猪粪堆肥发酵菌种的分离筛选及特性研究

从多种样品材料中分离筛选出8株能以猪粪为基质生长的菌种,对其中4株菌(8B、8C、8E、2B)进行了初步分类鉴定,并对它们发酵猪粪培养基的特性作了研究.结果表明,这4株菌均属于芽孢杆菌属,其中8C、2B菌种生长最好,8B次之,而8E菌种则不适合作为发酵菌种;对培养基采取完全灭菌对菌种生长繁殖是有利的,间歇灭菌和不灭菌效果相对较差,甚至会对菌种产生不利影响;pH值的变化在一定程度上反映出微生物生长繁殖能力的强弱;较低的pH值不利于微生物菌种生长.     

不同墩高下连续刚构桥抗震性能分析

以某大跨连续高墩刚构桥为依托,采用Midas/civil软件建立仿真模型,通过时程分析方法研究双肢薄壁墩参数在地震响应下对高墩连续刚构桥抗震性能的影响.以桥墩高度为主要参数,以模型阵型频率、桥墩墩顶、墩底位移及内力变化三个方面对其抗震性能进行评价.研究结果表明,随着其中一个桥墩高度的增加,桥梁频率不断减小,矮墩墩底及矮...     

Leaching of styrene and other aromatic compounds in drinking water from PS bottles

Bottled water may not be safer, or healthier, than tap water. The present studies have proved that styrene and some other aromatic compounds leach continuously from polystyrene （PS） bottles used locally for packaging. Water sapmles in contact with PS were extracted by a preconcentration technique called as ＂purge and trap＂ and analysed by gas chromatograph-mass spectrometer （GC/MS）. Eleven aromatic compounds were identified in these studies. Maximum concentration of styrene in PS bottles was 29.5 μg/L. Apart from styrene, ethyl benzene, toluene and benzene were also quantified but their concentrations were much less than WHO guide line values. All other compounds were in traces. Quality of plastic and storage time were the major factor in leaching of styrene. Concentration of styrene was increased to 69.53 μg/L after one-year storage. In Styrofoam and PS cups studies, hot water was found to be contaminated with styrene and other aromatic compounds. It was observed that temperature played a major role in the leaching of styrene monomer from Styrofoam cups. Paper cups were found to be safe for hot drinks.     

Non-UV based germicidal activity of metal-doped TiO2 coating on solid surfaces

A stain-based screening method was developed to screen different catalyst coatings for their germicidal activity. A Baclight dead/live bacteria viability kit （invitrogen, molecular probes） was used for staining the cell. The screening was carried out following a standard procedure. This included loading cell suspension to solid surface and maintaining contact for 30 min, then staining with a mixture containing dyes. The stained cells were observed using an epifluorescent microscope and photographed with a CCD camera under UV. Metal-doped TiO2 coatings on AI plates were prepared and tested for non-UV germicidal activity without using UV. It was tested using model microorganisms such as Bakers Yeast （Saccharomyces cerevisiae）, Bacillus subtilis, Pseudomonas putida, and Escherichia coli. On the basis of the germicidal activity of catalyst and the degree of damage caused to the cells, the stained cells may appear green （viable）, green with red or yellow nuclei and yellow （compromised） or red （nonviable）. According to their stained color, cells were counted to calculate the percentage of dead, live, and compromised cells. Compromised cells are cells that grow very slowly after reculturing indicating a degree of reversible cell damage. Screening the germicidal activity using this staining method is accurate and efficient, and requires less time than the culture-based method. A modification to the procedure for measuring germicidal activity of rough surfaces or fibrous coatings was developed. Both TiO2 and metal-doped TiO2 （Ag, Pt, Au, Cu） possess non-UV based germicidal activity. The germicidal activity of TiO2 was found to be related with its wetting property and can be improved by UV irradiation before testing. It is not greatly affected by contact time, indicating a fast acting germicidal activity.     

降水量时间序列变化的小波特征

利用小波变换对降水量时间序列的多时间尺度变化及突变特征进行了探讨。小波变换不仅能将降水量时间序列的频率特征在时间域上展现出来，清晰地给出各种时间尺度的强弱和分布情况以及早涝变化趋势和突变点，而且还能分析出其主要周期。以新安江流域黄山地区主汛期(5—7月)和年降水量为例，计算表明，其年际及年代际时间尺度在时域中分布不均匀，具有明显的局部化特征；同时分析出主汛期降水具有8年、19年左右的周期，年降水存在6年、19年左右的周期；研究还表明，主汛期降水与年降水的时间尺度变化比较接近。     

Optimal Reorganization of NASA Earth Science Data for Enhanced Accessibility and Usability for the Hydrology Community

