根际微生物耦合降解系统的构建及其对蒽污染土壤的修复

摘要将增强型绿色荧光蛋白(EGFP)标记的黄麻土生根际优势菌Tu-1B分别与葸高效降解菌An-2和生物表面活性剂产生菌P7-50进行原生质体电融合，得到两株具有荧光标记的融合子Tu-An和Tu-P．将二融合子接种于植物根际土壤，构建根际微生物耦合降解系统，：在40d时该系统的最大降解率为96％．对根际土壤中的Tu-An融合子进行了检测，结果表明融合子在根际能稳定旺盛生长．图3表4参18     

原生质体融合构建高产碱性蛋白酶工程菌

用原生质体融合的方法，将碱性蛋白酶生产菌2709与含有碱性蛋白酶基因克隆载体pDW2的工程菌枯草杆菌BD105进行细胞融合，得到1株高产碱性蛋白酶的工程菌A16，菌落的原位杂交表明，该菌株携有双亲的遗传物质，A16的表型与生长特征与2709相似，但同样条件下发酵酶活比2709高50%-100%，摇瓶发酵的产酶水平最高可达30000u/mL,A16发酵所产酶的最适pH与耐温性及发酵条件等特征均与2709相同。适用于工业生产。     

Feasible conversion of solid waste bauxite tailings into highly crystalline 4A zeolite with valuable application

Bauxite tailings are a major type of solid wastes generated in the flotation process. The waste by-products caused significant environmental impact. To lessen this hazardous effect from poisonous mine tailings, a feasible and cost-effective solution was conceived and implemented. Our approach focused on reutilization of the bauxite tailings by converting it to 4A zeolite for reuse in diverse applications. Three steps were involved in the bauxite conversion: wet-chemistry, alkali fusion, and crystallization to remove impurities and to prepare porous 4A zeolite. It was found that the cubic 4A zeolite was single phase, in high purity, with high crystallinity and well-defined structure. Importantly, the 4A zeolite displayed maximum calcium ion exchange capacity averaged at 296 mg CaCO₃/g, comparable to commercially-available zeolite (310 mg CaCO₃/g) exchange capacity. Base on the optimal synthesis condition, the reaction yield of zeolite 4A from bauxite tailings achieved to about 38.43%, hence, this study will provide a new paradigm for remediation of bauxite tailings, further mitigating the environmental and health care concerns, particularly in the mainland of PR China.     

运营期地铁隧道结构安全信息融合监控体系初探

以隧道结构系统特性研究为基础,以从单一正向思维模式向逆向思维、系统思维、反馈思维等全方位思维模式的转变为核心,引入并讨论了基于信息融合、分析融合、诊断融合和评价融合的体系结构,探索性地研究了运营期地铁隧道结构安全信息融合监控理论和方法体系。     

基于图像感兴趣区域融合特征的PM2.5浓度预测方法

通过图像预测PM_2.5浓度的准确性,在很大程度上取决于模型所选用的特征参数。为丰富特征参数的表达,设计了一种基于图像传统特征与深度特征充分融合的PM_2.5浓度预测方法。首先,根据不同PM_2.5浓度下的成像差异,选定图像感兴趣区域,解决图像尺寸过大导致的模型运算效率较低问题。然后,针对所选取的局部图像,利用传统图像处理方法手动设计并提取图像浅表视觉特征,同时利用卷积神经网络自动提取图像深层语义特征。最后,将两种特征融合,交由卷积神经网络的全连接层实现对PM_2.5浓度的回归预测。预测误差比对结果显示,相比使用单种特征,使用融合特征能够有效提高模型的预测性能。     

基于Agent的长输管道应急指挥决策系统设计

为帮助决策机构快速高效地制定长输管道事故应急救援方案和行动目标,运用Agent (真体)对长输管道应急指挥决策系统进行设计.通过划分应急指挥系统层次,分析信息融合Agent、形势评估Agent、资源规划Agent这3个关键模块,建立基于Agent的应急指挥过程模型.结合应急指挥机构和应急指挥决策系统,提出应急指挥系统...     

一种基于游弋图像的水域监视鸟瞰图成像系统

针对各类水域污染日趋严重而传统水质监测方式离散、范围受限等不足,构建了基于游弋图像的水域鸟瞰图成像系统,实现大范围实时、连续监控。使用偏振设备消除水面反光,得到清晰的水面图像。设计了摄像机内外参标定的方法,在线获得状态参数;提出了自适应空洞填补的策略,消除了由透视图映射至鸟瞰图时产生的图像空洞;结合GPS信息和图像特征配准技术,获取了由船载摄像机视频序列拼合成的鸟瞰全景图像。该方法具有分辨率高、实时性强、监测范围广和使用成本低等优势。     

汽车驾驶员驾驶疲劳监测技术研究进展

当今驾驶疲劳已成为引发交通事故的主要原因，但由于其形成原因复杂，监测技术和手段都还不成熟。驾驶疲劳客观监测技术是目前研究的热点，主要包括对驾驶员脑电图、心电图、眼睑眨动、头部运动轨迹、方向盘的运动、车辆行驶方向等的监测技术。由于上述监测技术都存在一定的局限性，因此，笔者认为，今后的研究应该从以下4个方面着手进行：①研制非接触性高灵敏度传感器；②寻求新的监测指标和方法；③将多传感器信息融合技术应用于监测技术中；④研制高性价比的监测装置。     

垃圾焚烧炉燃烧过程的优化控制策略

焚烧炉控制旨在使城市垃圾经处理后达到无害化、减量化和资源化的目标,既有利于环境保护,也有良好的社会经济效益。针对垃圾焚烧过程中由于燃烧对象复杂性导致的控制难题,提出了一种对垃圾焚烧系统实施优化控制的仿人智能控制策略。研究出稳定燃烧评判指标、控制模型、工程控制算法,并进行仿真试验。系统仿真试验结果表明:仿人智能控制策略鲁棒性强、控制品种好,是一种有效的控制策略。     

Protoplast fusion between Saccharomyces cerevisiae and Rhodobacter sphaeroides for the treatment of soybean wastewater

The kinetic parameters and the purification rates of the hybrid cell Foaz in soybean processing wastewater (SPW) were measured through a shaking reaction and in two automatic regulated control stable fermentation systems (ARCSFS). The maximum specific growth rate of Foaz was 0.576 h^‐1, higher than that of one of its parental strains Rhodobacter sphaeroides P9479 and lower than that of another parental strain Saccharomyces cerevisiae Y9407. The BOD₅ removal rate of Foaz in the No. 1 system was 61.3%, higher than those of both its parental strains when the influent BOD₅ concentration was 4600 mg/L. The results of this study suggest that the hybrid Foaz has a better capacity of the degradation of organic pollutants in SPW than its parental strains and it may be applicable to the treatment of high concentration organic wastewater.