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801.
Summary. Under field conditions significantly more black
chafer, Holotrichia loochooana loochooana (Sawada)
(Coleoptera: Scarabaeidae) males landed on black and gray
sources than white ones containing 10 mg of female
pheromone, anthranilic acid. When a broader spectrum of
colours was tested the frequency of male landing was intensively
proportional to as the lightness of the colour of the
lures. These findings demonstrated that mate location by
H. l. loochooana males is dependent on
both olfactory and visual stimuli of the source. In contrast, female aggregation was not
affected by colour. 相似文献
802.
Dr Haruo Takabayashi Soryu Kuwabara Toshihiko Ukita Kazumi Ikawa Kaoru Yamafuji Tatsuhiro Igaras 《黑龙江环境通报》1995,15(1):74-77
Several attempts have been made to detect and retrieve fetal nucleated cells including nucleated erythrocytes (NRBCs), leukocytes, and trophoblasts in maternal blood. We have recently developed a new method for non-invasive fetal DNA diagnosis from maternal blood. Peripheral blood granulocytes including NRBCs were isolated by a discontinuous density gradient method using Percoll (Pharmasia). NRBCs were found and retrieved at a single cell level using a micromanipulator under a microscope. To determine whether the origin of the NRBCs was maternal or fetal, the NRBCs were analysed by polymerase chain reaction (PCR) amplification to determine the presence of a Y-chromosome-specific repeat sequence in mothers carrying male fetuses. We were successful in predicting fetal sex accurately in 10 out of 11 samples taken from maternal blood. This new technique opens up fetal DNA diagnosis from maternal blood during the first trimester of pregnancy to the whole population because there is no risk to the fetus or the mother. 相似文献
803.
洞庭平原褐家鼠种群动态和繁殖特性 总被引:8,自引:1,他引:7
褐家鼠是洞庭平原稻作区的主要害鼠之一,在房舍区数量动态复杂,季节消长基本上属春冬双峰型,在农田区数量季节消长为夏秋双峰型。褐家鼠总雌性比为52.0%,全年繁殖.3~10月为繁殖盛期,盛期平均怀孕率为34.91±8.69%,平均胎仔数为8.2±l.0,4、7、9月出现怀孕高峰,11月至翌年2月为繁殖低潮期。褐家鼠在洞庭平原70年代数量较低,80年代初暴发成灾,80年代后期数量有逐步下降趋势。针对种群特征,提出了控制褐家鼠种群数量的对策。 相似文献
804.
805.
806.
通过正交实验,研究了超声破碎法和低温高压破碎法提取一氧化氮还原酶(nitric oxide reductase,nor)的活性,分析了超声强度和次数,破碎压力和次数,裂解液的添加量等因素对于胞内可溶性蛋白和核酸(DNA)的释放与提取nor活性的影响.根据活性污泥中酶的催化特性以及其他实验条件,完善了nor催化活性的测定方法.结果表明超声法提取nor的参数应设定为,超声次数为100次,超声强度为500W,裂解液的添加量为0.1mL.低温高压破碎法提取nor的参数应设定为破碎次数为4次,破碎压力为50MPa,裂解液的添加量为0.1mL.低温高压破碎法下的胞内可溶性蛋白和DNA的释放量与nor的最大催化活性要高于超声破碎法.此外,在nor催化活性的测定方法中,nor活性测定终点时间应为15min. 相似文献
807.
Yuan Li Mark A. Taggart Craig McKenzie Zulin Zhang Yonglong Lu Sabolc Pap Stuart W. Gibb 《环境科学学报(英文版)》2021,33(2):18-27
This work describes the development, optimisation and validation of an analytical method for the rapid determination of 17 priority pharmaceutical compounds and endocrine disrupting chemicals (EDCs). Rather than studying compounds from the same therapeutic class, the analyses aimed to determine target compounds with the highest risk potential (with particular regard to Scotland), providing a tool for further monitoring in different water matrices. Prioritisation was based on a systematic environmental risk assessment approach, using consumption data; wastewater treatment removal efficiency; environmental occurrence; toxicological effects; and pre-existing regulatory indicators. This process highlighted 17 compounds across various therapeutic classes, which were then quantified, at environmentally relevant concentrations, by a single analytical methodology. Analytical determination was achieved using a single-step solid phase extraction (SPE) procedure followed by high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). The fully optimised method performed well for the majority of target compounds, with recoveries >71% for 15 of 17 analytes. The limits of quantification for most target analytes (14 of 17) ranged from 0.07 ng/L to 1.88 ng/L in river waters. The utility of this method was then demonstrated using real water samples associated with a rural hospital/setting. Eight compounds were targeted and detected, with the highest levels found for the analgesic, paracetamol (at up to 105,910 ng/L in the hospital discharge). This method offers a robust tool to monitor high priority pharmaceutical and EDC levels in various aqueous sample matrices. 相似文献
808.
A gas‐liquid‐chromatographic procedure for the quantitative determination of authentic carazolol and carazolol in tablets (Conducton(R)?) has been described. Carazolol in tablets was extracted with absolute alcohol before injecting onto the gas Chromatograph. The concentration range adopted varied between 0.2 mg to 1.0 mg/ml of carazolol in alcohol solution. The results obtained were 97.6%±3.2 and 98.4%±2.6 for the stated and added amounts respectively. 相似文献
809.
气相色谱法测定沼气中的甲烷 总被引:1,自引:0,他引:1
用气相色谱法对沼气成份中的甲烷进行测试。色谱柱为Porapak Q填充柱,载气为氩气,最佳工作条件为:柱温40℃,检测器温度180℃,进样器温度100℃,桥电流50 mA,载气流速20.3 mL/min,并利用外标法建立工作曲线。结果表明:所选色谱条件能够快速准确的对沼气进行测试,分离效果好,有较高的重复性和准确度,适合大量样品的测定。 相似文献
810.
鱼类性别分化是在性别决定机制的控制下,鱼类未分化的性腺向卵巢或精巢发育,并出现第2性征的过程,受自身内分泌系统的精确调控。环境内分泌干扰物能通过干扰内源激素的合成、释放、转运、代谢、结合和作用等过程影响鱼类的内分泌机能,从而对鱼类的性别分化产生影响。介绍了鱼类性别分化的模式和过程以及内分泌系统对鱼类性别分化的调控作用,综述了环境内分泌干扰物对鱼类性别比例、配子发生、性腺发育和第2性征的影响,从性类固醇激素的合成以及性别分化相关基因的表达两个方面探讨了环境内分泌干扰物影响鱼类性别分化的作用机制,并展望了该领域未来的研究方向。 相似文献