一种工程菌的高酶活及其固定化细胞对农药的降解

将抗性尖音库蚊五带亚种的执有机磷农药基因—酯酶基因克隆到质粒pRL－439中．经检测,重组质粒中的酯酶基因已得到表达,获得高酶活的工程菌．将工程菌固定化后对两类难降解农药（有机氯、菊酯类）进行降解,结果表明,固定化细胞在1h内对上两类农药可降解90％以上,具有很高的降解效力．     

The transatlantic rift in genetically modified food policy

The regulatory structures underlying United States and European Union policies regarding genetically modified (GM) food and crops are fundamentally different. The US regulates GM foods and crops as end products, applying roughly the same regulatory framework that it does to non GM foods or crops. The EU, on the other hand, regulates products of agricultural biotechnology as the result of a specific production process. Accordingly, it has developed a network of rules that regulate GM foods and crops specifically. As a result, US regulation of GM foods and crops is relatively permissive, whereas EU regulation is relatively restrictive. Why are genetically modified food policies in the United States and the European Union so strikingly different? In the light of the recent World Trade Organization dispute on agricultural biotechnology, it may seem that economic interests are the driving force behind policies. While they are certainly part of the picture, the issue is far more complex. This paper argues that three different elements help explain differences between US and EU GM food policies. First, an investigation of US and European policies of the 1970s and 1980s on recombinant DNA research and of events leading up to early GM food and crop regulation allows a deeper understanding of current policy. Second, scrutinizing underlying values and norms can uncover the beliefs that condition current GM food and crop policy. Third, an analysis of involved actors’ views and levels of success in influencing policy is essential to understanding US and EU policies.     

论活性污泥微生物指示作用

为研究石化污水处理场生化处理过程中微生物相的特性与曝气池运行状态、处理效果之间的关系,选用锦西石化分公司污水处理场合建式完全混合曝气池为研究对象,以其活性污泥做样品直接进行镜检。通过观察丝状菌、游离细菌、钟虫、变形虫、鞭毛虫生长繁殖变化,分析各种微生物对应水质条件,得出活性污泥微生物对合建式曝气池运行状态和水质的指示规律。     

土壤和食物中砷生物可给性与生物有效性研究进展

砷是一种环境中广泛存在致癌元素。土壤中的砷污染不仅会影响作物的产量和质量,而且还可通过食物链扩散到人体,严重威胁人类健康。近些年来,研究人员对砷生物有效性和可给性开展了系列相关研究。本文对重金属砷的来源与危害进行了归纳;对砷生物可给性与生物有效性的概念及两者之间的联系进行了总结;对in vivo、Caco-2细胞模型以及7种in vitro方法进行了分析概括;总结分析了不同因素（砷浓度及其形态、矿物质元素、营养状态和结肠微生物等）对土壤和食物中砷生物可给性和生物有效性产生的影响。最后,本文对未来砷生物有效性与可给性的评估及砷污染机制探索工作进行了展望,提出标准化体外胃肠模型的应用将使砷健康风险评估结果更为可靠。     

外源洛克沙胂对水生态系统砷蓄积及底泥微生物生长的影响

为探明洛克沙胂(ROX)对水生态系统的毒性效应,采用模拟水生态系统,研究了外源添加不同浓度洛克沙胂(0、10、20、40、80和160 mg·L-1)对水生态系统砷含量及底泥微生物生长的影响。结果表明,水体及底泥砷含量随外源ROX用量增加而增加,但随暴露时间延长水体砷含量降低的同时底泥砷含量逐渐增加。金鱼藻和鲫鱼体内均出现明显砷蓄积现象,且160 mg·L-1ROX处理的水生态系统鲫鱼毒害效应明显,暴露1 d的死亡率为100%。金鱼藻对砷具有较强的富集能力,暴露32 d后砷富集量达398.1~1 538.91 mg·kg-1。不同浓度ROX对底泥真菌、细菌和反硝化细菌生长均具有不同程度的抑制效应,而对放线菌和氨化细菌生长具有一定的促进作用,且低浓度ROX(10 mg·L-1)对放线菌生长的促进作用明显。总体上,外源ROX进入水生态系统导致水体砷污染的同时在生物及非生物媒介中再次迁移、分配和蓄积,进而对鲫鱼及部分底泥微生物生长产生毒害。     

纳米银颗粒在水环境中的环境行为和毒性效应研究进展

纳米材料的环境行为和生态效应是目前国内外研究的热点,其中纳米银颗粒(Ag NPs)是使用量最高的纳米材料。本文主要总结了Ag NPs在水环境中的赋存、Ag NPs的环境行为、Ag NPs对不同种类微生物的毒性效应以及影响Ag NPs毒性效应的因素,最后对Ag NPs在河口区的研究进行了展望。     

利用有效微生物菌群控制蓝藻水华研究

蓝藻水华污染在我国和世界都呈现迅猛的发展趋势,如何治理是环境科学研究领域的热点。2008年7～8月在北京市延庆县妫水湖的现场围隔水体中,通过投加筛选和培养土著有效微生物菌群(EM菌),对控制蓝藻水华污染形成的效果进行研究。结果表明,与对照相比,投加EM菌不仅可以控制蓝藻水华的暴发,使蓝藻生物量降低70%以上,而且可以快速降低水体中的氮和磷浓度,因此,明显控制了蓝藻水华的形成,在治理湖泊蓝藻水华污染方面具有重要的应用前景。     

The Political Import of Intrinsic Objections to Genetically Engineered Food

Many people object to genetically engineerehd (GE) food because they believe that it is unnatural or that its creation amounts to playing God. These objections are often referred to as intrinsic objections, and they have been widely criticized in the agricultural bioethics literature as being unsound, incompatible with modern science, religious, inchoate, and based on emotion instead of reason. Many of their critics also argue that even if these objections did have some merit as ethicalobjections, their quasi-religious nature means that they are entirely irrelevant when interpreted aspolitical objections regarding what public policy ought to be. In this paper, we argue that this widespread view is false. Intrinsic objections have much more political import than has previously been recognized, and indeed the requirements of political liberalism and its associated idea of liberal neutrality, once properly understood, protect intrinsic objections from many of the most common objections. That is, policy-makers may not legitimately base public policy on grounds that are inconsistent with intrinsic objections, even when they believe those objections to be flawed in the ways mentioned above. This means that in the context of a political debate about GE food, the discussion should not center on the substantive merits of the intrinsic objections themselves but rather on the appropriate political norms for achieving democratically legitimate policy on issues that touch people’s deepest religious and moral beliefs.