深水型水库热分层诱导水质及真菌种群结构垂向演替

水体真菌种群在调控水库生态系统健康过程中发挥重要作用,为探明热分层诱导深水型水库水体真菌种群结构垂向演替特征,采集金盆水库主库区0.5、10、25、40、60和70 m水深水样,分析水质参数、并运用高通量DNA测序技术诊断水库水体真菌种群结构垂向异质性.结果表明,金盆水库表层水温最高为22.33℃,底层最低为7.21℃(P0.05).水体溶解氧(DO)随水深增加显著降低(P0.05),电导率、总磷(TP)和铁含量随着水深增加显著升高(P0.05),垂向形成稳定的变温层、斜温层和等温层结构.水体真菌种群高通量DNA测序共发现1 247个OTUs,隶属4门14纲39属,主要包括接合菌门(Zygomycota)、担子菌门(Basidiomycota)、子囊菌门(Ascomycota)和壶菌门(Chytridiomycota).等温层水体真菌种群Shannon指数和Chao 1多样性指数最高,分别为3.45和360,显著高于斜温层水体(P0.05).红酵母属(Rhodotorula,27.23%)、链格孢属(Alternaria,24.28%)、分子孢子菌属(Cladosporium,22.98%)、链格孢属(Alternaria,32.00%)、亚隔孢壳属(Didymella,17.47%)和分子孢子菌属(Cladosporium,28.17%)分别是0.5、10、25、40、60、70 m的优势菌属,不同水深均存在大量未知真菌(Unclassified).热图(heat map)结果表明,热分层期间金盆水库水体真菌种群结构垂向分布差异显著、不同水层具有不同的真菌种群结构.主成分分析(PCA)表明水温、DO、TP、电导率是调控水体真菌种群结构垂向异质性的主要水质因子.该研究结果为水源水库热分层期间水质与真菌群落的偶联机制提供科学依据.     

二氧化硫胁迫下拟南芥miRNA表达谱分析

以拟南芥为材料,利用高通量测序技术并结合生物信息学分析方法,检测二氧化硫(SO_2)处理后拟南芥植株的小分子RNA表达谱,筛选SO_2胁迫响应microRNAs(miRNAs)分子,研究植物miRNAs对逆境胁迫的应答机制.结果发现,30 mg·m~(-3) SO_2处理72 h后,拟南芥地上组织小分子RNA长度分布发生改变,在对照组和SO_2组中均有大量特有的小分子RNA序列,说明SO_2胁迫可诱导拟南芥小分子RNA的表达改变.SO_2胁迫诱导186个保守miRNA和16个新miRNA分子差异表达,其靶基因主要涉及转录调控、信号转导、代谢、刺激响应等生理过程.差异表达的miR160和miR393可通过生长素信号途径调控植株生长发育,参与植物对SO_2的胁迫响应.本研究揭示了植物中参与SO_2胁迫应答的miRNA种类及作用机制,进一步阐明了miRNAs在植物抗逆应答过程中的作用.     

RELATIONSHIP OF NITRATE CONCENTRATIONS TO DISTANCE OF WELL SCREEN OPENINGS BELOW CASING WATER LEVELS1

ABSTRACT: Data from 54 well in central and eastern Kansas developed in unconsolidated deposits of Quaternary age indicate that nitrate concentrations are inversely related to the depth of the well screen opening below the water level in the well casing because this relationship was found to exist in an area of Nebraska and in a large area of Kansas, the relationship is generally valid over a wide geo- graphical area. In addition, the data indicate that the incidence of nitrate concentrations exceeding 45 milligrams per liter and nitrate concentrations, in general, are significantly lower in water from wells with screens deeper than 25 feet below the water table in unconfined aquifers or where screens are placed in deep confined aquifers. No concentrations of nitrate greater than 45 milligrams per liter were in obseved wells where screens were deeper than 60 feet below the casing water level. These findings suggest that general placement of well screens as far below the water table as possible in unconfined unconsolidated aquifers in Kansas. and possibly other areas of the Midwest, may be an effective measure in preventing undesirable nitrate concentrations in ground water supplies. particularly in areas where nitrate is a problem.     

Preimplantation genetic diagnosis using FISH for carriers of Robertsonian translocations: the Portuguese experience

Preimplantation genetic diagnosis (PGD) is an alternative to prenatal diagnosis for couples at risk of transmitting genetic disorders to their offspring. We present a fluorescence in situ hybridization (FISH) analysis of embryos obtained after seven PGD cycles in six couples with Robertsonian translocations and male factor infertility: 4 der(13;14), 1 der(14;21) and 1 der(15;21). Of 74 metaphase II (MII) injected oocytes, 61 (82.4%) fertilized normally and cleaved. Of these, 37/61 (60.7%) embryos were of high morphological quality with ≥6 blastomeres. After biopsy of 44 embryos at day 3 of development, seven degenerated, seven arrested in development and 30/44 (68.2%) evolved, of which 25/30 (83.3%) reached the morula/blastocyst stage. Analysis of biopsied blastomeres showed 23/44 (52.3%) of normal/balanced embryos, of which 15 (11 at the morula/blastocyst stage) were transferred in six cycles. One term pregnancy was achieved, which ended by cesarean section at 37 weeks of gestation, giving birth to two healthy newborn. Analysis of 49 embryos (excluding 12 inconclusive cases) showed a predominance of alternate segregation (38/49, 77.6%) over adjacent segregation (7/49, 14.3%), with one (2%) being a polyploid mosaic and three (6.1%) chaotic. Copyright © 2002 John Wiley & Sons, Ltd.     

