Matrine and oxymatrine in corroborant plant extracts and fertilizers: HPLC/MS-MS method development and single-laboratory validation

A reversed phase high-performance liquid chromatographic method (HPLC/MS-MS) has been developed and validated for detection of alkaloids matrine and oxymatrine in fertilizer with labeled enhancer plant defense activities. The analytical method was validated statistically. The results show a strong matrix effect, requiring quantification by standard addition method. The regression lines showed r² > 0.994. Recoveries ranging from 97 to 104% were obtained for the fortification level of 0.01% wt wt^?1 and the relative standard deviations ranged from 3 to 4% (n = 10). The limits of detection were below 0.0001% wt wt^?1, while the limits of quantification did not exceed 0.0004% wt wt^?1. The method is currently applied in ICQRF Laboratory of Catania on fertilized and corroborant plant extract collected in the Italian market in the frame of MIPAAF institutional quality control activity, with the aim to dectect these unpermitted active substances.     

Structural changes in soil communities after triclopyr application in soils invaded by Acacia dealbata Link

Triclopyr is a commonly used herbicide in the control of woody plants and can exhibit toxic effects to soil microorganisms. However, the impact on soils invaded by plant exotics has not yet been addressed. Here, we present the results of an 18-month field study conducted to evaluate the impact of triclopyr on the structure of fungal and bacterial communities in soils invaded by Acacia dealbata Link, through the use of denature gradient gel electrophoresis. After triclopyr application, analyses of bacterial fingerprints suggested a change in the structure of the soil bacterial community, whereas the structure of the soil fungal community remained unaltered. Bacterial density and F:B ratio values changed across the year but were not altered due to herbicide spraying. On the contrary, fungal diversity was increased in plots sprayed with triclopyr 5 months after the first application. Richness and diversity (H´) of both bacteria and fungi were not modified after triclopyr application.     

对普光气田油菜的二氧化硫熏气实验

为探讨普光气田废气对周围农作物的影响和生物监测方法,选择气田周边油菜作为受试样本,对其进行二氧化硫现场熏气实验。结果表明,油菜在受到1.43 mg/m3浓度、3小时二氧化硫熏气后,植株耐受,不产生明显受害症状;叶片含硫量、MDA含量、可溶性蛋白含量等指标的测定结果与二氧化硫熏气浓度、熏气时间存在显著的相关关系。     

快速杀灭紫茎泽兰的新技术研究

主要针对外来入侵植物紫茎泽兰的快速杀灭新技术进行研究。利用植物对某些营养元素的敏感性特点,研制出紫茎泽兰专用生长抑制剂。采用喷洒紫茎泽兰生长抑制剂的方式,达到抑制紫茎泽兰生长直至杀灭的目的,以及采用在原地种植替代植物的方式防止土地再次被紫茎泽兰侵占。     

低碳城市理念下的郑州市绿地系统优化研究

低碳城市是一种实现低碳经济、进行低碳生产与消费、形成低碳生活、建设良性与可持续发展的能源生态体系的城市模式。通过调控城市绿地系统、优化城市开放空间,有利于低碳城市理念下的生态城市建设,实现城市的可持续发展。首先对低碳城市建设与城市绿地系统优化的关系进行了分析;其次对郑州城市绿地系统建设存在的主要问题进行了探讨,借助AreGIS9．3软件分析了郑州市绿地服务区的影响范围;最后基于城市绿地系统优化原则提出四方面的优化建议——注重城市的生态设计;优化绿地系统的空间布局;实现“点”、“线”、“面”的有机结合;实施适度的绿地容量限制;发展立体绿化与推广绿色建筑材料的使用。     

污水处理厂AB法工艺改造方案的探讨

针对漳州市东区污水处理厂AB法工艺改造的目标,分析了AB法工艺的局限性,结合该污水处理厂AB法工程的现有设施,从技术上探讨了5种具有脱氮除磷功能的工艺改造方案的可行性,并得到了适合于该污水处理厂AB法工艺改造的方案。     

绿色油田建设中存在的问题及对策探讨

文章分析了石油开发对环境的影响,提出了绿色油田建设的必要性与重要性。通过对国内外绿色油田建设现状的调研与对比,总结出目前我国建设绿色油田的瓶颈,提出了建议与对策,并展望了建设绿色油田的前景。     

