蜡状芽孢杆菌45号固定化细胞脱除酸性红B的研究

研究并比较了蜡状芽孢杆菌45号自然细胞和固定化细胞的某些性质。结果表明,该菌株的两种细胞脱色反应的最适温度均为37℃,热稳定性良好。在50ppm的酸性红B溶液中经37℃保温处理后,两种细胞的脱色酶活性有明显提高。其脱色反应的最适pH值分别为7—10和5—10。固定化细胞在4—6℃的冰箱中保存52天,脱色酶的活性不变。应用固定化细胞连续处理酸性红B溶液,当进水浓度为42.1ppm时,出水平均脱色率可达87％。     

First trimester diagnosis of Gaucher disease in a fetus with trisomy 21

A 38-year-old lady, who had a previous infant with type 2 Gaucher disease, underwent prenatal diagnosis by chorionic villus sampling at 9 weeks' gestation. Results on the fresh villus revealed a 47,XY,+21 karyotype and a marked deficiency (2 per cent of control) of β-glucosidase activity. Following termination, villus material was cultured which initially revealed only a partial enzyme deficiency and a normal female karyotype, i.e., maternal cells. A subsequent culture contained 47,XY, + 21 cells which were deficient in β-glucosidase activity, thus confirming the diagnosis. The results in this interesting case illustrate the potential dangers of maternal cell contamination in cultured villus cells.     

Magnetic-activated cell sorting (MACS) significantly decreases the hybridization efficiency of fluorescence in situ hybridization (FISH)

Fetal cells were enriched from maternal blood using density gradient centrifugation of Histopaque followed by magnetic-activated cell sorting (MACS) to select CD71-positive cells. For each specimen, cells partially purified by Histopaque were split into equal portions, and each portion was subjected to purification by MACS in parallel. Cells before and after MACS were subjected to dual-color fluorescence in situ hybridization (FISH) analysis with X- and Y-chromosome-specific probes. We found that the hybridization rates were decreased by approximately 10% after MACS based on duplicated analysis for each sample. Copyright © 2001 John Wiley & Sons, Ltd.     

Performance and recent improvement in microbial fuel cells for simultaneous carbon and nitrogen removal: A review

Microbial fuel cells (MFCs) have become a promising technology for wastewater treatment accompanying electricity generation. Carbon and nitrogen removal can be achieved by utilizing the electron transfer between the anode and cathode in an MFC. However, large-scale power production and high removal efficiency must be achieved at a low cost to make MFCs practical and economically competitive in the future. This article reviews the principles, feasibility and bottlenecks of MFCs for simultaneous carbon and nitrogen removal, the recent advances and prospective strategies for performance improvement, as well as the involved microbes and electron transfer mechanisms.     

“Housing for all” at the era of financialization; can (post-disaster) cities become truly socially resilient and egalitarian?

This paper examines the realism of the resilience ambition and process of the U.S. housing system, shedding light on its heterogeneity as well as the financialization currently acting as the driving force in real estate production. The resilience ambition leading to enhanced justice and egalitarianism is understood as the provision and maintenance of post-disaster housing for all within an institutionally diverse landscape of housing policy makers and implementers. Particular emphasis is given to the post-Katrina institutional transformations resulting from multifarious interactions between multilevel institutional structures and a diverse landscape of low-income housing policy implementers – referred as social resilience cells (SRCs) in this paper. The nature and level of these transformations determine the degree to which resilience in its heterogeneous form has been incubated in New Orleans. The paper concludes with a discussion on the macro conditions and bottom-linked governance structures under which all SRCs could be better bolstered in a post-financialization, radicalised neowelfare U.S., and which in turn create possibilities for materialising the resilience ambition.     

吸烟烟气对鼠肺细胞膜的损伤和茶多酚的保护作用

本文以香烟气相物质作用鼠肺细胞膜为模型,用脂肪酸自旋标记物5-DOXYL和16DOXYL分别研究膜浅层和深层的动态性质受气相烟的影响,并用紫外可见分光光度法研究气相烟对膜脂的作用。结果发现,在实验的气相烟流量下,香烟气相物质能引发鼠肺细胞膜的脂质过氧化,并且使膜浅层的流动性增大。但对膜深层的动态性质没有明显的改变,如果在鼠肺细胞中预先加入粗晶态或粉态茶多酚。则肺细胞的过氧化和膜的动态性质改变受到抑制,而且这种抑制作用与茶多酚浓度呈量效关系,而茶多酚本身对膜浅层无明显作用,但对膜深层的流动性有一定影响,而且两种茶多酚的作用相似。     

The Nile delta-Alexandria coast: vulnerability to sea-level rise,consequences and adaptation

