两类藻毒素聚酮合成酶遗传相似性及其二级结构预测分析

研究微囊藻毒素聚酮合成酶、节球藻毒素聚酮合成酶之间的遗传关联性,并对其二级结构进行预测分析.应用聚合酶链反应得到2株蓝绿藻的毒素聚酮合成酶(PKS)基因,并进行基因序列分析.从GenBank中提取产微囊藻毒素、产节球藻毒素藻株的相应基因序列,利用DNAStar和phylip软件分析目的基因一致性及2类藻毒素PKS的进化情况.采用Garnier-Robson法、Karplus-Schulz法预测项圈藻株202A1/35、节球藻株NSOR10PKS蛋白片段的二级结构,Kyte-doolittle法分析蛋白的亲水性,Emini法预测蛋白质的表面可能性.结果表明,2类藻毒素PKS目的基因的相似性非常高;项圈藻属、念珠藻属的微囊藻毒素PKS与节球藻毒素PKS的进化关系较近;2种藻毒素PKS分析片段二级结构具有较大的相似性,其亲水性与表面可能性区域等特征也极为相似.     

受火约束钢梁在升温段和降温段行为的理论分析(Ⅰ)

真实火灾中的调查和试验结果表明,约束钢梁比一般的无约束钢梁有更好的抗火承载性能。在梁端约束作用下,火灾中的约束钢梁能够产生很大的变形而且不会在瞬间坍塌。在梁中产生的轴力对梁在火灾中的行为有很大影响。影响约束钢梁行为的因素包括:荷载类型、轴向约束刚度、转动约束刚度、梁截面温度分布等。根据梁端约束情况和荷载类型,对梁的变形曲线进行了假设,据此可得出梁的轴力以及梁截面内应变与应力的分布。通过对应力的积分,可得出截面轴力及抵抗弯矩,然后结合梁的平衡方程,就可求出梁在火灾下的变形。为了进行火灾后的安全评估及修复工作,还需要了解钢梁在降温段的反应。当温度降低时,钢材的强度会得到恢复,这对恢复梁的承载能力是有利的,但是同时由于梁的收缩,梁内将进一步产生拉力,这对梁及两端的节点是很不利的。本文对约束钢梁在降温段的行为进行了比较深入的理论研究,并推荐了一种简单的计算方法。     

CFG桩复合地基加固高速公路软基的现场试验研究

CFG桩复合地基处理高速公路软基的设计参数是否合理,应看其实际发挥的承载能力及承载时变形的性状。通过对CFG桩复合地基桩、土应力和表面沉降的现场观测,研究了路堤荷载下CFG桩复合地基桩顶、桩间土的应力和沉降变化规律,根据实测数据分析了褥垫层厚度、桩间距及桩体强度等设计参数的合理性。结果表明,路堤荷载下,CFG桩、土最终可达到变形协调,桩、土应力比与桩、土沉降差有着密切的关系,疏桩形式时桩间土承担着大部分荷载;同时,CFG桩复合地基作为路堤荷载的地基时,可设计为桩间距较大的疏桩形式,桩体设计强度可以取得低一些,褥垫层厚度也应适当取大。     

隧道竖井外流场模拟及风塔外型结构优化研究

为提高长大隧道施工及运营通风过程中竖井通风效率,实现隧道节能通风。依托实际隧道工程,基于Fluent软件,选用RNG k-ε湍流模型,对无竖井风塔、圆柱风塔、矩形风塔和凸台状风塔4种结构、不同环境风速工况下的流场进行数值模拟,并对模拟结果进行交叉比对分析,提出最优化的竖井风塔结构形式。研究结果表明:采用风塔结构竖井出流量要远大于无风塔结构竖井出流量;对于不同竖井风塔外型结构,竖井出流量与环境风速关系呈多项式函数;当倾斜角为45°、圆心角为90°时,凸台结构对应竖井出流量最大,且受环境风影响波动较小,通风稳定性较好。建议竖井出口结构选用矩形或凸台状,实现对环境风的高效利用,提高竖井通风效率。     

基于RFPA2D复合局部挠曲岩体数值模拟

为研究复合局部挠曲岩体强度特征以及破坏规律,采用岩石真实破裂软件RFPA^2D,对水平层面挠曲30°,45°,60°,75°的复合岩体进行单轴压缩试验模拟。模拟结果表明:挠曲角为30°,45°,60°,75°的复合岩体破坏面几乎与挠曲段夹层重合,其挠曲端部均产生了垂直于挠曲段夹层的裂纹;复合单斜岩体与复合挠曲岩体破坏面的形成因素大致相同,夹层强度是2种岩体失稳的主要因素;复合挠曲岩体单轴抗压强度随挠曲角增大同样呈“U”型变化,与单斜岩体变化趋势一致;当水平层状岩体发生挠曲后,其单轴抗压强度减小,当挠曲角为60°时,强度降低25.19%,当挠曲角为75°时,强度降低0.17%。;随着均质系数m的增大,复合挠曲岩体单轴抗压强度以及轴向应变均出现逐渐递增的趋势,且不同m值,其岩体裂隙扩展方式具有明显差别。     

