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231.
奎屯市城市污水的处理现阶段采用一级处理后接生物稳定塘的处理工艺是较为适宜的,但要注意做好污水及处理水的排放监测,加强排水管理,今后要逐步完善为规范的二级处理工艺。对污水处理要制定切实可行的水污染防治规划,不拘泥于现有的排水方案和污水处理厂选址,在污水厂下游建设规范的污水库作为处理水资源化利用的必要措施。我区多数城市水污染防治路线与奎屯市有着相似性,对各城市污水处理方式及利用途径可采用此方法进行深入细致的研究论证,为未来城市污水的有效处理及无害化、资源化利用打下基础。 相似文献
232.
233.
Schröder P Scheer CE Diekmann F Stampfl A 《Environmental science and pollution research international》2007,14(2):114-122
Background, Aim and Scope
Numerous herbicides and xenobiotic organic pollutants are detoxified in plants to glutathione conjugates. Following this enzyme
catalyzed reaction, xenobiotic GS-conjugates are thought to be compartmentalized in the vacuole of plant cells. In the present
study, evidence is presented for long range transport of these conjugates in plants, rather than storage in the vacuole. To
our knowledge this is the first report about the unidirectional long range transport of xenobiotic conjugates in plants and
the exudation of a glutathione conjugate from the root tips. This could mean that plants possess an excretion system for unwanted
compounds which give them similar advantages as animals.
Materials and Methods:
Barley plants (Hordeum vulgare L. cv. Cherie) were grown in Petri dishes soaked with tap water in the greenhouse.
- Fluorescence Microscopy. Monobromo- and Monochlorobimane, two model xenobiotics that are conjugated rapidly in plant cells
with glutathione, hereby forming fluorescent metabolites, were used as markers for our experiments. Their transport in the
root could be followed sensitively with very good temporal and spatial resolution. Roots of barley seedlings were cut under
water and the end at which xenobiotics were applied was fixed in an aperture with a thin latex foil and transferred into a
drop of water on a cover slide. The cover slide was fixed in a measuring chamber on the stage of an inverse fluorescence microscope
(Zeiss Axiovert 100).
- Spectrometric enzyme assay. Glutathione S-transferase (GST) activity was determined in the protein extracts following established
methods. Aliquots of the enzyme extract were incubated with 1-chloro-2,4-dinitrobenzene (CDNB), or monochlorobimane. Controls
lacking enzyme or GSH were measured.
- Pitman chamber experiments. Ten days old barley plants or detached roots were inserted into special incubation chambers, either
complete with tips or decapitated, as well as 10 days old barley plants without root tips. Compartment A was filled with a
transport medium and GSH conjugate or L-cysteine conjugate. Compartments B and C contained sugar free media. Samples were
taken from the root tip containing compartment C and the amount of conjugate transported was determined spectro-photometrically.
Results:
The transport in roots is unidirectional towards the root tips and leads to exsudation of the conjugates at rates between
20 and 200 nmol min-1. The microscopic studies have been complemented by transport studies in small root chambers and spectroscopic
quantification of dinitrobenzene-conjugates. The latter experiments confirm the microscopic studies. Furthermore it was shown
that glutathione conjugates are transported at higher rates than cysteine conjugates, despite of their higher molecular weights.
This observation points to the existence of glutathione specific carriers and a specific role of glutathione in the root.
Discussion:
It can be assumed that long distance transport of glutathione conjugates within the plant proceeds like GSH or amino acid
transport in both, phloem and xylem. The high velocity of this translocation of the GS-X is indicative of an active transport.
For free glutathione, a rapid transport-system is essential because an accumulation of GSH in the root tip inhibits further
uptake of sulfur. Taking into account that all described MRP transporters and also the GSH plasmalemma ATPases have side activities
for glutathione derivatives and conjugates, co-transport of these xenobiotic metabolites seems credible.
- On the other hand, when GS-B was applied to the root tips from the outside, no significant uptake was observed. Thus it can
be concluded that only those conjugates can be transported in the xylem which are formed inside the root apex. Having left
the root once, there seems to be no return into the root vessels, probably because of a lack of inward directed transporters.
Conclusions:
Plants seem to possess the capability to store glutathione conjugates in the vacuole, but under certain conditions, these
metabolites might also undergo long range transport, predominantly into the plant root. The transport seems dependent on specific
carriers and is unidirectional, this means that xenobiotic conjugates from the rhizosphere are not taken up again. The exudation
of xenobiotic metabolites offers an opportunity to avoid the accumulation of such compounds in the plant.
Recommendations and Perspectives:
The role of glutathione and glutathione related metabolites in the rhizosphere has not been studied in any detail, and only
scattered data are available on interactions between the plant root and rhizosphere bacteria that encounter such conjugates.
