基于人工蜂群算法的边坡最危险滑动面搜索

将用于连续数值优化问题的人工蜂群算法引入边坡稳定分析临界滑动面搜索领域.该方法模拟了蜂群的群体协作采蜜过程,具有自适应收敛的特点,克服了传统方法容易陷入局部最优的缺点,是一种全局优化算法.为进一步改善其在复杂边坡搜索中的效果,将Hooke-Jeeves模式搜索操作引入人工蜂群算法,提出一种用于边坡临界滑动面搜索的模式搜...     

The Structure of Narrow-Skulled Vole (Microtus gregalis Pall.) Colonies in Western Siberia

The structure of Microtus gregalis colonies was studied in different parts of the species range in Western Siberia, both in the south (Omsk oblast) and in the north (Yamalo-Nenets Autonomous Area), in 1999 and 2000. The colonies studied in the steppe zone of Omsk oblast, southern tundras of the Yamal Peninsula, and mountain tundras on the northern slopes of the Polar Urals prove to have some common structural features. In Omsk oblast, seasonal variation in the area of colonies and the number of entrances to the burrows was revealed, although the general structure of colonies (the pattern of tunnels and location of the nest and storage chambers) was largely the same in spring and autumn.     

Isolation,identification, Pb(Ⅱ) biosorption isotherms and kinetics of a lead adsorbing Penicillium sp. MRF-1 from South Korean mine soil

A heavy metal contaminated soil sample collected from a mine in Chonnam Province of South Korea was found to be a source of heavy metal adsorbing biosorbents. Chemical analyses showed high contents of lead (Pb) at 357 mg/kg and cyanide (CN) at 14.6 mg/kg in the soil. The experimental results showed that Penicillium sp. MRF-1 was the best lead resistant fungus among the four individual metal tolerant fungal species isolated from the soil. Molecular characterization of Penicillium sp. MRF-1 was determined using ITS regions sequences. E ects of pH, temperature and contact time on adsorption of Pb(II) by Penicillium sp. MRF-1 were studied. Favorable conditions for maximum biosportion were found at pH 4 with 3 hr contact time. Biosorption of Pb(II) gradually increased with increasing temperature. E cient performance of the biosorbent was described using Langmuir and Freundlich isotherms. Adsorption kinetics was studied using pseudo first-order and pseudo second-order models. Biosorbent Penicillium sp. MRF-1 showed the maximum desorption in alkali conditions. Consistent adsorption/desorption potential of the biosorbent in repetitive cycles validated the e cacy of it in large scale. SEM studies given notes on surface modification of fungal biomass under metal stress and FT-IR results showed the presence of amino groups in the surface structure of the biosorbent. In conclusion, the new biosorbent Penicillium sp. MRF-1 may potentially be used as an inexpensive, easily cultivatable material for the removal of lead from aqueous solution.     

白腐真菌细胞色素P450的诱导及检测方法研究

以CO结合差光谱为基本检测法,研究了白腐真菌黄孢原毛平革菌中细胞色素P450的诱导和适宜的分离检测方法.结果表明,正己烷对该真菌P450有显著的诱导作用,P450诱导量受正己烷浓度和诱导时间的影响,每小时投加正己烷2μL/mL、诱导6 h可使该真菌微粒体P450比浓度高达140~160 pmol/mg.在此基础上,本研究优化了分离P450时破碎细胞的方法以及光谱法检测P450的条件.破碎细胞时,采用高速分散结合玻璃研磨,比采用玻璃研磨、超声破碎和珠磨等方法分离的微粒体P450含量高1~5倍,是更为适宜的破碎方法.检测CO结合差光谱时,通气和还原条件对P450检测值有显著影响,较适宜的条件为:样品池和对照池分别通入等量的CO和N2,通气流量为3 mL/min(300μL样本),通气时间40 s;通气后投加还原剂低亚硫酸钠,投加浓度为0.4 mol/L.     