Eichhornia crassipes capability to remove naphthalene from wastewater in the absence of bacteria

The aim of the current study was to investigate the potential of an aquatic plant, the water hyacinth (Eichhornia crassipes) devoid rhizospheric bacteria, to reduce naphthalene (a polyaromatic hydrocarbon) present in wastewater and wetlands.The capability of sterile water hyacinth plants to remove naphthalene from water and wastewater was studied in batch systems. Water hyacinths enhance the removal of pollutants through their consumption as nutrients and also through microbial activity of their rhizospheric bacteria.Experimental kinetics of naphthalene removal by water hyacinth coupled with natural rhizospheric bacteria was 100% after 9 d. Plants, decoupled of rhizospheric bacteria, reduced naphthalene concentration up to 45% during 7 d. Additionally, naphthalene uptake by water hyacinth revealed a biphasic behavior: a rapid first phase completed after 2.5 h, and a second, considerably slower rate, phase (2.5-225 h). In conclusion, water hyacinth devoid rhizospheric bacteria reduced significantly naphthalene concentration in water, revealing a considerable plant contribution in the biodegradation process of this pollutant.     

Nitrogen fertilizer improves boron phytoextraction by Brassica juncea grown in contaminated sediments and alleviates plant stress

In this study we evaluated the effect of different fertilizer treatments on Brassica plants grown on boron-contaminated sediments. Experiments were conducted in the laboratory and on the lysimeter scale. At laboratory scale (microcosm), five different fertilizers were tested for a 35-d period. On the lysimeter scale, nitrogen fertilization was tested at three different doses and plants were allowed to grow until the end of the vegetative phase (70 d). Results showed that nitrogen application had effectively increased plant biomass production, while B uptake was not affected. Total B phytoextracted increased three-fold when the highest nitrogen dose was applied. Phytotoxicity on Brassica was evaluated by biochemical parameters. In plants grown in unfertilized B-contaminated sediments, the activity of antioxidant enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and pyrogallol peroxidase (PPX) increased, whereas catalase (CAT) decreased with respect to control plants. Addition of N progressively mitigated the alteration of enzymatic activity, thus suggesting that N can aid in alleviating B-induced oxidative stress. SOD activity was restored to control levels just at the lowest N treatment, whereas the CAT inhibition was partially restored only at the highest one. N application also lowered the B-induced increase in APX and PPX activities. Increased glutathione reductase activity indicated the need to restore the oxidative balance of glutathione. Data also suggest a role of glutathione and phytochelatins in B defense mechanisms. Results suggest that the nitrogen fertilizer was effective in improving B phytoextraction by increasing Brassica biomass and by alleviating B-induced oxidative stress.     

Estimation of thyroid doses and health risks resulting from the intake of radioactive iodine in foods and drinking water by the citizens of Tokyo after the Fukushima nuclear accident

The release of radioactive materials from the Fukushima nuclear power plant after the Great East Japan Earthquake on 11 March 2011 poses health risks. In this study, the intake of iodine 131 (I-131) in drinking water and foods (milk, dairy products, and vegetables) by citizens of Tokyo was estimated. The effects of countermeasures (restrictions on the distribution of foods and the distribution of bottled water for infants) on reducing intake were also evaluated. The average thyroid equivalent doses without countermeasures from 21 March 2011 were 0.42 mSv in adults, 1.49 mSv in children, and 2.08 mSv in infants. Those with countermeasures were 0.28, 0.97, and 1.14 mSv respectively, reductions of 33%, 35%, and 45%. Drinking water contributed more to intake by adults and children than foods. The intake of I-131 within the first 2 weeks was more than 80% of the estimated intake, owing to its short half-life, indicating that rapid countermeasures are important in reducing intake. The average risks of cancer incidence and mortality due to I-131 for infants were estimated to be 3 × 10⁻⁵ and 0.2 × 10⁻⁵, respectively, lower than the annual risks of traffic accidents, naturally occurring radioactive material (potassium 40), and environmental pollutants such as diesel exhaust particles.