Detection of fetal cells in maternal blood

We report the detection of fetal cells in the maternal circulation by enzymatic amplification of a single copy gene sequence that was fetal-specific. Fetal HLA-A2-positive cells were sorted from maternal HLA-A2-negative cells by flow cytometry and confirmed by demonstration of a fetal-specific HLA-DR4 sequence. However, this sequence could not be detected in unenriched maternal DNA prepared at 28 and 32 weeks' gestation. The sensitivity of detection was 1 HLA-DR4-positive cell in 10⁵ HLA-DR4-negative cells. We conclude that prenatal diagnosis of paternally inherited autosomal-dominant genetic defects may be possible by selective gene amplification of maternal peripheral blood. However, preliminary enrichment for fetal cells may be necessary.     

Polymerase chain reaction analysis of the cystic fibrosis ΔF508 mutation in human blastomeres following oocyte injection of a single sperm from a carrier

The efficiency of the polymerase chain reaction (PCR) in detecting the cystic fibrosis (CF) ΔF508 mutation (which is the most common mutation of CF) was assessed in single human blastomeres. Twenty-one human immature oocytes (germinal-vesicle-stage oocytes) that had been donated for research were matured in vitro and a single spermatozoon from a carrier of the CF ΔF508 mutation was injected into the ooplasm. Fourteen embryos were obtained after intracytoplasmic sperm injection (ICSI). PCR analysis was carried out on 70 single blastomeres isolated from these 14 embryos. The results showed that the efficiency of DNA amplification by PCR in single nucleate blastomeres was 94 per cent (59/63). There were no false-positive results since none of the blank samples or the blastomeres without a nucleus showed an amplified signal. We found that nine embryos were homozygous for the unaffected genotype and that four embryos were heterozygous since they contained both the unaffected and the ΔF508 genotype. In a four-cell embryo, we observed the homozygous unaffected genotype in one blastomere and a heterozygous ΔF508/unaffected genotype in the other three blastomeres.     

"威望"号轮油污事件对航运业的警示

通过对“威望”轮油污事件的分析，提出当务之急是从有关薄弱环节着手，强化安全责任链意识和机制，创建船舶安全信息保障系统，常抓不懈，跳出事故周期律等的相应对策，以遏止目前海上频发的油轮事故。     

绍兴市城市工业生态规划转型的研究

环境与经济协调发展是当代各国面临的重大问题和挑战,生态工业是协调这一问题和挑战的重要战略,是可持续发展的基础之一.根据绍兴市以印染工业为主导、多种行业共同发展的产业结构,结合循环经济的理论和工业生态规划的原理和技术方法,来探讨绍兴市城市工业生态规划的转型模式,提出绍兴市工业结构调整的对策、生态工业网络体系的技术设计的策略以及生态工业园的产品链设计方案.绍兴市工业生态规划的转型是实现城市生态工业系统的有效可行的途径,是生态工业的集中体现.     

资源安全及其基本属性与研究框架

国内外对资源安全问题的关注由来已久,但系统研究此问题还是近年的事情。在此,对资源安全问题的由来、基本属性、研究框架等进行系统论述。认为资源安全是一个国家或地区可以持续、稳定、及时、足量和经济地获取所需自然资源的状态或能力;资源安全具有数量、质量、结构、均衡和价格等方面的含义;并对资源安全按过程、主体和类别等进行了分类;提出应树立开放、动态、持续和系统的资源安全观;资源安全研究应主要包括资源安全概念与属性、资源安全测度指标体系与预警系统、国家资源安全态势、国家资源安全战略等内容。     

开发有机食品促进我省农业可持续发展

该文通过分析我省发展有机食品的有利及不利因素，提出保证我省有机食品健康发展的对策。     

基于(火用)分析法的矿区工业生态链物质能量流动研究

从工业生态学角度研究矿区工业生态系统的工业代谢和工业生态链,建立矿区物质循环与能量流动(火用)分析和(火用)效率模型.根据生态效率的理念,矿区发展需要五项关键支撑技术,为合理选择煤炭的产业链延伸途径打下理论基础,促进矿区可持续发展.     

南海生态工业园区的生态规划

南海生态工业园区的生态规划 ,以循环经济理论和工业生态学原理为指导 ,通过园区的景观生态规划、工业生态系统规划和生态管理规划等措施 ,达到构建可持续性工业园区、发展生态工业的目的     

有机食品的发展现状及趋势探讨

介绍了有机食品的概念,分析了国际有机食品运动的发展方向、推动因素和市场潜力,预测今后几年内全球有机产品市场的年平均发展速率为15％－20％。同时,还分析了我国发展有机食品的国际环境、农业基础和发展模式,认为发展有机农业和有机食品是跨越绿色贸易壁垒的良策,国内的消费市场潜力和空间巨大,预测到2010年,我国有机食品占国内食品市场的比例可望达到1.0%-1.5%,占全球有机食品市场的份额则可望达到3.0%。