Like many delta systems, the coastal zoneof the Nile delta has been designated as avulnerable zone to a rising sea level as aconsequence of expected climate changescombined with geological and human factors.In view of the understanding of thesefactors, a degree of vulnerabilityanalysis has been carried out to betterlocate which sectors need to be assessedand adapted to possible sea level rise(SLR) for the Nile delta-Alexandria region of Egypt.Results reveal that not all of the coastalzones of the Nile delta are vulnerable toaccelerated sea-level rise at the samelevel. Based on multiple criteria the Niledelta-Alexandria coast can be categorizedinto vulnerable (30%), invulnerable (55%)and artificially protected coastalstretches (15%). These criteria include:local subsidence or uplifting, relativesea-level rise (RSLR), land topography,width of lagoon barriers, beach-face slope,high-elevated features such as dunes andridges, eroding and accreting coastlinesand protection works.Moreover, this study evaluates thelong-term relative sea-level rise andsubsidence rates along the Nile delta andAlexandria coasts. Statistical analysis oflong-term tide gauge data recorded atAlexandria, Burullus and Port Said yieldsvalues of 1.6, 1.0 and 2.2 mm/yr,respectively. These values of relativesea-level rise and long-term subsidencerate obtained from age-dated sediment coresections are inconsistent: long-termsubsidence appears to be larger (maximum of7 mm/ yr). This discrepancy might beexplained if the subsidence is episodic,and occurs rather abruptly during majorearthquakes that occur every few hundredyears associated with fault trend lines.Rising sea levels could have significantlongterm impacts on the Nile delta,including the distribution of ground watersalinity and erosion of the narrow andlow-lying barriers of the Burullus andManzala lagoons. Adaptive measures alongthe study area particularly those relatedto coastal protective structures are alsoevaluated.     

中国沿海常见棘毒鱼类的毒性研究：日本鬼背鳍棘中Ⅰ型毒腺细？…

皮光、电镜方法研究了日本鬼Ｙｏｕ背鳍棘中Ⅰ型毒腺细胞的形成过程。结果表明：位于背鳍棘两侧纵沟内的毒腺组织周围有较致密的结缔组织的鞘膜包绕，该鞘膜钝性剥离后可见内面光滑，有Ⅰ型和Ⅱ型型毒腺细胞的痕迹。而毒腺组织内下侧与毒棘的骨组织相连处结缔组织疏松，有许多小的梭形细胞；在梭形细胞之间及周围有新形成的幼稚腺细胞，其组织结构类似Ⅰ型毒腺细胞。透射电镜下可见该区域的结缔组织中除结缔组织的细胞及成分以外，有     

The characterization of hCG regulation in cultured human amniotic fluid cells

The media from primary cultures and subcultures of second trimester human amniotic fluid (AF) cells were assayed by radioimmunoassay to quantitate production of human chorionic gonadotropin (hCG). Primary AF cultures produce more hCG per cell than do the corresponding subcultures. Sodium butyrate (2 mM) stimulates AF subcultures to produce 5-13 times more hCG per cell or per mg of cellular protein than do untreated subcultures. This stimulatory effect of sodium butyrate is dose dependent between 0 and 5 mM. Addition of sodium butyrate 24 hours after subculture, while stimulating production of hCG during the subsequent 3 days, also results in fewer cells and less protein per culture. This effect on cell growth is also dose-dependent. Previous investigators have proposed that the stimulation of hCG by sodium butyrate in other types of cell cultures is due to an effect of that agent on culture growth. Therefore, in these studies AF cells are allowed to grow to confluency before sodium butyrate was added. Production of hCG was stimulated by sodium butyrate about four-fold during the next 5 days although no significant changes were observed either in number of cells or amount of cellular protein per culture. These results suggest that stimulation of hCG by sodium butyrate is not dependent on its effect on growth of the cultures.     

Isolating fetal cells in maternal circulation for prenatal diagnosis

Fetal cells unequivocally exist in and can be isolated from maternal blood. Erythroblasts, trophoblasts, granulocytes and lymphocytes have all been isolated by various density gradient and flow sorting techniques. Chromosomal abnormalities detected on isolated fetal cells include trisomy 21, trisomy 18, Klinefelter syndrome (47,XXY) and 47,XYY. Polymerase chain reaction (PCR) technology has enabled the detection of fetal sex, Mendelian disorders (e.g. β-globin mutations), HLA polymorphisms, and fetal Rhesus (D) blood type. The fetal cell type that has generated the most success is the nucleated erythrocyte; however, trophoblasts, lymphocytes and granulocytes are also considered to be present in maternal blood. Fetal cells circulate in maternal blood during the first and second trimesters, and their detection is probably not affected by Rh or ABO maternal-fetal incompatibilities. Emphasis is now directed toward determining the most practical and efficacious manner for this technique to be applied to prenatal genetic diagnosis. Only upon completion of clinical evaluations could it be considered appropriate to offer this technology as an alternative to conventional invasive and non-invasive methods of prenatal cytogenetic diagnosis.