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101.
Hae Sang Jun Bong Oh Kim Yeon Chul Kim Ho Nam Chang Seong Ihl Woo 《Journal of Polymers and the Environment》1994,2(1):9-18
Copolyesters containing poly(ethylene terephthalate) (PET) and poly(-caprolactone) (PCL) were synthesized from PET and PCL homopolymers by transesterification reaction at 270°C in the presence of catalyst. The copolyesters were characterized by13C-NMR and differential scanning calorimetry (DSC). The degradation behavior of PCL byPseudomonas sp. lipase in buffer solution (pH 7) and tetrahydrofuran (THF) was investigated by gel permeation chromatography (GPC) and1H-NMR. From these experiments, it was found thatPseudomonas sp. lipase acted endoenzymatically on PCL. Using this lipase, degradation tests for PET/PCL copolyesters whose PCL content was below 50% by weight were also performed in buffer solution (pH 7). However, evenPseudomonas sp. lipase with high degradation activity on PCL did not easily degrade the PCL unit in PET/PCL copolyesters. 相似文献
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Ana PICADO Susana M. PAIXÃ O Liliana MOITA Luis SILVA Má rio S. DINIZ Joana LOURENÇ O Isabel PERES Luisa CASTRO José Brito CORREIA Joana PEREIRA Isabel FERREIRA Antó nio Pedro Alves MATOS Pedro BARQUINHA Elsa MENDONCA 《Frontiers of Environmental Science & Engineering》2015,9(5):793
The new properties of engineered nanoparticles drive the need for new knowledge on the safety, fate, behavior and biologic effects of these particles on organisms and ecosystems. Titanium dioxide nanoparticles have been used extensively for a wide range of applications, e.g, self-cleaning surface coatings, solar cells, water treatment agents, topical sunscreens. Within this scenario increased environmental exposure can be expected but data on the ecotoxicological evaluation of nanoparticles are still scarce. The main purpose of this work was the evaluation of effects of TiO2 nanoparticles in several organisms, covering different trophic levels, using a battery of aquatic assays. Using fish as a vertebrate model organism tissue histological and ultrastructural observations and the stress enzyme activity were also studied. TiO2 nanoparticles (Aeroxide® P25), two phase composition of anatase (65%) and rutile (35%) with an average particle size value of 27.6±11 nm were used. Results on the EC50 for the tested aquatic organisms showed toxicity for the bacteria, the algae and the crustacean, being the algae the most sensitive tested organism. The aquatic plant Lemna minor showed no effect on growth. The fish Carassius auratus showed no effect on a 21 day survival test, though at a biochemical level the cytosolic Glutathione-S-Transferase total activity, in intestines, showed a general significant decrease (p<0.05) after 14 days of exposure for all tested concentrations. The presence of TiO2 nanoparticles aggregates were observed in the intestine lumen but their internalization by intestine cells could not be confirmed. 相似文献
104.
In‐vivo enzymatic activity tests have been used for the characterization of sediments. Sediment samples representing three typical situations on the river Elbe and one reference site on the river Spree were investigated from March to October 1993. Fresh sediments and pore water were analyzed for their dehydrogenase and proteolytic enzymatic activities, respectively. The dehydrogenase and protease activities varied depending on sampling site, date and kind of sample. From the mean values of the dehydrogenase activities over the whole period of investigation significant differences between the sampling sites became evident. The dehydrogenase activity of pore water rather than that of the whole sediment is suitable to differentiate between anthropogenically influenced and not influenced sites. The dehydrogenase activities in the contaminated samples were extremly higher than those in the uncontaminated samples. In contrast to this, samples from the uncontaminated sites showed a considerably higher specific proteolytic activity. This fact is interpreted as a possible enzymatic inhibition. 相似文献
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研究地沟油的脱胶工艺,比较了磷酸脱胶、水脱胶和磷酸-水联合脱胶3种方法的脱胶效果,考察了脱胶温度、磷酸用量、酸化时间、水用量和水化时间对磷酸-水联合脱胶效果的影响,并通过正交试验对其脱胶条件进行了优化。结果表明,磷酸脱胶、水脱胶和磷酸-水联合脱胶的脱胶率分别为8.78%、68.18%和79.32%;磷酸-水联合脱胶的最优脱胶条件为:脱胶温度60℃,磷酸用量0.1%,酸化时间40 min,水用量3%,水化时间30 min,在此条件下,脱胶率为96.70%。因此,磷酸-水联合脱胶可除去地沟油中的胶体,达到生物柴油原料的要求。 相似文献
108.
