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Comparison of naphthalene bioavailability determined by whole-cell biosensing and availability determined by extraction with Tenax 总被引:1,自引:0,他引:1
Kohlmeier S Mancuso M Deepthike U Tecon R van der Meer JR Harms H Wells M 《Environmental pollution (Barking, Essex : 1987)》2008,156(3):803-808
A rapid biological method for the determination of the bioavailability of naphthalene was developed and its value as an alternative to extraction-based chemical approaches demonstrated. Genetically engineered whole-cell biosensors are used to determine bioavailable naphthalene and their responses compared with results from Tenax extraction and chemical analysis. Results show a 1:1 correlation between biosensor results and chemical analyses for naphthalene-contaminated model materials and sediments, but the biosensor assay is much faster. This work demonstrates that biosensor technology can perform as well as standard chemical methods, though with some advantages including the inherent biological relevance of the response, rapid response time, and potential for field deployment. A survey of results from this work and the literature shows that bioavailability under non-equilibrium conditions nonetheless correlates well with Koc or Kd. A rationale is provided wherein chemical resistance is speculated to be operative. 相似文献
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V. Somerset P. Baker E. Iwuoha 《Journal of environmental science and health. Part. B》2013,48(2):164-178
This paper reports the construction of the gold/mercaptobenzothiazole/polyaniline/acetylcholinesterase/polyvinylacetate (Au/ MBT/PANI/AChE/PVAc) thick-film biosensor for the determination of certain organophosphate pesticide solutions in selected aqueous organic solvent solutions. The Au/MBT/PANI/AChE/PVAc electrocatalytic biosensor device was constructed by encapsulating acetylcholinesterase (AChE) enzyme in the PANI polymer composite, followed by the coating of poly(vinyl acetate) (PVAc) on top to secure the biosensor film from disintegration in the organic solvents evaluated. The electroactive substrate called acetylthiocholine (ATCh) was employed to provide the movement of electrons in the amperometric biosensor. The voltammetric results have shown that the current shifts more anodically as the Au/MBT/PANI/AChE/PVAc biosensor responded to successive acetylthiocholine (ATCh) substrate addition under anaerobic conditions in 0.1 M phosphate buffer, KCl (pH 7.2) solution and aqueous organic solvent solutions. For the Au/MBT/PANI/AChE/PVAc biosensor, various performance and stability parameters were evaluated. These factors include the optimal enzyme loading, effect of pH, long-term stability of the biosensor, temperature stability of the biosensor, the effect of polar organic solvents, and the effect of non-polar organic solvents on the amperometric behavior of the biosensor. The biosensor was then applied to detect a series of 5 organophosphorous pesticides in aqueous organic solvents and the pesticides studied were parathion-methyl, malathion and chlorpyrifos. The results obtained have shown that the detection limit values for the individual pesticides were 1.332 nM (parathion-methyl), 0.189 nM (malathion), 0.018 nM (chlorpyrifos). 相似文献
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探讨了不同浓度Cu2+及Cu2+和Cd2+联合对活性污泥系统COD去除的影响,并采用ToxTell生物传感器,以活性污泥为指示微生物,分析了上述金属离子的毒性抑制作用.结果表明,Cu2+浓度低于10 mg.L-1时,不会对该活性污泥系统带来明显的冲击作用,Cd2+的加入明显增强了对该活性污泥系统COD去除的抑制作用,上述金属离子在抑制时间为1.5 h时对系统COD去除抑制率达到最大,随着曝气时间的延长,COD去除率呈逐渐上升趋势;ToxTell生物传感器的毒性分析结果与COD去除抑制率相近,初步证实该生物传感器能很好地用于活性污泥处理系统受Cu2+、Cd2+离子冲击的预警监测. 相似文献
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BOD生物传感器的研制与应用 总被引:1,自引:0,他引:1
介绍了BOD生物传感器的原理、研制过程以及实际测定。该仪器测定BOD的周期为30min,有较高的精密度和准确度,与稀释法有良好的相关性,可以及时信息反馈,特别适用于污水处理过程中的控制分析以及BOD的快速测定。 相似文献
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生物传感器在环境监测中的应用及发展前景 总被引:1,自引:0,他引:1
简单介绍了生物传感器的结构和基本原理、种类和特点,重点论述了生物传感器在水、大气及其他环境监测中的应用,展望了生物传感器未来的发展前景及发展方向。 相似文献
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生物传感器BOD快速测定仪的研究进展 总被引:1,自引:0,他引:1
生化需氧量(biochemical oxygen demand,BOD)作为表征水体中种类繁多的有机污染物的集合参数,在水质评价、污染监测以及废水处理厂的运行分析等方面发挥着重要作用.然而,传统的BOD测量需要5d,费时费力,不能及时反馈相关消息.因此,研究与开发方便、快速、准确的BOD测定仪受到了国内外学者的广泛关注.此文评述了BOD生物传感器快速测定仅的组成、性能、测定条件、响应速度等方面的国内外研究现状,分析了目前BOD传感器研究与开发中存在的问题和局限,并提出了今后进一步研究的方向. 相似文献