STREAM DEPLETION BY WELLS IN THE SOUTH PLATTE BASIN-COLORADO1

The agricultural production from the Lower South Platte Basin in Colorado represents a significant portion of the state economy. Until the early 1950's the production had developed almost exclusively by use of river water. Drought conditions combined with improved well technology resulted in an inordinate amount of well development in the valley during the period 1952-56. These wells were used for supplemental supply in many cases, but the application of sprinkler irrigation brought many acres of here-to-fore dry land into irrigated production. As a result of the vast amounts of groundwater withdrawal by the newly developed wells, senior surface appropriators found a decreasing amount of water available for use in the streams. The legislature, observing the doctrine of prior appropriation, ruled that all surface and ground water in a tributary would be treated and administered as one resource. This, of course, spelled doom for the well-oriented segment of the economy. Analysis of a segment of the river on an inflow-outflow basis was made with careful determination of all inflow-outflow in the study reach to include correlations required to determine ungaged side-channel in-flow and unmetered irrigation wells. Results indicate that wells have intercepted normal return flows to the river resulting in a decreased amount of surface water during the irrigation season. Stream depletion appears to equal the expected consumptive use of well water which ranged between 40% to 50% of the groundwater extraction.     

The many benefits of employee well‐being in organizational research

We undertake three objectives in this Incubator. We begin with a discussion of how well‐being has been considered in organizational research. Second, we provide an overview of the relationship between employee well‐being and such workplace outcomes as job performance, employee retention, and cardiovascular health. Third, we introduce exciting research directions. Copyright © 2012 John Wiley & Sons, Ltd.     

湿法脱硫系统优化分析

分析了运用烟塔合一等技术对现有湿法脱硫系统简化的可行性.在湿法脱硫系统中,利用冷却塔排烟气代替烟囱排烟,取消烟囱和GGH换热器,将增压风机和引风机合二为一,可提高能源的利用效率,减少设备的投资,降低系统运行及维护费用.     

西安地铁车站深基坑变形规律FLAC模拟研究

开展西安地铁深基坑变形规律理论与监测的研究对指导西北地区深基坑信息化施工具有重要价值。本文以西安地铁2号线某车站深基坑工程为背景,完成了车站深基坑施工监测方案设计,对深基坑施工过程进行了FLAC计算模拟,重点研究了桩体变形、钢支撑轴力、基坑周边地表变形规律。结果表明,复杂环境下城市地铁车站站深基坑明挖施工时,现场监测是信息化安全施工的保证,采用钻孔灌注桩和钢支撑的复合围护方案作为车站深基坑的围护结构是合理的,土方分层开挖方式和钢支撑预应力施加是减少空间效应保证安全施工的重要措施。桩身水平位移特别是桩顶水平位移是围护结构变形特性的直接反映,围护桩变形最大的地方为基坑中部到三分之二基坑深度处。基坑围护结构附近的地面隆起量明显小于基坑中部的隆起量,随着开挖深度增大,隆起量逐渐由基坑中部最大转变为两边大中间小的型式。     

深井开采巷旁充填沿空留巷围岩活动规律研究

随着我国煤矿开采深度的逐年增加,瓦斯含量逐渐增大且赋存异常,回采工作面瓦斯超限问题严重威胁着煤矿的安全生产,而沿空留巷则是解决工作面瓦斯超限问题的最有效途径,但沿空留巷顶板稳定性控制问题则制约了沿空留巷技术在我国更广泛地推广应用。本文以顾桥矿1115(1)采煤工作面沿空留巷的成功案例为背景,全面研究分析了沿空留巷围岩活动的基本规律,对类似条件下的沿空留巷围岩控制具有重要的指导意义,使沿空留巷技术在我国得到更广泛的推广应用具有积极的现实意义。     

深孔预裂爆破对含坚硬顶板高瓦斯煤层的综合作用研究

为研究深孔预裂爆破技术在含坚硬顶板高瓦斯煤层开采中的应用,以顾桥矿1123(1)工作面坚硬顶板为研究对象,基于小挠度理论建立了坚硬顶板垮落力学模型,导出了初次来压步距计算式;综合考虑瓦斯抽采需要,对深孔预裂爆破孔进行了优化设计,并在工作面顶板中开展了现场试验研究。结果表明:在工作面推进至27.3m时出现垮落,爆破后钻孔瓦斯流量显著增加,部分提高至30倍以上,瓦斯抽采量提高了3.5倍,初采期间安全回采煤炭25万吨,实现了坚硬顶板控制和卸压增透双重作用。