镉铅对小麦醇脱氢酶(ADH)基因表达影响的初步研究

经过镉，铅４５ｈ污染处理，运用Ｎｏｒｔｈｅｉｎ斑点杂分析和酶活性测定的方法探测萌发期小麦ＡＤＨ酶基因的表达情况。实验结果表明，ＡＤＨ基因表达在ＲＮＡ和酶活性两个层次均有变化，并且呈现相似的变化趋势，反映得金属的影响是来源于基因转录水平。     

单细胞凝胶电泳技术在环境污染物检测中的应用

主要介绍了单细胞凝胶电泳技术(SCGE),包括SCGE的原理、方法及其在环境污染物检测中的应用,该技术可用多种污染物的检测且灵敏度高,具有广阔的应用前景。即SCGE对大气污染物、重金属、醛类及辐射污染的检测,具有高度敏感性,是具有广阔应用前景的新技术。     

喷射浮选处理含油污水的研究

重点对喷射浮选处理含油污水进行了试验研究，结果表明浮选室内射流泵的没水深度对喷射选除油效果有较大的影响，并且只有在气泡量大并充满浮选室及表面相对平静的状态下，才儿得较好效果，为此，我们在浮选室内加入自制的增料，使兼并的气海重新得到分散，消耗了一部分动能，减缓了浮选室的扰动，使除在大提高，从而为研制新型高效的喷射浮选设备提供了一条新途径。     

Reduction of sera requirements in amniotic fluid cell culture

Reduction in serum requirement for culture of primary human amniotic fluid cells can be achieved by the addition of 10 growth-promoting factors to the nutrient medium. This supplemented medium preserves cell types normally found in amniotic fluid cell cultures supplemented with 20–30 per cent fetal bovine serum. The volume of amniotic fluid required to initiate culture can be as little as 1 ml. Amniotic fluid samples contaminated with red blood cells with no visible clot also grow well in the low serum medium. Cell-free amniotic fluid combined with equal parts of supplemented medium is useful in initiating cell culture.     

The effect of oxygen tension on colony formation and cell proliferation of amniotic fluid cellsin vitro

The effects of reduced oxygen concentrations in the gas phase over the culture medium on colony formation and cell proliferation were investigated in high and low cell density primary and secondary cultures of amniotic fluid cells. Using two standard culture methods (25 cm² plastic flasks and Leighton type tubes) a significantly reduced culture time was observed at high cell density for mass cultures by incubation within a low oxygen tension gas phase (2.5 per cent to 7.5 per cent O₂) instead of conventional air (18 per cent O₂). At low cell density colony formation was significantly enhanced in cultures grown at reduced oxygen tension. Using gas permeable membranes as support, lowering the oxygen tension from 7.5 per cent to 2.5 per cent yielded an increase in plating efficiency of cells from approximately 5 per cent to 25 per cent, whereas plating efficiency was less than 2 per cent for cells grown at ambient 18 per cent O₂. It is suggested that low oxygen tension in the gas phase is an effective means of enhancing clonal growth in amniotic fluid cell cultures, thereby reducing both culture time and risk of culture failure.     

镧、铈对厌氧颗粒污泥产甲烷的Hormesis效应及其动力学研究

间歇培养条件下研究了单一稀土元素镧(La3 )和铈(Ce3 )在不同浓度水平下对厌氧颗粒污泥活性及其动力学行为的影响.结果表明,La3 和Ce3 对厌氧颗粒污泥比产甲烷活性有促进作用的浓度范围均为0.01～0.1 mg·L-1,促进作用浓度峰值均为0.05mg·L-1,最大促进百分比分别为10.35%和20.79%;La3 和Ce3 对SMA(比甲烷活性)的半抑制浓度分别为5mg·L-1和1000mg·L-1,La3 较易对污泥产生抑制作用.米-门方程可以用来描述厌氧消化过程中甲烷发酵阶段的动力学行为,稀土元素La3 和Ce3 投加至反应系统后,提高了甲烷发酵过程的动力学常数Vmax和半饱和常数Ks.     

固定化酵母菌对活性艳蓝KN-R的脱色研究

采用海藻酸钙加活性碳的方法对酵母菌进行固定化。正交试验结果表明，三者合适配比为海藻酸钠3％，活性碳10％，氯化钙4％。此方法制得的凝胶颗粒机械强度好、经久耐用。固定化酵母菌凝胶小球最适条件为：培养时间为48h，培养温度为37℃，培养基pH为3．0，凝胶量为10g。固定化细胞经多次脱色后能重复使用，4次脱色后其脱色率还达79．2％。     

Characterisation of Organic Matter and Carbon Cycling in Rehabilitated Lignite-rich Mine Soils

Open-cast lignite mining in the Lusatian mining district resulted in rehabilitated mine soils containing up to four organic matter types: (1) recent plant litter, (2) lignite deposited by mining activity, (3) carbonaceous ash particles deposited during amelioration of the lignite-containing parent substrate and (4) airborne carbonaceous particles deposited during contamination. The influence of lignite-derived carbon types on the organic matter development and their role in the soil carbon cycle was unknown. This paper presents the findings obtained during a six year project concerning the impact of lignite on soil organic matter composition and the biogeochemical functioning of the ecosystem. The organic matter development after rehabilitation was followed in a chronosequence of rehabilitated mine soils afforested in 1966, 1981 and 1987. A differentiation of the organic matter types and an evaluation of their role within the ecosystem was achieved by the use of ¹⁴C activity measurements, ¹³C CPMAS NMR spectroscopy and wet chemical analysis of plant litter compounds. The results showed that the amount and degree of decomposition of the recent organic matter derived from plant material of the 30 year old mine soil was similar to natural uncontaminated forest soil which suggests complete rehabilitation of the ecosystem. The decomposition and humification processes were not influenced by the presence of lignite. On the other hand it was shown that lignite, which was thought to be recalcitrant because of its chemical structure, was part of the carbon cycle in these soils. This demonstrates the need to elucidate further the stabilisation mechanisms of organic matter in soils.     

显微构造分析法和包裹体方法在断层研究中的应用

在工程建设中，工地及邻近地区的断层活动性是工程设计的一项重要依据。在总结以往研究的基础上，介绍了显微构造分析法和包裹体方法在断层活动性评价中的国内外最新研究成果。事实表明，这两种方法在工程地质领域具有良好的应用前景。     

1995年世界重要地震灾情及地震活动特征

1995年全世界发生197次6级以上地震，其中有30次7级以上的地震（最大震级8．1级）。具有灾害性的地震为33次，伤亡20人以上的，国内及边界地区为4次，国外有15次，其中有2次灾难性的大地震。该年地震与1994年相比呈明显增强趋势，为本世纪以来第三个强震最多之年。1995年世界地震灾害的主要特点为地震袭击现代化大城市，造成惨重的人员伤亡，惊人的财产损失数额。