Extraction of DNA from amniotic fluid cells for the early prenatal diagnosis of genetic disease

Ten-ml samples of amniotic fluid were taken from pregnancies being terminated at 8–14 weeks' gestation. DNA was extracted from the amniotic cells by sequential centrifugation and analysed using the polymerase chain reaction (PCR). Fifteen samples were analysed for evidence of maternal contamination using Mfd5 oligo-nucleotide primers for repeat polymorphisms. Ten amniotic fluid samples were tested for the Delta-F₅₀₈ deletion characteristic of cystic fibrosis to demonstrate a diagnostic application for the technique. In each case, DNA extracted from fetal tissue from the same pregnancy was included in the controls. In 14 of the 15 cases tested with the Mfd5 primers, both the amniotic fluid DNA and the fetal DNA showed no evidence of contaminating DNA. In one case, neither the amniotic fluid cells nor the fetal cells yielded results. In nine of the ten cases tested with the Delta-F₅₀₈ primers, the amniotic fluid cell DNA provided accurate information about the genetic status of the fetus; in the tenth, the fetal DNA failed to amplify. The results indicate that adequate DNA can be extracted from amniotic fluid from 8 weeks' gestation onward and these samples are suitable for prenatal diagnosis using PCR.     

导流明沟人工湿地治理橡胶废水实践

运用人工湿地原理，采用明沟导流治理橡胶生产废水，其工程投资低，占地面积小，运行稳定，易于管理，去除率高，处理后废水达到国家标准。     

水质模拟及趋势分析

根据洛河、伊河河流及流经地区特征 ,选择具有代表性的断面 ,选用一维河流有限差分水质模型 ,计算洛河、伊河 1 995～2 0 0 0年主要断面污染物浓度 ,并对其进行评价。采用季节性肯达尔检验法 ,对计算出的洛河、伊河 1 995～ 2 0 0 0年主要断面污染物浓度进行趋势分析 ,并分析其变化特征及形成原因     

青岛市建设循环型经济社会的探讨

以青岛市为例,通过对传统型经济社会发展所面临环境问题的对比分析,阐述了发展循环经济、建设循环型经济社会对我国经济社会可持续发展的重要意义。指出为了实现2008年“绿色奥运”和生态型城市的建设目标,在青岛市发展循环经济,建立循环型经济社会的可能性和必要性。提出了青岛市发展循环经济、建设循环型经济社会的实施对策。     

Spatial distribution of ecological security status assessment of West-Liaohe River based on geographic information system

Eco-security assessment is a hot research area in resource and environmental science, which involves data with much spatial, non-linear, and random features. Geographic information system (GIS), as a useful tool to analyze and manage spatial information, has a superior advantage in this field. A case study in the western part of the Liaohe River featuring a method of eco-security spatial differences (ESSD) based on GIS is developed in this paper. The method includes four steps: 1) developing the pressure-state-response (P-S-R) framework with site data; 2) digitizing West-Liaohe River and setting its GRID database of ecosecurity assessment indicators; 3) figuring out the relative membership degree (RMD) of eco-security indicators by using the analytical hierarchy process with the weight of indicator; 4) classifying the security zone and mapping the assessment result of eco-security status in grid by GIS method of assigning and clustering. The visual spatial differences of eco-security based on GIS enables decision makers to know the status of eco-security better in making policies for achieving sustainability. __________ Translated from Environmental Science, 2005, 26(5): 28–33 [译自: 环境科学]     

调引太湖水改善苏州市水动力条件研究

基于苏州地区独特的地理位置,简述引水改善该地区水环境的必要性,拟定了3种调水方案,利用感湖河网数学模型,对3种方案调水后主要河道的水动力条件进行预测,分析了各河道调水后的水动力变化趋势,提出了利用太湖水自流引水改善苏州市水环境的最佳方案.     

大庆红岗生态工业园区创建的现有条件

生态工业园是根据循环经济理念和工业生态学原理而设计建立的一种新型工业组织形态,在园区内通过废物交换、循环利用、清洁生产等手段,实现资源利用的最大化和废物排放的最小化.文章通过对大庆市石油、石油化工产业链现状分析及产品代谢和废物代谢分析,说明大庆红岗具有创建生态工业园区的区位和资源优势,园区所在区域内现已经初步形成了石油开采、石油化工、轻烃加工和化工建材产业(品)代谢连及废油、废水回注等废物代谢链的生态工业雏形,为创建大庆红岗生态工业(石化)示范园区奠定了一定的基础.     

论违反《中华人民共和国固体废物污染环境防治法》的刑事责任

系统分析《中华人民共和国固体废物污染环境防治法》所规定的三种犯罪类型,论述追究固体废物污染环境罪刑事责任的犯罪构成要件。     

嘉定城区河流水质现状评价及污染防治对策

通过对嘉定中心城区河流的水质现状调查和评价，提出了引清排污、加强市政措施等污染防治对策。     

黄海北部对虾放流水域污染分析

根据１９９６－１９９８年对黄海北部中国虾放流增殖水域的调查，对该水域的污染状 评价，结果表明，除个别站位ＣＯＤ、Ｃｕ、Ｚｎ、Ｈｇ有少量超标外，综合评价和单因子评价均符合渔业水质标准规定的要求，水质良好，适合幼对虾的栖息和生长。但污染物含量特别是重金属含量有逐年增高的趋势。