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排序方式: 共有241条查询结果,搜索用时 390 毫秒
181.
Yi Tao Xianzhong Mao Jiangyong Hu H.O.L. Mok Lingyun Wang D.W.T. Au Jia Zhu Xihui Zhang 《Chemosphere》2013
This study aims to investigate the effects of UV-C irradiation on photosynthetic processes of Microcystis aeruginosa to unravel the mechanism(s) involved in how and in what ways UV-C mediates growth suppression and cellular recovery. Changes in the concentration of photosynthetic pigments, photochemical efficiency, PS II core protein (D1) content, and the coding genes expressions were measured. The results indicate that UV-C doses at 20–200 mJ cm−2 lead to rapid reduction in gene expression of both psbA (for D1) and cpc (for phycocyanin), but the suppression was short term and recoverable within 3 d of post-UV incubation. Conversely, UV-C doses at ?50 mJ cm−2 could induce marked decline in photochemical efficiency (represented by the optimal PS II quantum yield, FV/FM, and the effective PS II quantum yield, Y) as well as decreases in D1 content and water soluble pigments (phycoerythrins, phycocyanins, allophycocyanins) in M. aeruginosa during the post UV-C incubation period. The results suggest that interruption of both the light energy harvesting apparatus (especially the water soluble pigments) and the photochemical process mainly accounted for the growth suppression effect in UV-C irradiated M. aeruginosa. 相似文献
182.
对26根受火后的钢管陶粒混凝土短柱进行了轴压力学性能试验,试件考虑了钢管的几何参数、陶粒混凝土配合比及火灾条件等因素的变化。基于试验结果,分析了轻骨料钢管混凝土短柱受火后的轴压承载力、破坏形态及其他力学性能的变化及相关参数的影响规律。所讨论的主要影响参数包括试验最高炉温、最高炉温持续时间、钢管长细比、混凝土配合比等,并基于对试验数据的分析给出了轻骨料钢管混凝土短柱火灾后轴压承载力的计算公式。结果表明,以轻质材料———陶粒为骨料制成的钢管混凝土短柱受火后仍然具有较高的承载力和良好的延性,而当钢管表面响应温度超过500℃时,试件的轴压承载力出现了明显的下降。所给出的轻骨料钢管混凝土柱火灾后的轴压承载力计算公式可作为该类构件火灾后修复计算的参考依据。 相似文献
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184.
Sari Kontunen-Soppela Juha Parviainen Mikael Brosché Ramesh C. Thakur Jaakko Kangasjärvi David F. Karnosky 《Environmental pollution (Barking, Essex : 1987)》2010,158(4):959-968
Gene expression responses of paper birch (Betula papyrifera) leaves to elevated concentrations of CO2 and O3 were studied with microarray analyses from three time points during the summer of 2004 at Aspen FACE. Microarray data were analyzed with clustering techniques, self-organizing maps, K-means clustering and Sammon's mappings, to detect similar gene expression patterns within sampling times and treatments. Most of the alterations in gene expression were caused by O3, alone or in combination with CO2. O3 induced defensive reactions to oxidative stress and earlier leaf senescence, seen as decreased expression of photosynthesis- and carbon fixation-related genes, and increased expression of senescence-associated genes. The effects of elevated CO2 reflected surplus of carbon that was directed to synthesis of secondary compounds. The combined CO2 + O3 treatment resulted in differential gene expression than with individual gas treatments or in changes similar to O3 treatment, indicating that CO2 cannot totally alleviate the harmful effects of O3. 相似文献
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186.
Moens LN Smolders R van der Ven K van Remortel P Del-Favero J De Coen WM 《Chemosphere》2007,67(11):2293-2304
Effluents are a main source of direct and continuous input of pollutants to the aquatic environment, and can cause ecotoxicological effects at different levels of biological organization. Since gene expression responses represent the primary interaction site between environmental contaminants and biota, they provide essential clues to understand how chemical exposure can affect organismal health. The aim of the present study was to investigate the applicability of a microarray approach for unraveling modes of action of whole effluent toxicity and impact assessment. A chronic toxicity test with common carp (Cyprinus carpio) was conducted where fish were exposed to a control and 100% effluent for 21 days under flow-through conditions. Microarray analysis revealed that effluent treatment mainly affected molecular pathways associated with the energy balance of the fish, including changes in carbohydrate and lipid metabolism, as well as digestive enzyme activity. These gene expression responses were in clear agreement with, and provided additional mechanistic information on various cellular and higher level effects observed for the same effluent. Our results demonstrate the benefit of toxicogenomic tools in a “systems toxicology” approach, involving the integration of adverse effects of chemicals and stressors across multiple levels of biological complexity. 相似文献
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188.
构建了含早期、晚期、极晚期启动子、全期表达HBsAg 基因的重组杆状病毒vTnNPVP35HBsOCC+ .结果HBsAg 表达时间由感染后12 h 提前到4 h,而其表达量,特别是病毒感染早期的表达水平亦显著提高,其中24 h 和96 h 的表达量分别比对照提高180 % 和60 % . 转录起始位点分析结果显示,该重组毒株可在病毒感染的早、晚期形成多个转录本 相似文献
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190.
为揭示SigL及其增强子结合蛋白(EBPs)在苏云金芽胞杆菌(Bt)中的调控功能,在全基因组测序的基础上,采用生物信息学方法,对YBT-1520菌株的SigL及其EBPs的结构和功能进行深入分析.结果表明,YBT-1520基因组中存在1个SigL和6个EBPs,而且EBPs在结构上具有丰富的多样性,包含了EBPs的所有可能的结构域组织类型.SigL所调控的基因涉及11个假定的COG代谢途径,其中包括能量代谢、氨基酸代谢、翻译与细胞周期等.根据EBPs在基因组的位置推测,YBT-1520的EBPs参与γ-氨基丁酸代谢途径、精氨酸代谢途径、支链脂肪酸降解途径、多糖分解代谢等代谢途径的调控.本研究将为揭示Bt杀虫晶体蛋白大量表达的调控机制提供新的思路. 相似文献