开放体系下产酸白腐菌连续处理造纸黑液研究

研究了产酸白腐菌CoriolusSp.S1在开放环境下连续曝气处理造纸黑液。在连续50d处理过程中,产酸白腐菌可以稳定的连续处理黑液,与进水相比,出水中COD降幅最高达到53.73%,脱色率平均可以达到73.74%,木质素去除率达到45.66%,为白腐菌应用于造纸黑液的治理提供了一定的基础。     

Desulfurization of dibenzothiophene（DBT） by a novel strain Lysinibacillus sphaericus DMT-7 isolated from diesel contaminated soil

A new bacterial strain DMT-7 capable of selectively desulfurizing dibenzothiophene (DBT) was isolated from diesel contaminated soil. The DMT-7 was characterized and identified as Lysinibacillus sphaericus DMT-7 (NCBI GenBank Accession No. GQ496620) using 16S rDNA gene sequence analysis. The desulfurized product of DBT, 2-hydroxybiphenyl (2HBP), was identified and confirmed by high performance liquid chromatography analysis and gas chromatography-mass spectroscopy analysis respectively. The desulfurization kinetics revealed that DMT-7 started desulfurization of DBT into 2HBP after the lag phase of 24 hr, exponentially increasing the accumulation of 2HBP up to 15 days leading to approximately 60% desulfurization of the DBT. However, further growth resulted into DBT degradation. The induced culture of DMT-7 showed shorter lag phase of 6 hr and early onset of stationary phase within 10 days for desulfurization as compared to that of non-induced culture clearly indicating the inducibility of the desulfurization pathway of DMT-7. In addition, Lysinibacillus sphaericus DMT-7 also possess the ability to utilize broad range of substrates as sole source of sulfur such as benzothiophene, 3,4-benzo DBT, 4,6-dimethyl DBT, and 4,6-dibutyl DBT. Therefore, Lysinibacillus sphaericus DMT-7 could serve as model system for efficient biodesulfurization of diesel and petrol.     

霉菌吸附水体中Cr(Ⅵ)Cd(Ⅱ)离子研究

采用从活性污泥中筛选的ZYL霉菌,进行吸附水体中Cr(Ⅵ)、Cd(Ⅱ)离子研究。研究结果表明:在Cr(Ⅵ)、Cd(Ⅱ)浓度分别为300mg/L时,菌种生长良好。吸附水体中[Cr(Ⅵ)、Cd(Ⅱ)]的最佳条件是pH=5.0,时间1h,温度为10℃。吸附规律符合Langm uir等温吸附模型,由回归方程得到Cr(Ⅵ)的表观最大吸附量为14m g/g;Cd(Ⅱ)的表观最大吸附量为52m g/g,说明该霉菌可以很好的去除低温水体(地下水)中Cr(Ⅵ)、Cd(Ⅱ)离子。     

重金属对禾谷镰刀菌的生长及毒素合成的影响

以禾谷镰刀菌为研究对象,研究了常见重金属(Cu、Pb、Zn、Cd)对禾谷镰刀菌菌株生长及其毒素合成的影响。研究结果表明,重金属对菌株的生长和其产毒能力均产生影响。Cu~(2+)和Cd~(2+)对菌株生长影响较大,随着浓度的增加对生长的抑制作用增强,当离子浓度分别为20 mg·L~(-1)和40 mg·L~(-1)时,能够完全抑制菌株生长。Zn~(2+)在0~160 mg·L~(-1)浓度范围内促进菌株生长,在10 mg·L~(-1)浓度下促进毒素合成。Pb~(2+)在察氏培养基中对菌株生长的影响没有明显规律可循,但是,随着Pb~(2+)浓度增加,抑制毒素合成作用增强。     

基于蚁群神经网络的煤矿瓦斯预测模型

为了提高煤矿瓦斯涌出量的预测精度和预测速度,用蚁群算法和神经网络相结合的方法进行预测模型设计。选择瓦斯涌出的重要影响因素,建立其神经网络的预测模型。以网络的均方误差为目标函数,通过蚁群算法的迭代运算,实现BP网络的权值优化,并用优化好的BP网络进行瓦斯涌出预测。仿真结果表明,该方法具有较高的拟合预测精度。     

基于蚁群算法的石化项目应急路径规划研究

蚁群算法是一种智能仿生优化算法,实际运用仿真模拟时具有良好的寻优性和高效性。将蚁群算法与石化项目的路径规划结合,借鉴蚁群算法解决旅行商问题TSP的思路并将之运用于应急路径规划,利用MATLAB仿真软件,以某石化项目的应急路径规划为实例,较好地实现了有关应急路径模拟图,可为有关石化项目的安全规划提供借鉴和参考。