Biofilm-overproducing Bacillus subtilis B12ΔYwcc decreases Cd uptake in Chinese cabbage through increasing Cd-immobilizing related gene abundance and root surface colonization

Biofilm-producing bacteria can decrease Cd uptake in vegetables, but mechanisms underlying this effect are poorly characterized. In this study, two mutant strains B12ΔYwcc and B12ΔSlrR were constructed from a biofilm-producing Bacillus subtilis strain B12. Then, the impacts of strain B12 and its high biofilm-producing mutant strain B12ΔYwcc and low biofilm-producing mutant strain B12ΔSlrR on Cd availability and uptake in Chinese cabbage and the related mechanisms were investigated in the Cd-polluted soil. Strain B12 and its mutants B12ΔYwcc and B12ΔSlrR increased the dry biomasses of edible tissues by 54%–130% compared with the controls. Strain B12 and its mutant B12ΔYwcc reduced the soil available Cd content by 36%–50% and root and edible tissue Cd contents by 23%–50% compared with the controls. Furthermore, the mutant strain B12ΔYwcc reduced the edible tissue Cd content by 40% and increased the polysaccharide content by 23%, invertase activity by 139%, and gene copies of the cumA by 4.5-fold, epsA by 7.1-fold, and cadA by 4.3-fold, which were involved in Cd adsorption in the rhizosphere soils, respectively, compared with strain B12. The polysaccharide content and cumA, epsA, and cadA gene copy numbers showed significantly reverse correlations with the available Cd content. Notably, the mutant strain B12ΔYwcc showed better ability to colonize the vegetable root surface than strain B12. These findings demonstrated that the biofilm-overproducing mutant strain B12ΔYwcc increased the polysaccharide production and Cd-immobilizing related cumA, epsA, and cadA gene copies, resulting in lower Cd availability and accumulation in Chinese cabbage in the Cd-polluted soil.     

WTO规则对西部开发的影响、风险及机遇浅析

随着中国加入WTO和西部大开发战略的实施 ,依法治国无疑是改善投资环境、促进西部开发的最有效手段之一。本文根据WTO基本原则与法制经济的相互关系和影响深入浅出地论述了WTO对西部开发的影响、风险及机遇 ,并提出了针对性的策略     

高职院校实验室资源优化配置与科学管理简

实验室资源优化配置和科学管理是高职院校提高教学、科研水平和人才培养质量的重要途径。当前高职院校实验室资源配置与管理中普遍存在实验室分散封闭管理、资源配置缺乏计划、实验室没有充分利用以及设备缺乏维护保养四个方面问题,针对这些问题应从管理体制改革、设备申报采购制度、实验室潜力开发以及开展培训、提高管理人员水平四个方面来实现实验室合理配置资源、科学规范管理。     

荧光法快速测定浮游植物以及应用于梅梁湖预警监测可行性探讨

YSI 6600-V2多功能水质自动监测仪采用荧光原理快速测定水体中浮游植物蓝绿藻的密度,梅梁湖夏季水华高发期时,湖体中的浮游植物几乎全部是蓝绿藻。传统的浮游植物监测前处理方面就需要24~48 h,最终要数天后才有镜检结果,导致浮游植物数据滞后,不能很好的应用到预警监测中。通过比对相关性验证,保证荧光法数据的可信度,提高工作效率,为预警监测提供有力的支持。     

Effect of Monitoring Technique on Quality of Conservation Science

Monitoring free‐ranging animals in their natural habitat is a keystone of ecosystem conservation and increasingly important in the context of current rates of loss of biological diversity. Data collected from individuals of endangered species inform conservation policies. Conservation professionals assume that these data are reliable—that the animals from whom data are collected are representative of the species in their physiology, ecology, and behavior and of the populations from which they are drawn. In the last few decades, there has been an enthusiastic adoption of invasive techniques for gathering ecological and conservation data. Although these can provide impressive quantities of data, and apparent insights into animal ranges and distributions, there is increasing evidence that these techniques can result in animal welfare problems, through the wide‐ranging physiological effects of acute and chronic stress and through direct or indirect injuries or compromised movement. Much less commonly, however, do conservation scientists consider the issue of how these effects may alter the behavior of individuals to the extent that the data they collect could be unreliable. The emerging literature on the immediate and longer‐term effects of capture and handling indicate it can no longer be assumed that a wild animal's survival of the process implies the safety of the procedure, that the procedure is ethical, or the scientific validity of the resulting data. I argue that conservation professionals should routinely assess study populations for negative effects of their monitoring techniques and adopt noninvasive approaches for best outcomes not only for the animals, but also for conservation science. Efecto de la Técnica de Monitoreo en la Calidad de la Ciencia de la Conservación     