The final fate of these compounds in the root zone has also not been addressed so far. It will be interesting to study effects
of the exuded metabolites on the biology of rhizosphere bacteria and fungi. 相似文献
234.
Analysis of the nitrifying bacterial community in BioCube sponge media using fluorescent in situ hybridization (FISH) and microelectrodes 总被引:2,自引:0,他引:2
There is growing interest in the development of more cost-effective and retrofit technologies for the upgrade and expansion of existing wastewater treatment plants with extreme space constraints. A free-floating sponge media (BioCube) process, using a 24L lab scale reactor, was operated to study the nitrification profiles and microbial community. The COD removal efficiencies were maintained, at an average of 95%, with the mixed liquor suspended solids (MLSS) inside the BioCube sponge media maintained at 12,688mg/L. The nitrification removal efficiencies were between 92% and 100%, with an average value of 99%. From the results of microelectrode measurements, the ammonium ion concentration was found to rapidly decrease from the surface of the BioCube sponge media to a depth of 2mm due to chemical reactions carried out by ammonia oxidizing bacteria (AOB) species. Multi-fluorescence in situ hybridization (FISH) has been used to investigate the spatial distributions of various microbial activities within reactors. Microbial communities were targeted using different oligonucleotide probes specific to AOB and nitrite oxidizing bacteria (NOB). There were a large number of AOB populations, but these were not uniformly distributed in the biofilm compared to the NOB populations. 相似文献
235.
采用体外受精技术评价环境污染物 2 ,2’ ,4,4’ 和 3,3’ ,4,4’ 四氯联苯对小白鼠卵母细胞受精能力和 2 细胞胚胎发育的毒性作用 .实验结果表明 :ρ(PCB) =0 .1,1.0 ,10 .0mg/mL的 2 ,2’ ,4,4’ 和 3,3’ ,4,4’ 四氯联苯均显著降低小白鼠卵母细胞的受精能力 ;ρ(PCB) =1.0 ,10 .0mg/mL的 2 ,2’ ,4,4’ 和 3,3’ ,4,4’ 四氯联苯使卵母细胞的退化率和 2 细胞的畸形率显著增加 ;两种四氯联苯对小白鼠卵母细胞体外受精能力、退化率和 2 细胞胚胎的畸形率无统计差异 .表 2参 2 5 相似文献
236.
237.
黄河下游历史泥沙灾害的宏观特征及其与流域因素和人类活动的关系(Ⅱ):—人类活动、历史地震及地形因子的影响 总被引:2,自引:1,他引:1
以历史上黄河下游的决溢频率为泥沙灾害的代用指标,研究了人类活动,历史地震及地形因子对黄河下游历史泥沙灾害的影响,研究表最,人类活动是影响黄河下游泥沙灾害的重要因素,历史上人口的增加使下游决溢频率增大,人口低谷与决溢频率的低值时段相应,历史上农牧交错带的南移与下游决溢频率的减小相伴发生。北移则导致决溢频率的增大,历史地震对黄河下游历史泥沙灾害有一定的影响,黄河下游历史泥沙灾害表现出明显的空间分异,这种分异与平原地貌条件的沿程变化有密切的关系。 相似文献
238.
结构分析中模拟与仿真技术研究 总被引:5,自引:1,他引:4
由于像洪水、水灾、地震这样一类自然灾害的原型重复试验几乎是不可能的,因此计算机仿真技术在这一领域的应用具有重要的意义。本文探索了土木工程中模拟与仿真的概念、特征及实现途径,阐述了关于仿真智能化、可视化的技术细节及仿真的基本思路;结合面向对象程序设计(OOP)概念,提出了结构分析程序设计的原则及标准。在此基础上,编写了基于Windows的结构有限元分析软件SmartSEA. 相似文献
239.
孙文科 《防灾减灾工程学报》2001,21(2):30-33
以某大楼的基底反力监测为例 ,阐述了建筑物基底反力实时监测的原理与方法。监测分析结果表明 ,对在建建筑物进行的基底反力实时监测直接反映了土体受力状态 ,可间接得出桩基的受力情况。 相似文献
240.
姚大全 《防灾减灾工程学报》2001,21(2):15-18
对安徽省殷家汇葛公镇断裂旁侧方解石脉进行了野外考察和显微观测。露头上可识别出多期变形事件 ,显微镜下也能鉴别出脉的不同期次的变形特征和变形强度 ,脉的变形强度表现为从早期到晚期 ,脉的变形形态和组构从复杂趋于简单 ,方解石机械双晶从多组、弯曲趋于单组、平直。压性或压剪性脉体变形以交错揉皱为其主要特征 ,而张性、张剪性脉体则多表现为破裂、砾化等脆性变形形态。殷家汇葛公镇断裂宏观和微观系统分析研究为基岩区断裂活动期次及各期变形特征的鉴别研究提供了一个实例。 相似文献