鄱阳湖平原区农村门塘浮游藻类群落特征及其生物学评价

于2018年—2019年在鄱阳湖区周边选取4个县（区）10个村庄的典型门塘开展浮游藻类采样调查。研究共鉴定出藻类6门83种,以蓝藻门、绿藻门和硅藻门为主;浮游藻类细胞密度全年范围为7.30×104个/L～2.78×1011个/L,年均值为1.4×1010个/L,其中夏季细胞密度最大,冬季细胞密度最小;优势种主要有小环藻、微囊藻、铜绿微囊藻、卵形隐藻等,且具有较为明显的季节演替,全年优势种为小环藻;藻类多样性指数（H′）年均值为1.49,丰富度指数（M）年均值为1.92,全年水体生物学评价结果为中度污染。     

A bibliometric study of pool fire related publications

Pool fire is a common form of fire, which is constantly investigated along with the development of fire science and is also comprehensively employed as stable fire sources in examining other fire scenarios such as building and tunnel fires. According to the records in Science Citation Index Expanded database in the Web of Science Core Collection, a total of 1073 articles or reviews related to pool fires have been published from 1966 to 2019. In order to have a better understanding of knowledge structure of this topic and further identify its development history and currently popular concerns, a bibliometric analysis of pool fire research is conducted by means of visualization software VOSviewer and CiteSpace. This work visually provides a comprehensive overview of pool fire research in terms of annual publication output, source journals, productive countries/regions, authors and their cooperation network, subject terms, and reference co-citation analysis. The analysis provides networks of co-cited references, authors, countries, subject terms, and their respective clusters, indicating their ranking in contributions to the pool fire related publications. The results can be applied to enhance the understanding of pool fire research and support further work in this area.     

单芯铜线过电流故障电弧熔痕的微观特征研究*

为准确认定电气火灾中过电流故障,模拟4~7倍额定电流（Ie）条件下单芯铜导线过电流故障,利用光学显微镜和扫描电子显微镜（SEM）分析过电流故障电弧熔痕的组织特征,并结合Image-Pro Plus软件测定晶粒直径、周长和面积,探究金相组织的量化判据和变化规律。结果表明:当I＝4Ie时,过电流故障电弧熔痕的金相组织主要为方向性较强的树枝晶;当I＝5Ie,6Ie时,金相组织内树枝晶占比减小,胞状晶占比升高;当I＝7Ie时,方向性较弱的胞状晶占主导。随着电流增加,晶粒的平均直径、周长和面积均呈增长趋势。通过火灾案例分析可知,实验结果与现场的电弧熔痕组织特征基本相符,验证火灾事故原因为过电流故障。     

电气柜疲劳强度校核及结构优化

针对多孔闭孔型发泡硅橡胶D型空心密封条的超弹性特性,通过试验找出了密封条回弹力与压缩量之间的关系,提出了密封条满足IP X5的判别方程式,同时对方程式进行了试验验证,最后对密封条进行了阿累尼乌斯图寿命推算,为变流器柜门IP防护设计提供了重要参考。     

Rainfall Interception Based on Indirect Methods: A Case Study in Temperate Forests in Oaxaca,Mexico

Rainfall interception represents the amount of water trapped in natural cover that is not drained directly to the ground. Intercepted rainfall may evaporate after a rain event, making it one of the main drivers of water balance and hydrologic regionalization. This process can be affected by factors such as climate, altitude, vegetation type, and topography. Here is a simple method of calculating rainfall interception in temperate forests using in Santa Maria Yavesia, Oaxaca, and Mexico as an illustrative study area. We used two rain gauges to measure net precipitation (N_p) under the canopy at each study site and one gauge outside the canopy to obtain gross precipitation (G_p). Throughfall (T_h) was indirectly measured using hemispherical photographs. Rainfall interception was obtained through a combination T_h and G_p and N_p. The mean rainfall interception was 50.6% in the Abies forests, 23%–40% in the coniferous‐mixed forests, and 27.4% in the broad‐leaved forests. We classified rainfall events by intensity to determine the effect of canopy structure and precipitation and found that 75% of the events were weak events, 24% were moderate events, and 1% were strong events. In addition, we found that rainfall interception was lower when the intensity of precipitation was higher. Our method can be replicated in different ecosystems worldwide as a tool for assessing the influence of rainfall interception in terms of ecological services.