Interactions Among the Copper Species and Forms in Sea Water/Sediments and Copper Bioaccumulation in Oysters

Abstract

Earlier papers indicated that the first incident of green discoloration in oysters (Crassostrea gigas) and the mass mortality observed in 1986 along the Taiwan Erhjin Chi coastal area were caused by the higher contents of total copper and copper species (mainly bioavailable and free ion) in sea water. the copper in sea water would be sorbed by suspended matter and transferred to sediments, and the copper in the sediments would also be desorbed to sea water. Processes of copper adsorption and desorption are the major factors influencing the contents of total copper and copper species in sea water and sediments. in this study, the Erhjin Chi sediments were mixed with sea water by a shaker technique. When the mixture was shaken for one hour, analogous to tidal mixing in estuaries, only copper desorption from sediments was observed. If the shaking time is increased for more than 3 hours, the copper released from the sediments was resorbed to the remaining solid phases. the higher the contents of mud (91.71%) and total copper (701 mg kg^?1) in sediments, the higher the copper desorption rate (1.86 ppm hr^?1) and copper adsorption rate (0.50 ppm hr^?1) were observed. in sediments containing lower mud (0.80%) and lower copper (43.5 mg kg^?1), the copper desorption and adsorption rates were 0.83 ppm hr^?1 and 0.22 ppm hr^?1, respectively. the interactions among the total copper and copper species in sea water and sediments, chemical and ecological parameters, and copper bioaccumulation in oysters in the Erhjin Chi estuarine and coastal area are also discussed.     

采用间隔流动注射仪测定总氰和氰化物的探讨

采用间隔流动注射的方法对水质中的氰化物进行检测。通过选择合适的锌盐和关闭的紫外灯,将总氰模块应用于易释放氰化物的检测。结果表明,仪器测定的总氰和易释放氰化物的精密度、准确度和加标回收率均满足水质检测的质量控制要求。     

汶川地震灾区受灾群众重建意愿实证研究

选择何种重建方式(原址重建或异地搬迁等)是灾后重建的首要问题.所谓重建意愿即在原居住地受到地震等自然灾害破坏时,受灾居民对不同重建方式(原址重建还是异地搬迁或者两者之间的状态)的选择倾向.了解灾区群众的重建意愿及其因素影响是受灾县(市)制订灾后重建规划、并推动实施的重要前提.通过对五大极重灾区1243户城乡受灾居民家庭问卷调查、深度访谈.结果表明:受灾居民所在县(市)、目前安置地、户口类型、受教育程度、适应新环境能力及未来经济预期等因素对其重建意愿有显著影响.研究认为:选择重建方式应在尊重群众意愿的前提下,结合实际充分论证;在此基础上,应结合目前的临时安置地对城乡布局合理规划;应将灾后重建和城乡统筹及生态扶贫相结合来考虑,着力提高受灾居民的就业技能和受教育程度,从而提高适应新环境生产生活的能力;加大在灾区的产业恢复重建力度,特别尽快恢复关系民生的产业以及就业容量大的产业.     

Detoxification of ferrocyanide in asoil–plant system

The detoxification of iron cyanide in a soil–plant system was investigated to assess the total cyanide extracted from contaminated soil and allocated in the leaf tissue of willow trees(Salix caprea). They were grown in soil containing up to 1000 mg/kg dry weight(dw) of cyanide(CN),added as ~(15)N-labeled potassium ferrocyanide and prepared with a new method for synthesis of labeled iron cyanides. CN content and ~(15)N enrichment were monitored weekly over the exposure in leaf tissue of different age. The ~(15)N enrichment in the young and old leaf tissue reached up to 15.197‰ and 9063‰, respectively; it increased significantly over the exposure and with increasing exposure concentrations(p 0.05). Although the CN accumulation in the old leaf tissue was higher, compared to the young leaf tissue(p 0.05), the ~(15)N enrichment in the two tissue types did not differ statistically. This indicates a non-uniform CN accumulation but a uniform ~(15)N allocation throughout the leaf mass. Significant differences were detected between the measured CN content and the C~(15)N content, calculated from the ~(15)N enrichment(p 0.05), revealing a significant CN fraction within the leaf tissue, which could not be detected as ionic CN. The application of labeled iron CN clearly shows that CN is detoxified during uptake by the willows. However, these results do not exclude other detoxification pathways, not related to the trees. Still, they are strongly indicative of the central role the trees played in CN removal and detoxification under the experimental conditions.