Extraction of DNA from amniotic fluid cells for the early prenatal diagnosis of genetic disease

Ten-ml samples of amniotic fluid were taken from pregnancies being terminated at 8–14 weeks' gestation. DNA was extracted from the amniotic cells by sequential centrifugation and analysed using the polymerase chain reaction (PCR). Fifteen samples were analysed for evidence of maternal contamination using Mfd5 oligo-nucleotide primers for repeat polymorphisms. Ten amniotic fluid samples were tested for the Delta-F₅₀₈ deletion characteristic of cystic fibrosis to demonstrate a diagnostic application for the technique. In each case, DNA extracted from fetal tissue from the same pregnancy was included in the controls. In 14 of the 15 cases tested with the Mfd5 primers, both the amniotic fluid DNA and the fetal DNA showed no evidence of contaminating DNA. In one case, neither the amniotic fluid cells nor the fetal cells yielded results. In nine of the ten cases tested with the Delta-F₅₀₈ primers, the amniotic fluid cell DNA provided accurate information about the genetic status of the fetus; in the tenth, the fetal DNA failed to amplify. The results indicate that adequate DNA can be extracted from amniotic fluid from 8 weeks' gestation onward and these samples are suitable for prenatal diagnosis using PCR.     

S-100 protein and neuron-specific enolase in amniotic fluid as markers of abdominal wall and neural tube defects in the fetus

The aim of this study was to determine whether there is increased leakage of neuron-specific enolase (NSE) and S-100 protein into amniotic fluid in pregnancies with neural tube defects, since both these proteins are produced by neural tissue, and to compare the value of these substances for detecting such defects with that of the more conventional techniques of alpha-fetoprotein (AFP) and acetylcholinesterase (AChE) gel electrophoresis. Amniotic samples from 25 mid-pregnancies (15–17 weeks' gestation) with neural tube defects (14 with open spina bifida and 11 with anencephaly) and from seven mid-pregnancies with abdominal wall defects were compared with a control material consisting of 80 amniotic fluid samples from 80 consecutive mid-pregnancy amniocenteses, with normal karyotypes and AFP concentrations. All of the above cases of abnormalities were primarily detected through increased AFP levels in the amniotic fluid. Amniotic fluid samples from 13 pregnancies with fetuses with autosomal chromosomal abnormalities and seven amniotic fluid samples contaminated with blood were also included in the investigation. It is concluded from the results that the conventional AFP assay combined with AChE gel electrophoresis is the best method for screening amniotic fluid for neural tube defects and defects of the abdominal wall. Neither NSE nor S-100 assay alone proved to be superior for the detection of these cases in mid-trimester amniotic fluid. The S-100 assay, however, could give additional information in cases where AChE gel electrophoresis is not decisive; for example, in samples contaminated with blood.     

21世纪中国环境法制建设的理性回归

本文从近几年我国环境法制建设状况的统计入手，详细地分析了近年我国环境问题的特点和环境法制建设的特点。但是，环境问题在我国仍然非常严峻，我国的环境法制建设仍然有诸多的缺陷，而且新世纪我国社会经济发展呈现出了新的特点。基于此，文章从六个方面对新世纪我国的环境法制建设进行了冷静地分析与思考，并因此得出相应的结论。     

广西南部三叠纪强过铝质火成岩岩石化学特征的动力学意义

为深化对广西南部地区印支期造山运动深部动力学机制的理解,采用该地区三叠纪强过铝质火成岩的岩石化学数据提取其源区熔融温度以及成分特征方面的信息。结果显示:广西南部三叠纪强过铝质火成岩属于高钾钙碱性系列,形成于印支期后碰撞构造背景中。它们的Al2O3/TiO2小于100,CaO/Na2O多数大于0 3、少数小于0 3。同时,这些强过铝质火成岩的Al2O3/TiO2与其SiO2含量有正相关趋势,而CaO/Na2O与SiO2含量呈明显的负相关关系。这些岩石化学特征表明,强过铝质火成岩部分源于泥质岩的部分熔融,部分源于砂岩或正变质岩的部分熔融,但不排除后者可能是泥质岩部分熔融产物和玄武质岩浆相混合的结果;同时这些强过铝质火成岩形成源区的温度高(>=875℃)。因此,广西南部三叠纪时期的深部能量来自以底侵玄武岩浆加热作用所体现出来的软流圈地幔的平流热传递过程。     

水质模拟及趋势分析

根据洛河、伊河河流及流经地区特征 ,选择具有代表性的断面 ,选用一维河流有限差分水质模型 ,计算洛河、伊河 1 995～2 0 0 0年主要断面污染物浓度 ,并对其进行评价。采用季节性肯达尔检验法 ,对计算出的洛河、伊河 1 995～ 2 0 0 0年主要断面污染物浓度进行趋势分析 ,并分析其变化特征及形成原因     

浙江安吉地区地质灾害及其防治对策

本文介绍了安吉地区地质灾害的调查概况，对其成因类型，时空分布，与地质环境的关系和防治对策作了探讨，这圣方政府编制地质灾害防治规划具参考意义。     

利用EXCEL求算水中非离子氨

Excel是办公自动化中非常重要的一款软件，它的数据处理功能在现有的文字处理软件中可以说是独占螯头，通过简单的学习，结合国家标准给出的换算公式，利用EXCEL编制程序，方便快捷地计算水中非离子氨浓度，计算结果表明，当实际测得水温和pH与表中数据吻合时三种计算方法结果无明显差异，当实际测得的水温和pH与表中不对应时，查表法存在明显偏差，而使用EXCEL运算就能与笔算结果一致，准确可靠。     

矩形截面毛坯挤压矩形工件的上限研究

应用上限法建立了由矩形毛坯正挤压矩形截面工件的等挤压比流动模型 ,推导出了三次多项式表示的流线方程 ,给出了变形区的动可容速度场、应变速率场及上限功率的表达式 ,获得了挤压力的上限数值解 ;分析了变形程度、工件截面形状等参数对挤压力的影响 ,并获得了相对挤压应力与截面形状系数之间的近似关系式。研究证明采用流线速度场进行上限分析比其他方法更符合实际情况 ,根据流线设计的凹模优于常规凹模 ,如平底模。     

Sustainable development pattern and the strategy in the three Gorges Reservoir Areas

The development pattern,development situation,and existing problems of land exploitation in Zigui County,Three Gorges Reservoir Areas of China were presented.The sustainable development mode and its strategy in the Three Gorges Reservoir Areas was also discussed.A sustainable development framework for low mountain regions,middle mountain regions and high mountain regions was developed,and management countermeasures for structural optimization of complex ecosystems were advanced.     

城市空气质量中长期预报的探讨

江苏省环境空气质量预报工作已开展多年,在这几年的预报工作中,发现现在使用的对未来24 h空气质量预报的方式上存在一些问题,从而限制空气质量预报发挥应有的作用.改进预报方式,在现有的空气质量预报的基础上,开展城市空气质量的中长期预报,有利于各级政府及环境管理部门,提前采取必要的措施,减少或控制污染的产生,从而避免城市空气污染事件的发生,以保护城市居民的身体健康.