Dynamic changes in microbial activity and community structure during biodegradation of petroleum compounds: A laboratory experiment 总被引:5,自引:0,他引:5
With 110-d incubation experiment in laboratory,the responses of microbial quantity,soil enzymatic activity,and bacterial community structure to different amounts of diesel fuel amendments were studied to reveal whether certain biological and biochemical characteristics could serve as reliable indicators of petroleum hydrocarbon contamination in meadow-brown soil,and use these indicators to evaluate the actual ecological impacts of 50-year petroleum-refining wastewater irrigation on soil function in Sbenfu irrigation area.Results showed that amendments of≤1000 mg/kg diesel fuel stimulated the growth of aerobic beterotrophic bacteria,and increased the activity of soil dehydrogenase,hydrogenperoxidase,polypbenol oxidase and substrate-induced respiration.Soil bacterial diversity decreased slightly during the first 15 d of incubation and recovered to the control level on day 30.The significant decrease of the colony forming units of soil actinomyces and filamentous fungi can be taken as the sensitive biological indicators of petroleum contamination when soil was amended with≥5000 mg/kg diesel fuel.The sharp decrease in urease activity was recommended as the most sensitive biochemical indicator of heavy diesel fuel contamination.The shifts in community structure to a community documented by Sphingomonadaceae withinα-subgroup of Proteobacteria could be served as a sensitive and precise indicator of diesel fuel contamination.Based on the results described in this paper,the soil function in Shenfu irrigation area was disturbed to some extent. 相似文献
109.
A strain WL-11 with high laccase activity was isolated from activated sludge collected from the effluent treatment plant of a textile and dyeing industry.It was identified as Aeromonas hydrophila by physiological test and 16S rDNA sequence analysis.A gene encoding of laccase from a newly isolated Aeromonas hydrophila WL-11 was cloned and characterized.Nucleotide sequence analysis showed an open reading frame of 1605 bp encoding a polypeptide comprised of 534 amino acids.The primary structure of the enzyme predicted the structural features characteristic of other laccases,including the conserved regions of four histidine-rich copper-binding sites.The predicted amino acid sequence showed a high homology(more than 60%) with bacterial laccases in the genome and protein databases and the highest degree of similarity(61% identity) was observed with the multicopper oxidase of Klebsiella sp.601.When expressed in Escherichia coli,the recombinant enzyme was overproduced in the cytoplasm as soluble and active form.The purified enzyme had an optimum pH of 2.6 and 8.0 for ABTS(2,2'-azino-bis(3-ethylbenzthiazolinesulfonic acid) and DMP(2,6-dimethoxyphenol),respectively.The kinetic study on ABTS revealed a higher affinity of this enzyme to this substrate than DMP. 相似文献
110.
Cd对小白菜生长及氮素代谢的影响研究 总被引:7,自引:0,他引:7
采用水培的方法,研究了不同Cd2 水平(0、1、2.5、5、10 mg·L-1)对小白菜叶片中铵态氮、硝态氮、可溶性蛋白质、游离脯氨酸、叶绿素、部分营养元素含量以及蛋白水解酶、硝酸还原酶、谷氨酰胺转化酶与合成酶活性的影响.结果表明,低浓度的Cd处理(1 mg·L-1)刺激了小白菜的生长,提高了小白菜的生物量、叶绿素含量以及硝酸还原酶、谷氨酰胺合成酶与转化酶活性.Cd处理降低了小白菜对Cu、Ca、Fe、Mg的吸收,但促进了P的吸收.10 mg·L-1的Cd处理显著降低了可溶性蛋白质含量、硝酸还原酶、谷氨酰胺合成酶和转化酶活性(p<0.05),提高了蛋白水解酶活性,不利于叶片中铵态氮与硝态氮的同化,造成叶片中铵态氮和硝态氮的累积.小白菜叶片中游离脯氨酸含量与铵态氮含量成极显著正相关(p<0.01),说明小白菜叶片中游离脯氨酸的累积在一定程度上缓解了铵的毒害. 相似文献