四环素抗生素对污泥中四环素抗性基因丰度和表达水平的作用影响

为研究四环素(TC)抗生素对其抗性基因(TC-ARGs)转录表达的作用影响,以从活性污泥中筛选获得的四环素抗性细菌(TRB)作为研究对象,采用荧光定量PCR和逆转录PCR方法检测了7种TC-ARGs,包括tet A、tet C、tet G、tet M、tet O、tet W和tet X基因的丰度和表达水平,并探讨了TC与TC-ARGs丰度及其表达水平之间的相关关系.结果表明,在整个培养周期内,tet A、tet G和tet W基因丰度随TC暴露浓度的增大总体呈现上升趋势,但其余TC-ARGs基因丰度整体波动较大. TC胁迫对不同TC-ARGs的转录表达水平作用影响差别较大,其中,tet A基因表达水平相对稳定,且随TC浓度的升高而上调,在TC浓度为100 mg·L^-1时,其上调倍数高达5. 3倍.在短期(1 d) TC胁迫下,TC-ARGs转录表达水平随TC浓度的升高整体呈现上调趋势.由相关性分析可知,tet A和tet W基因丰度与其转录表达水平之间存在显著的线性相关性,表明其基因丰度可在一定程度上衡量和评价其抗性表达水平,进而反映其功能活性和环境风险.     

基于Sentinel-2 MSI影像的河湖系统水体悬浮物空间分异遥感监测:以安徽省升金湖与连接长江段为例

开展河湖系统悬浮物监测对掌握水体泥沙运移规律、制定水环境治理措施具有重要意义.以安徽省升金湖与连接长江段水体为研究区,根据实测光谱模拟Sentinel-2 MSI影像波段反射率,结合同步水体悬浮物实测数据建立反演模型;而后根据2017～2019年28景MSI影像水体悬浮物反演结果,分析河湖系统水体悬浮物浓度的变化规律,并探究水位变化对其空间分异的影响.结果表明:①根据MSI影像第六波段与第三波段的比值建立的二次多项式模型具有较高的反演精度(R²=0.863,RMSE=22.211 mg·L^-1),适用于高浊度水体悬浮物反演;②在空间上,升金湖入湖口附近、上中湖区西北部和下湖悬浮物浓度相对较高,除夏季外升金湖悬浮物浓度均高于其连接长江段;在时间上,升金湖悬浮物浓度在夏季相对较低,在其他季节较高,而与其连接的长江水体呈现相反的年内变化规律;③闸控影响下河湖连通性改变造成的水位变化,是影响升金湖-长江悬浮物空间分异的关键因素.在平水期与枯水期,升金湖对长江悬浮物浓度变化具有一定的贡献度,而在丰水期,升金湖与长江悬浮物浓度变化之间的相关性不明显.     

Development of inherent safety and health index for formulated product design

The numerous formulated products which are introduced to the market consist of chemical ingredients that may cause various safety and health hazards to the consumers. Therefore, it is extremely important to practice a systematic methodology to formulate products with acceptable safety and health performances. This work presents an index-based methodology to assess the safety and health hazards of the ingredients during the early formulation stage of product design. Hence, new inherent safety and health sub-indexes are introduced to improve the current safety and health hazards that are needed in formulated product design. The inherent safety and health sub-indexes are assigned with scores based on the degree of potential hazards. A higher score indicates a higher safety risk or severe health effect, and vice versa. The proposed methodology will greatly assist the users to identify the adverse safety and health effects caused by the ingredients. Hence, it is pivotal to eliminate or reduce the safety and health impacts from product usage. A case study on common ingredients used in the formulation of paint is presented on this study to describe the proposed method.     

区域优势自然资源的评价——以河南省黄河两岸地区为例

本文试图从区域、自然资源本身和资源的外部条件出发,通过分析区域优势自然资源的概念,选择建立了衡量区域优势自然资源的6项标准体系,并以河南省黄河两岸地区为例,借助与高层次区域的比较研究,确定了该区域的优势自然资源是:铝土矿为第一优势资源,天然气为第二优势资源,以下资源次序为石油、煤、金矿、水泥灰岩、耐火粘土和水资源等。这为合理开发区域优势自然资源提供了重要科学依据.     

ESTIMATING CENTRAL TENDENCY FROM UNCENSORED TRACE LEVEL MEASUREMENTS1

ABSTRACT: The statistical analysis of data which have trace level measurements has traditionally been a two-step process in which data are first censored using criteria based on measurement precision, and then analyzed with statistical methods for censored data. The process might be more informative if data were left uncensored. In this paper, information loss attributable to censoring and measurement noise are assessed by comparing the sample mean and median of uncensored measurements with a log regression mean and median based on censored data. Measurements are derived from lognormal parent distributions which have random variability characteristic of trace level measurement. The relative performance of estimators used with error-free samples and with samples having measurement noise can be explained by differences between the probability distributions of parents and measurements. Measurement introduces bias and dispersion and transforms lognormal parent distributions toward greater symmetry. Estimates using uncensored data are less biased and more accurate than the log regression mean and median when censoring exceeds about 50 percent, and are not much worse at any fraction censored. For data with many (80 percent) results below the limit of detection, bias may be quite severe.     

济南泉域地下水补给区保护分级及核心保护区承载力分析

近年来由于地下水补给区的盲目开发和市区地下水超采，素以“泉城”闻名的济南市泉水持续断流，加强泉域地下水补给区的生态环境保护是解决这一问题的关键，在详细研究区域地质水文条件的基础上借助地理信息系统，将补给区划分为三级保护区，即核心保护区，直接补给区和间接补给区，并应用环境承载力分析的理论方法，确定出保护区23个汇水区的环境承载人口，本文对泉域地下水补给区总体发展战略和具体保护规划的制定具有重要的意义。     

卤虫对毒物毒性监测方法研究

以孵化好的 - 期卤虫为受试生物 ,实验研究了 As2 O3、Hg Cl2 、KCN、K2 Cr2 O7、苯、苯酚、六六六7种物质对卤虫的中毒阀值和 L C50 - 2 4h(L eathal、Concentration 5 0 - 2 4hours,2 4小时半致死浓度 )的测定 ,该方法具有操作简便、快速、覆盖面宽、技术易掌握、所需设备不复杂等特点。     

两个相邻高建筑物周围的风环境

描述研究了建筑物周围人行道高度风速分布特征的风洞模拟实验。结果表明：在一个横风向宽度很大的高大建筑物的迎风面拐角附近，风速增大约１倍；而在建筑物的前方有风速的骤变区，这均形成严重的风环境问题。当用一通道将该建筑物分割成两个相邻建筑物时，上述两方面的风环境问题得到了有效的改善，且通道宽度与建筑物顺风向尺度之比的优化数值在１８至２４之间     

三门峡水利枢纽地震形变监测中库水位干扰的剔除方法

本文通过对三门峡水利枢纽工程外部地震形变监测成果的分析，阐述了大坝地震形变监测的基本内容，简述了三门峡水利枢纽及其与库区水位相关的变化规律，提出了解决水利枢纽地震安全监测中剔除库水位干扰的通用方法。     

基于经济空间分异的扬州市生态镇建设模式研究

扬州市乡镇经济发展程度空间分异明显。应用主成分分析法分析了扬州市88个乡镇经济水平的空间差异,指出经济发达、欠发达乡镇的分布,整体上呈现南强北弱的态势。进而提出不同经济水平的生态镇建设模式,即:经济发达乡镇主导发展生态工业;欠发达乡镇巩固农业优势,主导发展特色资源型生态农业和观光农业。