粘红酵母(Rhodotorula glutinis CIBAS A 1401)苯丙氨酸解氨酶cDNA核心序列的克隆与分析

L苯丙氨酸因其在工业、医药上的重要作用而日益受到人们的重视.苯丙氨酸解氨酶(PAL)生物转化肉桂酸生产L苯丙氨酸成了该领域研究与开发的热点.本文利用简并寡核苷酸PCR法结合cDNA末端快速扩增PCR法,从粘红酵母中克隆了苯丙氨酸解氨酶cDNA核心序列,为克隆其cDNA全长序列及体外表达其活性蛋白奠定了基础.图5参22     

Effects of nonylphenol on endocrine regulation-associated gene expression profiles in whole brain of F1 generation male rats

In order to determine the effects of nonylphenol (NP) on endocrine regulation-associated gene expression profiles in whole brain of F1 generation rats, mRNA extracted from the brain of 2-day old F1 generation rats whose F0 male generation was treated with NP. The mRNA was then reversely transcribed to cDNA and labeled with cy5 and cy3 fluorescence. Subsequently, cDNA probes were hybridized to the Mouse40S cDNA microarray and the fluorescent signals of cy5 and cy3 were scanned and analyzed. Sixteen genes were identified which expressed differently, including 13 that were down-regulated in which 4 were related to brain regulation of endocrine function. Data suggest that NP mainly affects metabolism and synthesis of steroid hormone in various ways and disturbs the reproductive function of male rats when administered perinatally.     

Effects of nonylphenol on the brain development-associated gene expression profiles of F1 generation rats

To explore the effects of nonylphenol on brain development-associated gene expression profiles of F1 generation rats by means of microarray technique. The mRNA were extracted respectively from the brain of 2-day-old F1 generation rats whose F0 male generation were tested with and without nonylphenol, respectively, then reversely transcribed to cDNA and labeled with cy5 and cy3 fluorescence. Subsequently,the cDNA probes were hybridized to the Mouse40S cDNA microarray and the fluorescent signals of cy5 and cy3 were scanned and analyzed. Sixteen identified genes expressed differently, including 13 down-regulated in which five were related to brain function and development. Nonylphenol can effect the brain function in many ways, mainly disturb metabolism, development and differentiation of neurocyte, and synthesis and release of neurotransmitter.