A long‐standing “Digital Divide” in data representation exists between the preferred way of data access by the hydrology community and the common way of data archival by earth science data centers. Typically, in hydrology, earth surface features are expressed as discrete spatial objects (e.g., watersheds), and time‐varying data are contained in associated time series. Data in earth science archives, although stored as discrete values (of satellite swath pixels or geographical grids), represent continuous spatial fields, one file per time step. This Divide has been an obstacle, specifically, between the Consortium of Universities for the Advancement of Hydrologic Science, Inc. and NASA earth science data systems. In essence, the way data are archived is conceptually orthogonal to the desired method of access. Our recent work has shown an optimal method of bridging the Divide, by enabling operational access to long‐time series (e.g., 36 years of hourly data) of selected NASA datasets. These time series, which we have termed “data rods,” are pre‐generated or generated on‐the‐fly. This optimal solution was arrived at after extensive investigations of various approaches, including one based on “data curtains.” The on‐the‐fly generation of data rods uses “data cubes,” NASA Giovanni, and parallel processing. The optimal reorganization of NASA earth science data has significantly enhanced the access to and use of the data for the hydrology user community.     

Recent Changes in Stream Flashiness and Flooding,and Effects of Flood Management in North Carolina and Virginia

The southeastern United States has undergone anthropogenic changes in landscape structure, with the potential to increase (e.g., urbanization) and decrease (e.g., reservoir construction) stream flashiness and flooding. Assessment of the outcome of such change can provide insight into the efficacy of current strategies and policies to manage water resources. We (1) examined trends in precipitation, floods, and stream flashiness and (2) assessed the relative influence of land cover and flow‐regulating features (e.g., best management practices and artificial water bodies) on stream flashiness from 1991 to 2013. We found mean annual precipitation decreased, which coincided with decreasing trends in floods. In contrast, stream flashiness, overall, showed an increasing trend during the period of study. However, upon closer examination, 20 watersheds showed stable stream flashiness, whereas 5 increased and 6 decreased in flashiness. Urban watersheds were among those that increased or decreased in flashiness. Watersheds that increased in stream flashiness gained more urban cover, lost more forested cover and had fewer best management practices installed than urban watersheds that decreased in stream flashiness. We found best management practices are more effective than artificial water bodies in regulating flashy floods. Flashiness index is a valuable and straightforward metric to characterize changes in streamflow and help to assess the efficacy of management interventions.     

Robustness of Meteorological Droughts in Dynamically Downscaled Climate Simulations

We examine the robustness of a suite of regional climate models (RCMs) in simulating meteorological droughts and associated metrics in present‐day climate (1971‐2003) over the conterminous United States (U.S.). The RCMs that are part of North American Regional Climate Change Assessment Program (NARCCAP) simulations are compared with multiple observations over the climatologically homogeneous regions of the U.S. The seasonal precipitation, climatology, drought attributes, and trends have been assessed. The reanalysis‐based multi‐model median RCM reasonably simulates observed statistical attributes of drought and the regional detail due to topographic forcing. However, models fail to simulate significant drying trend over the Southwest and West. Further, reanalysis‐based NARCCAP runs underestimate the observed drought frequency overall, with the exception of the Southwest; whereas they underestimate persistence in the drought‐affected areas over the Southwest and West‐North Central regions. However, global climate model‐driven NARCCAP ensembles tend to overestimate regional drought frequencies. Models exhibit considerable uncertainties while reproducing meteorological drought statistics, as evidenced by a general lack of agreement in the Hurst exponent, which in turn controls drought persistence. Water resources managers need to be aware of the limitations of current climate models, while regional climate modelers may want to fine‐tune their parameters to address impact‐relevant metrics.     

Highly efficient conversion of sugarcane bagasse pretreated with liquid hot water into ethanol at high solid loading

Liquid hot water (LHW), an environmental-friendly physico-chemical treatment, was applied to pretreat the sugarcane bagasse (SCB). Tween80, a non-ionic surfactant, was used to enhance the enzymatic hydrolysis of the pretreated SCB. It found that 0.125 mL Tween80 /g dry matter could make the maximum increase (33.2%) of the glycan conversion of the LHW-pretreated SCB. A self-designed laboratory facility with a plate-and-frame impeller was applied to conduct batch hydrolysis, fed-batch hydrolysis, and the process of high-temperature (50°C) fed-batch hydrolysis following low-temperature (30°C) simultaneous saccharification and fermentation (SSF) which was adopted to overcome the incompatible optimum temperature of saccharification and fermentation in the SSF process. After hydrolyzing LHW-pretreated SCB for 120 h with commercial cellulase, the total sugar concentration and glycan conversion obtained from fed-batch hydrolysis were 91.6 g/L and 68.3%, respectively, which were 9.7 g/L and 7.3% higher than those obtained from batch hydrolysis. With Saccharomyces cerevisiae Y2034 fermenting under the non-sterile condition, the ethanol production and theoretical yield obtained from the process of SSF after fed-batch hydrolysis were 55.4 g/L and 88.3% for 72h, respectively, which were 15.5 g/L and 24.7% higher than those from separate fed-batch hydrolysis and fermentation. The result of this work was superior to the reported results obtained from the LHW-pretreated SCB.