attachment, and moral and normative concerns on the conservation of native vegetation: A test of two behavioural models

This study examines the influence of place attachment, values, beliefs and personal norms about environmental action on the conservation of native vegetation in two primary production settings in South Australia. We use regression and multiple mediation analyses to test a base model of pro-environmental behaviour which includes variables from value-belief-norm (VBN) theory and then compare it to an expanded model which includes the same variables and five dimensions of place attachment. The expanded model including place attachment explained up to twice the amount of variance in native vegetation planting than the base model when controlling for all variables preceding behaviour, but the overall explanatory power was low (<22%). Place attachment had a stronger influence on the antecedents of behaviour compared with the behaviour itself, particularly nature bonding which was a significant moderate predictor of both personal norms and awareness of consequences in the two study regions. We assert that place attachment has statistically significant direct and indirect effects on variables included in VBN theory. Future studies may reveal stronger effects in settings where there are fewer resource and monetary costs associated with pro-environmental behaviour.