Characterization of microbial communities from coastal waters using microarrays

Molecular methods, including DNA probes, were used to identify and enumerate pathogenic Vibrio species in the Chesapeake Bay; our data indicated that Vibrio vulnificus exhibits seasonal fluctuations in number. Our work included a characterization of total microbial communities from the Bay; development of microarrays that identify and quantify the diversity of those communities; and observation of temporal changes in those communities. To identify members of the microbial community, we amplified the 16S rDNA gene from community DNA isolated from a biofilm sample collected from the Chesapeake Bay in February, 2000. The resultant 75 sequences were 95% or more similar to 7 species including two recently described Shewanella species, baltica and frigidimarina, that have not been previously isolated from the Chesapeake. When the genera of bacteria from biofilm after culturing are compared to those detected by subcloning amplified 16S fragments from community DNA, the cultured sample exhibited a strong bias. In oysters collected in February, the most common bacteria were previously unknown. Based on our 16S findings, we are developing microarrays to detect these and other microbial species in these estuarine communities. The microarrays will detect each species using four distinct loci, with the multiple loci serving as an internal control. The accuracy of the microarray will be measured using sentinel species such as Aeromonas species, Escherichia coli, and Vibrio vulnificus. Using microarrays, it should be possible to determine the annual fluctuations of bacterial species (culturable and non-culturable, pathogenic and non-pathogenic). The data may be applied to understanding patterns of environmental change; assessing the health of the Bay; and evaluating the risk of human illness associated with exposure to and ingestion of water and shellfish.     

西藏公路建设过程中的生态保护

针对西藏独特的高原生态环境特征，分析了公路建设的生态影响，提出了不同生态系统类型的生态恢复和保护措施。     

乙酸型顶极群落的内平衡与反馈调节机制研究

通过产酸脱硫反应器处理高浓度硫酸盐有机废水的连续流试验，从“动态”角度考察COD／SO4^2-比改变引起的pH值、氧化还原电位（ORP）、碱度（ALK）和末端产物（VFAs）等的变动及生态因子的叠加效应引发的优势种群变迁，分析了乙酸型顶极群落的稳定性及其发生定向性生态演替的规律，进而阐明了乙酸型顶极群落抵抗环境压力的内平衡与反馈调节机制，并指出乙酸型代谢和乙酸型顶极群落是产酸脱硫生态系统的典型特征。     

Effect of free-air CO2 enrichment on nematode communities in a Chinese farmland ecosystem

At a rice-wheat rotational free-air CO2 enrichment(FACE) platform, the effect of elevated atmospheric CO2 on soil nematode communities in a farmland ecosystem was studied. Wheat plots were exposed to elevated atmospheric CO2 (ambient 370 μl/L 200 μl/L).32 families and 40 genera of nematode were observed in soil suspensions during the study period. Under FACE treatment, the numbers of total nematodes, bacterivores and fungivores exhibited an increasing trend. Because of the seasonal variation of soil temperature and moisture, the effect of elevated atmospheric CO2 on soil nematodes was only observed under favorable conditions. The response of nematode communities to elevated atmospheric CO2 may indicate the change of soil food web.     

面向未来的企业可持续发展必由之路:生态效益

介绍工业生态学中生态效益的基本概念、核心思想和企业向生态效益型发展的必要性和必然性 ,分析了推行生态效益管理的关键和难点 ,并提出我国企业推行生态效益的对策建议。     

构建云南省新型生态工业园区的思考

论述了构建云南新型生态工业园区的必要性,对构建云南生态工业园提出了设想和初步设计,并分析了存在问题,提出了保证措施。     

织物干洗的工业生态研究

正确评价干洗剂对人体的毒副作用，合理地设定干洗过程挥发性有害物质的残留限量，是开发干洗业环境无害技术和绿色服务的先决条件。只有在充分保证环境安全的前提下，通过逐步推行化学清洁产品的总体选择战略，从环境管理和经济发展的结合上来规范企业的环境行为，才能最终实现干洗业的可持续发展。     

略论中国绿色社区建设

绿色社区建设在中国刚刚起步 ,这是一种全新的发展理念、发展目标和发展模式 ,是实施可持续发展战略的现实要求 ,是加快中国城市化进程的必然选择 ,是全面建设小康社会的重要内涵 ,是创造绿色文明的热切呼唤。建设绿色社区具有重要的理论指导意义和实践示范作用。     

产酸脱硫反应器中COD/SO_4~(2-)比制约的群落生态演替规律

通过产酸脱硫反应器的动态试验和配套的静态试验 ,考察致变因子COD/SO2 - 4比制约的乙酸型顶极群落的结构、优势种群的组成和生态演替的规律 ;阐明乙酸型代谢和乙酸型顶极群落是产酸脱硫生态系统的典型特征 ;揭示乙酸型顶极群落内平衡与反馈调节的生理代谢机制 ,并以因变因子 pH值、氧化还原电位和碱度来表征生态演替过程中优势种群的三维实现生态位